Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	463,976	C→A	L14M (CTG→ATG)	ybaV →	helix‑hairpin‑helix 3 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	463,976	0	C	A	100.0%	266.2 / NA	75	L14M (CTG→ATG)	ybaV	helix‑hairpin‑helix 3 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (44/31);  total (44/31)

TTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAA  >  NC_000913/463841‑464110                                                                                                                                        |                                                                                                                                       tttCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGc                                                                                                                                    <  2:165221/140‑1 (MQ=255) tttCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGc                                                                                                                                    <  2:92359/140‑1 (MQ=255)      cgGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAAC‑AGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGc                                                                                                                              >  1:575321/1‑140 (MQ=255)         ccTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCg                                                                                                                            <  2:580235/140‑1 (MQ=255)                   ttCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCa                                                                                                                  >  2:20011/1‑140 (MQ=255)                   ttCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCa                                                                                                                  >  1:1378135/1‑140 (MQ=255)                        aaCATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAgcg                                                                                                             >  2:100266/1‑140 (MQ=255)                         aCATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAAC‑AGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAgcgcg                                                                                                           <  2:575321/140‑1 (MQ=255)                             tGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGa                                                                                                                          <  2:747325/122‑1 (MQ=255)                             tGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGa                                                                                                                          >  1:747325/1‑122 (MQ=255)                             tGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTg                                                                                                        >  2:127712/1‑140 (MQ=255)                             tGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTg                                                                                                        >  2:491116/1‑140 (MQ=255)                               ccATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTggc                                                                                                      >  1:366815/1‑140 (MQ=255)                               ccATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTggc                                                                                                      >  2:94755/1‑140 (MQ=255)                                  tttGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTggcggc                                                                                                   <  2:84814/140‑1 (MQ=255)                                   ttGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCa                                                                                                  >  2:186549/1‑140 (MQ=255)                                         aCTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCt                                                                                            >  1:709644/1‑140 (MQ=255)                                                     gCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCg                                                                                >  2:1297972/1‑140 (MQ=255)                                                     gCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCg                                                                                >  2:787040/1‑140 (MQ=255)                                                      ccGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGa                                                                               <  2:1319932/140‑1 (MQ=255)                                                         ttAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGAcgg                                                                            <  2:144828/140‑1 (MQ=255)                                                              gTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTa                                                                       <  1:153130/140‑1 (MQ=255)                                                                gATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAATACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGa                                                                     >  2:1302283/1‑140 (MQ=255)                                                                gATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGa                                                                     >  1:60233/1‑140 (MQ=255)                                                                 aTTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGaa                                                                    >  1:834886/1‑140 (MQ=255)                                                                 aTTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGaa                                                                    >  1:865169/1‑140 (MQ=255)                                                                 aTTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGaa                                                                    >  1:933838/1‑140 (MQ=255)                                                                    ccACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGa                                                                               >  1:602882/1‑126 (MQ=255)                                                                    ccACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGa                                                                               <  2:602882/126‑1 (MQ=255)                                                                     cACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCa                                                                <  2:366815/140‑1 (MQ=255)                                                                         gTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGc                                                            >  2:1298050/1‑140 (MQ=255)                                                                            aacaaacaAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGa                                                         <  1:491116/140‑1 (MQ=255)                                                                            aacaaacaAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGa                                                         <  1:100266/140‑1 (MQ=255)                                                                               aaacaaGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGc                                                      <  1:94755/140‑1 (MQ=255)                                                                                 acaaGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTc                                                    >  1:632457/1‑140 (MQ=255)                                                                                  caaGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGTCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTcc                                                   >  1:77126/1‑140 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGTAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 >  1:439885/1‑140 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 >  2:994116/1‑140 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 >  2:1076242/1‑140 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 <  1:1302283/140‑1 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 >  1:802919/1‑140 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 <  1:465739/140‑1 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 >  1:63759/1‑140 (MQ=255)                                                                                    aGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 >  1:573408/1‑140 (MQ=255)                                                                                         aaaaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTgcagca                                            <  2:865169/140‑1 (MQ=255)                                                                                         aaaaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTgcagca                                            <  1:510838/140‑1 (MQ=255)                                                                                         aaaaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTgcagca                                            <  2:573408/140‑1 (MQ=255)                                                                                           aaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCg                                                           >  1:524529/1‑123 (MQ=255)                                                                                           aaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCg                                                           <  2:524529/123‑1 (MQ=255)                                                                                           aaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 <  1:827386/133‑1 (MQ=255)                                                                                           aaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTg                                                 >  2:827386/1‑133 (MQ=255)                                                                                           aaCAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGcaca                                          >  2:719555/1‑140 (MQ=255)                                                                                                tATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGc                                                      >  1:956169/1‑123 (MQ=255)                                                                                                tATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGc                                                      <  2:956169/123‑1 (MQ=255)                                                                                                tATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTa                                     >  1:239084/1‑140 (MQ=255)                                                                                                 aTGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTaa                                    <  1:719555/140‑1 (MQ=255)                                                                                                 aTGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTaa                                    <  2:632457/140‑1 (MQ=255)                                                                                                   gAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAag                                  >  1:295251/1‑140 (MQ=255)                                                                                                     aaCACGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAagca                                >  1:1300699/1‑140 (MQ=255)                                                                                                      acacGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTATAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAagcag                               <  2:1300699/140‑1 (MQ=255)                                                                                                      acacGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAagcag                               <  1:1298050/140‑1 (MQ=255)                                                                                                      acacGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAagcag                               <  2:63759/140‑1 (MQ=255)                                                                                                        acGGAATTAAAGCACTGCTCATTACCCTGTTCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAgcagca                             >  1:176309/1‑140 (MQ=255)                                                                                                        acGGAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAgcagca                             >  1:1346822/1‑140 (MQ=255)                                                                                                          ggAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTgcagca                                            <  2:940140/123‑1 (MQ=255)                                                                                                          ggAATTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTgcagca                                            >  1:940140/1‑123 (MQ=255)                                                                                                            aaTTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTAc                         <  2:933838/140‑1 (MQ=255)                                                                                                            aaTTAAAGCACTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTAc                         <  2:709644/140‑1 (MQ=255)                                                                                                                      cTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGc               >  1:651423/1‑140 (MQ=255)                                                                                                                      cTGCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGc               >  2:713371/1‑140 (MQ=255)                                                                                                                        gCTCATTACCCTGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCa             >  2:675451/1‑140 (MQ=255)                                                                                                                                   tGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACgaagaa  >  1:480620/1‑140 (MQ=255)                                                                                                                                   tGTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACgaagaa  >  2:868610/1‑140 (MQ=255)                                                                                                                                    gTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGc               <  2:406322/126‑1 (MQ=255)                                                                                                                                    gTCCATGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGc               >  1:406322/1‑126 (MQ=255)                                                                                                                                        |                                                                                                                                       TTTCGCGGCCTTTTCCATTTCTGAACATTGCCATTTGTTTACTGTTTTCACTGCCGTTAAGGTGATTCCACTGTTAACAAACAAGGAGAAAACAGTATGAAACACGGAATTAAAGCACTGCTCATTACCCTGTCCCTGGCCTGTGCCGGAATGTCTCATAGCGCGCTGGCGGCAGCTTCTGTGGCGAAACCGACGGCGGTAGAAACCAAAGCGGAAGCTCCTGCAGCACAAAGTAAAGCAGCAGTACCGGCGAAAGCCAGTGACGAAGAA  >  NC_000913/463841‑464110

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑