Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,919,999	G→T	R135R (CGC→CGA)	atpH ←	ATP synthase F1 complex subunit delta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,919,999	0	G	T	100.0%	240.5 / NA	68	R135R (CGC→CGA)	atpH	ATP synthase F1 complex subunit delta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (40/28);  total (40/28)

TCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAA  >  NC_000913/3919865‑3920129                                                                                                                                       |                                                                                                                                   tCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGa                                                                                                                               >  2:620386/1‑140 (MQ=255)   cccTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACa                                                                                                                             <  1:620715/140‑1 (MQ=255)     cTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGa                                                                                                                           >  2:227325/1‑140 (MQ=255)      ttAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCg                                                                                                                                  >  2:1006228/1‑132 (MQ=255)      ttAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACa                                                                                                                             <  2:1465190/137‑1 (MQ=255)      ttAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACa                                                                                                                             >  1:1465190/1‑137 (MQ=255)      ttAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGAc                                                                                                                          >  2:239436/1‑140 (MQ=255)             tGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGAc                                                                                                                              <  2:1312852/129‑1 (MQ=255)             tGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGAc                                                                                                                              >  1:1312852/1‑129 (MQ=255)             tGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTc                                                                                                                   >  2:540866/1‑140 (MQ=255)             tGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTc                                                                                                                   >  2:1278093/1‑140 (MQ=255)             tGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTc                                                                                                                   >  1:900474/1‑140 (MQ=255)                  gACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCg                                                                                                              <  1:257920/140‑1 (MQ=255)                        tGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGcagcag                                                                                                        <  1:620386/140‑1 (MQ=255)                         gCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGa                                                                                                       >  1:236728/1‑140 (MQ=255)                           aaGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGaaa                                                                                                     <  1:1312255/140‑1 (MQ=255)                             ggCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAAtt                                                                                                   >  1:270302/1‑140 (MQ=255)                               cgcTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAAtttt                                                                                                 >  1:263776/1‑140 (MQ=255)                                 cTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTcg                                                                                               >  2:1276177/1‑140 (MQ=255)                                   cAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTcgcg                                                                                             <  1:436208/140‑1 (MQ=255)                                   cAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTcgcg                                                                                             <  1:386753/140‑1 (MQ=255)                                       acgacCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCt                                                                                         <  1:798498/140‑1 (MQ=255)                                       acgacCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCt                                                                                         <  2:772065/140‑1 (MQ=255)                                         gacCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCtgt                                                                                       >  2:893344/1‑140 (MQ=255)                                           ccGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCtgttg                                                                                     >  1:36049/1‑140 (MQ=255)                                           ccGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATAATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCtgttg                                                                                     >  2:587405/1‑140 (MQ=255)                                               gTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCa                                                                                 <  1:893344/140‑1 (MQ=255)                                                       ccATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGc                                                                         >  2:1387594/1‑140 (MQ=255)                                                       ccATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGc                                                                         <  2:900474/140‑1 (MQ=255)                                                         aTCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCgg                                                                       >  2:1414227/1‑140 (MQ=255)                                                                 ccATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGaa                                                               >  1:1051521/1‑140 (MQ=255)                                                                 ccATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGaa                                                               >  1:1093997/1‑140 (MQ=255)                                                                      tCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGAc                                                          <  2:1362895/140‑1 (MQ=255)                                                                        aCCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGt                                                        <  2:36049/140‑1 (MQ=255)                                                                        aCCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGt                                                        >  1:373969/1‑140 (MQ=255)                                                                          ccGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCt                                                      >  1:771183/1‑140 (MQ=255)                                                                           cGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTa                                                     >  1:1351358/1‑140 (MQ=255)                                                                            gCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTAc                                                    >  1:288187/1‑140 (MQ=255)                                                                                    gatAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCgg                                            <  2:771183/140‑1 (MQ=255)                                                                                    gatAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCgg                                            <  2:373969/140‑1 (MQ=255)                                                                                    gatAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCgg                                            <  1:1414227/140‑1 (MQ=255)                                                                                     atAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGt                                           <  2:263776/140‑1 (MQ=255)                                                                                            cTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctca                                    >  1:1376054/1‑140 (MQ=255)                                                                                            cTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctca                                    >  2:1285856/1‑140 (MQ=255)                                                                                            cTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctca                                    >  1:1160951/1‑140 (MQ=255)                                                                                             tGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcac                                   <  1:1285856/140‑1 (MQ=255)                                                                                              gCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcact                                  >  1:889511/1‑140 (MQ=255)                                                                                              gCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcact                                  <  1:540866/140‑1 (MQ=255)                                                                                               ccATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcactc                                 >  1:829030/1‑140 (MQ=255)                                                                                               ccATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATAGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcactc                                 >  1:713050/1‑140 (MQ=255)                                                                                                cATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCctcactca                                >  1:1108576/1‑140 (MQ=255)                                                                                                  ttACAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACg                              >  2:229724/1‑140 (MQ=255)                                                                                                    aCAGACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGc                            >  2:1186933/1‑140 (MQ=255)                                                                                                       gACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                         <  1:1278093/140‑1 (MQ=255)                                                                                                       gACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                         <  2:1376054/140‑1 (MQ=255)                                                                                                       gACTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                         <  2:889511/140‑1 (MQ=255)                                                                                                         cTTATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCCACCTCAGCGGTAGCCTCACTCACGGCACGCa                       <  1:587405/140‑1 (MQ=255)                                                                                                          ttATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                         <  2:1330113/137‑1 (MQ=255)                                                                                                          ttATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACg                         >  1:1330113/1‑137 (MQ=255)                                                                                                           tATCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAgg                     >  1:165741/1‑140 (MQ=255)                                                                                                            aTCGATTTTGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGt                    >  1:486534/1‑140 (MQ=255)                                                                                                                  tttGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATaa              >  2:78233/1‑140 (MQ=255)                                                                                                                    tGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAAc            <  2:486534/140‑1 (MQ=255)                                                                                                                    tGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAAc            >  1:1247679/1‑140 (MQ=255)                                                                                                                    tGCAATTCAGCTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAAc            <  1:1385061/140‑1 (MQ=255)                                                                                                                              cTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCa     <  2:944891/137‑1 (MQ=255)                                                                                                                              cTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCa     >  1:944891/1‑137 (MQ=255)                                                                                                                              cTTAACTTTTCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGaa  >  1:1064833/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   TCCCCTTAAGACTGCAAGACGTCTGCAAGGCGCTCAAGACGACCGCGTACGCTGCCATCAATGACCATATCACCCGCTCGGATGATAACGCCTGCCATTACAGACTTATCGATTTTGCAATTCAGCTTAACTTTGCGTGACAGACGTTTTTCCATCGCAGCAGAAATTTTCGCGAGCTGTTGTTCACTCAGTGCGGCAGCGGAAATGACGTCTACCTCAGCGGTAGCCTCACTCACGGCACGCAGGTGAATAAACTGCTCCAGAA  >  NC_000913/3919865‑3920129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑