Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	364,052	G→T	L752M (CTG→ATG)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	364,052	0	G	T	100.0%	67.4 / NA	21	L752M (CTG→ATG)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (6/15);  total (6/15)

TACGCCAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCAT  >  NC_000913/363918‑364182                                                                                                                                       |                                                                                                                                   tACGCCAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGa                                                                                                                               <  2:323969/140‑1 (MQ=255) tACGCCAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGa                                                                                                                               <  2:1450081/140‑1 (MQ=255)      cAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCaa                                                                                                                          <  1:1223895/140‑1 (MQ=255)                      cGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAAACCCTTTTGCtgg                                                                                                          >  1:1163318/1‑140 (MQ=255)                          gCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTggtggt                                                                                                      <  1:1311631/140‑1 (MQ=255)                           cACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCCACGCTTTTTACCCCTCTCGATGCAAAAAACCATTTCGCtggggggc                                                                                                     >  1:109740/1‑138 (MQ=255)                           cACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTc                                                                                                     <  1:554028/140‑1 (MQ=255)                             cGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAg                                                                                                   <  1:320156/140‑1 (MQ=255)                                    aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                                                                                            <  2:680197/140‑1 (MQ=255)                                    aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                                                                                            <  1:171909/140‑1 (MQ=255)                                    aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                                                                                            <  2:1466688/140‑1 (MQ=255)                                    aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                                                                                            <  2:1410351/140‑1 (MQ=255)                                    aCTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggg                                                                                            <  2:1323282/140‑1 (MQ=255)                                        aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggaa                                                                                           <  2:764622/137‑3 (MQ=255)                                        aTCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCggaa                                                                                           >  1:764622/1‑135 (MQ=255)                                                      tCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACgcggcg                                                                          <  2:167605/140‑1 (MQ=255)                                                       cagcagTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTTCCAACGCTTATTACCCCTCTCTATGCAAAAAACCATTTCGCTTGTGGTCAGATGCGGGGTGGCGTGGGGCGcggcgg                                                                         >  2:1007417/1‑140 (MQ=255)                                                             ttgttTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCg                                                                   <  1:8215/140‑1 (MQ=255)                                                                                     cTGTGAAAGAAAGCCTGACTGGGGGTTAAATTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGAc                                           >  1:1018768/1‑140 (MQ=255)                                                                                                                aaaTTGCCAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGcc                <  1:1413583/140‑1 (MQ=255)                                                                                                                      ccAACGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCtt          >  2:761593/1‑140 (MQ=255)                                                                                                                        aaCGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCt        >  1:345187/1‑140 (MQ=255)                                                                                                                         aCGCTTATTACCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTg       >  2:1444958/1‑140 (MQ=255)                                                                                                                              tattaCCCATCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCAt  >  2:1150163/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                   TACGCCAATGTCGTTATCCAGCGGTGCACGGGTGAACTGATCGCGCAGCGGCGTCAGCAGTTGTTTTTTATCGCCAATCCACATCTGTGAAAGAAAGCCTGACTGGCGGTTAAATTGCCAACGCTTATTACCCAGCTCGATGCAAAAATCCATTTCGCTGGTGGTCAGATGCGGGATGGCGTGGGACGCGGCGGGGAGCGTCACACTGAGGTTTTCCGCCAGACGCCACTGCTGCCAGGCGCTGATGTGCCCGGCTTCTGACCAT  >  NC_000913/363918‑364182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑