Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,599,317	C→A	V148V (GTG→GTT)	dapA ←	4‑hydroxy‑tetrahydrodipicolinate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,599,317	0	C	A	100.0%	265.6 / NA	76	V148V (GTG→GTT)	dapA	4‑hydroxy‑tetrahydrodipicolinate synthase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/40);  total (36/40)

TCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGACGATTGTAGTAAGGGGT  >  NC_000913/2599183‑2599451                                                                                                                                       |                                                                                                                                       tCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGt                                                                                                                                   >  1:500931/1‑140 (MQ=255)   cAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGttt                                                                                                                                 >  1:1417726/1‑140 (MQ=255)    aGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTc                                                                                                                                >  1:798574/1‑140 (MQ=255)        cgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCg                                                                                                                                    >  1:696190/1‑132 (MQ=255)        cgcTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCg                                                                                                                                    <  2:696190/132‑1 (MQ=255)          cTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAg                                                                                                                          >  1:296999/1‑140 (MQ=255)                tcatcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATc                                                                                                                    <  2:1118528/140‑1 (MQ=255)                tcatcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATc                                                                                                                    <  2:445588/140‑1 (MQ=255)                   tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAGGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCa                                                                                                                 <  2:1294258/140‑1 (MQ=255)                   tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCa                                                                                                                 <  1:215196/140‑1 (MQ=255)                   tcGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCa                                                                                                                 <  1:385447/140‑1 (MQ=255)                     gccgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGc                                                                                                               <  2:752647/140‑1 (MQ=255)                       cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCa                                                                                                             >  2:1365378/1‑140 (MQ=255)                       cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCa                                                                                                             >  2:1235974/1‑140 (MQ=255)                       cgcTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCa                                                                                                             >  1:850767/1‑140 (MQ=255)                         cTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGt                                                                                                           >  1:934313/1‑140 (MQ=255)                          tCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTa                                                                                                          <  1:209968/140‑1 (MQ=255)                            agcagAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACg                                                                                                        <  2:1417726/140‑1 (MQ=255)                               agAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGa                                                                                                     <  2:13625/140‑1 (MQ=255)                                 aaCAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACg                                                                                                   <  1:1245959/140‑1 (MQ=255)                                   cAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGc                                                                                                 <  2:296999/140‑1 (MQ=255)                                    aaaaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGca                                                                                                <  2:1270545/140‑1 (MQ=255)                                      aaTCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGcaca                                                                                              <  1:528093/140‑1 (MQ=255)                                           tcTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATtata                                                                                         >  2:1390895/1‑140 (MQ=255)                                                    aGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTg                                                                                >  2:1451119/1‑140 (MQ=255)                                                      ctctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCg                                                                              >  1:66910/1‑140 (MQ=255)                                                       tctTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTCCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCgg                                                                             <  2:934313/140‑1 (MQ=255)                                                         tttGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa                                                                           <  2:850767/140‑1 (MQ=255)                                                                tGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACGGAATTTGCGGCAGGTCAGt                                                                    >  2:778413/1‑140 (MQ=255)                                                                tGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGt                                                                    >  2:544110/1‑140 (MQ=255)                                                                tGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGt                                                                    <  1:1390895/140‑1 (MQ=255)                                                                   tttACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa                                                                           >  1:483101/1‑130 (MQ=255)                                                                   tttACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCa                                                                           <  2:483101/130‑1 (MQ=255)                                                                    ttACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGc                                                                >  2:244230/1‑140 (MQ=255)                                                                     tACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCt                                                               >  1:596555/1‑140 (MQ=255)                                                                       cacGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCa                                                             >  1:1422680/1‑140 (MQ=255)                                                                       cacGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCa                                                             >  1:349209/1‑140 (MQ=255)                                                                       cacGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCa                                                             >  1:1005490/1‑140 (MQ=255)                                                                           cgTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa                                                         <  2:73038/140‑1 (MQ=255)                                                                           cgTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa                                                         <  2:349209/140‑1 (MQ=255)                                                                           cgTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGa                                                         <  1:1259721/140‑1 (MQ=255)                                                                            gTTAAGTTCCCTGTTGCCTCTTTGATTCTGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGc                                                                <  1:1007070/132‑1 (MQ=255)                                                                            gTTAAGTTCCCTGTTGCCTCTTTGATTCTGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGc                                                                >  2:1007070/1‑132 (MQ=255)                                                                            gTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGAt                                                        <  2:596555/140‑1 (MQ=255)                                                                            gTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGAt                                                        >  2:221737/1‑140 (MQ=255)                                                                            gTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGAt                                                        >  2:822609/1‑140 (MQ=255)                                                                             ttAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATg                                                       >  1:549461/1‑140 (MQ=255)                                                                                aGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCt                                                    <  1:822609/140‑1 (MQ=255)                                                                                aGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCACAGCCAGTACGAGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCt                                                    >  2:403411/1‑140 (MQ=255)                                                                                    cccTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGa                                                <  2:191691/140‑1 (MQ=255)                                                                                      cTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCAATGGCTTTGaaa                                              >  1:176886/1‑140 (MQ=255)                                                                                        gTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATg                                            <  2:1005490/140‑1 (MQ=255)                                                                                            ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATgg                                                      <  2:789227/126‑1 (MQ=255)                                                                                            ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATgg                                                      >  1:789227/1‑126 (MQ=255)                                                                                            ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGa                                        >  2:592768/1‑140 (MQ=255)                                                                                            ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGa                                        >  2:466468/1‑140 (MQ=255)                                                                                            ccTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGa                                        >  1:1380792/1‑140 (MQ=255)                                                                                                 ttGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACaa                                   >  1:336684/1‑140 (MQ=255)                                                                                                   gATTCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAAc                                 >  2:526379/1‑140 (MQ=255)                                                                                                      tCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACctt                              <  1:244230/140‑1 (MQ=255)                                                                                                      tCCGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACctt                              >  1:409925/1‑140 (MQ=255)                                                                                                       ccGATAATATTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACcttc                             <  1:1451119/140‑1 (MQ=255)                                                                                                          ataataTTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTg                          >  2:969730/1‑140 (MQ=255)                                                                                                            aataTTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCg                        <  2:66910/140‑1 (MQ=255)                                                                                                              tatTTTTTACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgac                      >  2:76411/1‑140 (MQ=255)                                                                                                                ttttttACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgg                    <  2:500931/140‑1 (MQ=255)                                                                                                                ttttttACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgg                    <  1:466468/140‑1 (MQ=255)                                                                                                                 tttttACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACGGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga                   <  1:778413/140‑1 (MQ=255)                                                                                                                 tttttACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga                   <  2:409925/140‑1 (MQ=255)                                                                                                                 tttttACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga                   <  1:592768/140‑1 (MQ=255)                                                                                                                 tttttACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga                   <  2:1422680/140‑1 (MQ=255)                                                                                                                  ttttACTTTCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCAATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacggac                  <  2:176886/140‑1 (MQ=255)                                                                                                                        tttCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga                   >  2:518265/1‑133 (MQ=255)                                                                                                                        tttCGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCgacgga                   <  1:518265/133‑1 (MQ=255)                                                                                                                           cGCCAGACGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGACGATTgtagt         <  1:221737/140‑1 (MQ=255)                                                                                                                                  cGGCCAACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGACGATTGTAGTAAGGGGt  <  2:1380792/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TCCAGCGCGCTCGCATCATCGCCGCTCAGCAGAACAAAATCATCTGAAACCAGCTCTTTGATCTGGTTTACACGCGTTAAGTTCCCTGTTGCCTCTTTGATTCCGATAATATTTTTTACTTTCGCCAGACGGCCCACCGTTTCCGGGAGCAGATCGCAGCCAGTACGGGACGGCACATTATACAGAATTTGCGGCAGGTCAGTATGCTCAGCGATGGCTTTGAAATGCTGATACAAACCTTCTTGCGACGGACGATTGTAGTAAGGGGT  >  NC_000913/2599183‑2599451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑