Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,738,525	C→A	R228S (CGC→AGC)	purR →	DNA‑binding transcriptional repressor PurR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,738,525	0	C	A	100.0%	290.4 / NA	82	R228S (CGC→AGC)	purR	DNA‑binding transcriptional repressor PurR
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (32/50);  total (32/50)

GCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGG  >  NC_000913/1738388‑1738661                                                                                                                                          |                                                                                                                                         gCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATagc                                                                                                                                        >  1:1215176/1‑140 (MQ=255)        ccccGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATgc                                                                                                                                 <  2:933678/140‑1 (MQ=255)            ggCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATgcagca                                                                                                                             <  1:819679/140‑1 (MQ=255)               ccGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAAt                                                                                                                          <  1:94744/140‑1 (MQ=255)                 gCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATcc                                                                                                                        >  1:1059977/1‑140 (MQ=255)                 gCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATcc                                                                                                                        >  1:772355/1‑140 (MQ=255)                 gCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATcc                                                                                                                        >  2:1415834/1‑140 (MQ=255)                  cTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCt                                                                                                                       >  1:484374/1‑140 (MQ=255)                  cTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCt                                                                                                                       >  2:662428/1‑140 (MQ=255)                    ggAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGt                                                                                                                     <  2:484374/140‑1 (MQ=255)                         gTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAg                                                                                                                <  2:1086364/140‑1 (MQ=255)                            acacCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCg                                                                                                             <  2:931556/140‑1 (MQ=255)                            acacCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCg                                                                                                             >  1:1410977/1‑140 (MQ=255)                            acacCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCg                                                                                                             <  2:1237100/140‑1 (MQ=255)                              acCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCa                                                                                                           <  1:1165928/140‑1 (MQ=255)                              acCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCa                                                                                                           <  1:153500/140‑1 (MQ=255)                               ccGGCGCAGGCCGCCTTGCCTGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                                                                                                          <  2:1410977/140‑1 (MQ=255)                               ccGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                                                                                                          <  1:1415834/140‑1 (MQ=255)                               ccGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                                                                                                          <  2:386049/140‑1 (MQ=255)                               ccGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                                                                                                          >  2:174383/1‑140 (MQ=255)                               ccGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCAt                                                                                                          <  1:9819/140‑1 (MQ=255)                                   cgcAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGcc                                                                                                      >  1:855382/1‑140 (MQ=255)                                     cAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCt                                                                                                    >  1:182949/1‑140 (MQ=255)                                       ggCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTAc                                                                                                  >  2:1338427/1‑140 (MQ=255)                                        gccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACt                                                                                                 >  1:731733/1‑140 (MQ=255)                                        gccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACt                                                                                                 >  1:621218/1‑140 (MQ=255)                                         ccgccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTg                                                                                                <  1:557752/140‑1 (MQ=255)                                           gccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGcc                                                                                              <  2:316511/140‑1 (MQ=255)                                            ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGcc                                                                                              <  2:969810/139‑1 (MQ=255)                                            ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGcc                                                                                              >  1:969810/1‑139 (MQ=255)                                            ccTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCA‑GGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGt                                                                                            >  1:496150/1‑140 (MQ=255)                                             cTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGcc                                                                                              >  2:1260876/1‑138 (MQ=255)                                             cTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGcc                                                                                              <  1:1260876/138‑1 (MQ=255)                                                gCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtctt                                                                                         <  2:1329328/140‑1 (MQ=255)                                                  cGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtct                                                                                          <  2:690959/137‑1 (MQ=255)                                                  cGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGtct                                                                                          >  1:690959/1‑137 (MQ=255)                                                     ttttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCtgtg                                                                                    <  1:1338427/140‑1 (MQ=255)                                                      tttATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtgg                                                                                   <  2:855382/140‑1 (MQ=255)                                                        tATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTgtggtg                                                                                 >  2:67287/1‑140 (MQ=255)                                                         aTGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGtggtgg                                                                                <  2:1181000/140‑1 (MQ=255)                                                          tGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGc                                                                               <  1:783935/140‑1 (MQ=255)                                                          tGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGc                                                                               <  1:174383/140‑1 (MQ=255)                                                          tGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGc                                                                               <  2:1446597/140‑1 (MQ=255)                                                           gAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCg                                                                              <  2:666882/140‑1 (MQ=255)                                                            aaGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGa                                                                             <  2:621218/140‑1 (MQ=255)                                                            aaGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGa                                                                             <  1:1060660/140‑1 (MQ=255)                                                              ggCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGata                                                                           <  1:1428336/140‑1 (MQ=255)                                                               gCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGatat                                                                          >  1:232487/1‑140 (MQ=255)                                                                cGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATc                                                                         <  1:1054667/140‑1 (MQ=255)                                                                 gATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCa                                                                        >  2:483232/1‑140 (MQ=255)                                                                           gCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATggg                                                              <  1:1204915/140‑1 (MQ=255)                                                                            cGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGgc                                                             <  2:1335175/140‑1 (MQ=255)                                                                             gatgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGgc                                                             <  1:1318105/139‑1 (MQ=255)                                                                             gatgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGgc                                                             >  2:1318105/1‑139 (MQ=255)                                                                              atgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGgcgc                                                           >  2:1329757/1‑140 (MQ=255)                                                                              atgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGgcgc                                                           >  1:356178/1‑140 (MQ=255)                                                                               tgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCa                                                          >  1:1389297/1‑140 (MQ=255)                                                                               tgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCa                                                          <  2:966325/140‑1 (MQ=255)                                                                               tgatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCa                                                          <  2:853363/140‑1 (MQ=255)                                                                                gatCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCAc                                                         >  2:1025734/1‑140 (MQ=255)                                                                                    aaGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtg                                                     <  2:1096214/140‑1 (MQ=255)                                                                                    aaGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtg                                                     <  2:28180/140‑1 (MQ=255)                                                                                      ggTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTtgtg                                                   <  2:1133738/140‑1 (MQ=255)                                                                                           cGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct                                              >  1:1178562/1‑140 (MQ=255)                                                                                           cGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTgctgct                                              >  2:85832/1‑140 (MQ=255)                                                                                                  cTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGaaa                                       >  2:703171/1‑140 (MQ=255)                                                                                                            cAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTgcg                             <  1:67287/140‑1 (MQ=255)                                                                                                            cAGGGTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTgcg                             >  1:1174947/1‑140 (MQ=255)                                                                                                                gTGACTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTc                         <  1:242004/140‑1 (MQ=255)                                                                                                                    cTTTGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGc                     >  2:1464645/1‑140 (MQ=255)                                                                                                                     tttGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCa                    <  2:356178/140‑1 (MQ=255)                                                                                                                     tttGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCa                    <  2:1389297/140‑1 (MQ=255)                                                                                                                       tGAACCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAgg                  <  1:85832/140‑1 (MQ=255)                                                                                                                         aaCCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCTCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCa                    <  2:408528/136‑1 (MQ=255)                                                                                                                         aaCCTGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCTCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCa                    >  1:408528/1‑136 (MQ=255)                                                                                                                             tGAATCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGttt            <  1:1004445/140‑1 (MQ=255)                                                                                                                               aaTCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTG‑‑‑TGCTGATGAAAT‑GGCCTGCGCGTCCCGCAGGATGTTTCGCTGa      <  2:232487/140‑1 (MQ=255)                                                                                                                                 tCCGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCt        >  2:881934/1‑140 (MQ=255)                                                                                                                                   cGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGa      <  1:1464645/140‑1 (MQ=255)                                                                                                                                   cGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGa      <  2:779424/140‑1 (MQ=255)                                                                                                                                   cGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGa      <  2:19648/140‑1 (MQ=255)                                                                                                                                   cGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGa      <  2:182949/140‑1 (MQ=255)                                                                                                                                   cGGTTATAGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGa      >  1:1098736/1‑140 (MQ=255)                                                                                                                                       tataGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCgg  <  1:703171/140‑1 (MQ=255)                                                                                                                                       tataGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCgg  <  1:881934/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         GCGTCATCCCCGGCCCGCTGGAACGTAACACCGGCGCAGGCCGCCTTGCCGGTTTTATGAAGGCGATGGAAGAAGCGATGATCAAGGTGCCGGAAAGCTGGATTGTGCAGGGTGACTTTGAACCTGAATCCGGTTATCGCGCCATGCAGCAAATCCTGTCGCAGCCGCATCGCCCTACTGCCGTCTTCTGTGGTGGCGATATCATGGCAATGGGCGCACTTTGTGCTGCTGATGAAATGGGCCTGCGCGTCCCGCAGGATGTTTCGCTGATCGG  >  NC_000913/1738388‑1738661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑