Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	497,660	G→T	E162D (GAG→GAT)	adk →	adenylate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	497,660	0	G	T	100.0%	314.8 / NA	89	E162D (GAG→GAT)	adk	adenylate kinase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (43/46);  total (43/46)

TGACCGTATCGTCGGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCG  >  NC_000913/497525‑497793                                                                                                                                        |                                                                                                                                      tGACCGTATCGTCGGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACcg                                                                                                                                   >  2:1181152/1‑140 (MQ=255)  gACCGTATCGTCGGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACcgt                                                                                                                                  >  1:402412/1‑140 (MQ=255)  gACCGTATCGTCGGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACcgt                                                                                                                                  >  1:1288957/1‑140 (MQ=255)      gTATCGTCGGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACcgtacgt                                                                                                                              <  1:490110/140‑1 (MQ=255)        aTCGTCGGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTaa                                                                                                                            <  1:730924/140‑1 (MQ=255)          cgtcgGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAAc                                                                                                                          >  1:939296/1‑140 (MQ=255)           gtcggtcgCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACg                                                                                                                         <  2:1325646/140‑1 (MQ=255)             cggtcgCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTc                                                                                                                       <  1:481685/140‑1 (MQ=255)             cggtcgCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTc                                                                                                                       <  1:701019/140‑1 (MQ=255)                 cgccgcGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGt                                                                                                                   <  1:28003/140‑1 (MQ=255)                 cgccgcGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGt                                                                                                                   <  2:1288957/140‑1 (MQ=255)                    cgcgTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGa                                                                                                                >  2:1041633/1‑140 (MQ=255)                    cgcgTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGa                                                                                                                <  2:402412/140‑1 (MQ=255)                    cgcgTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGa                                                                                                                >  1:346735/1‑140 (MQ=255)                     gcgTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGaa                                                                                                               <  2:25348/140‑1 (MQ=255)                      cgTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAAt                                                                                                              <  2:346735/140‑1 (MQ=255)                        ttCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATAc                                                                                                            <  1:1388241/140‑1 (MQ=255)                           aTGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCAt                                                                                                         <  2:1065061/140‑1 (MQ=255)                           aTGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCAt                                                                                                         <  1:1041633/140‑1 (MQ=255)                           aTGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACAGTACGTAAACGTCTGGTTGAATACCAt                                                                                                         <  2:1378276/140‑1 (MQ=255)                                ccGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGAt                                                                                                    <  2:1296185/140‑1 (MQ=255)                                  gTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGa                                                                                                  >  2:331675/1‑140 (MQ=255)                                   tCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATAc                                                                                                            >  2:1310513/1‑129 (MQ=255)                                   tCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATAc                                                                                                            <  1:1310513/129‑1 (MQ=255)                                   tCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATggc                                                                                                 >  2:1208054/1‑138 (MQ=255)                                   tCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGAc                                                                                                 >  2:877640/1‑140 (MQ=255)                                   tCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGAc                                                                                                 >  2:358810/1‑140 (MQ=255)                                   tCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGAc                                                                                                 >  1:787221/1‑140 (MQ=255)                                   tCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGAc                                                                                                 >  1:640410/1‑140 (MQ=255)                                     tGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAg                                                                                               <  1:173828/140‑1 (MQ=255)                                       gTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCa                                                                                             <  2:65893/140‑1 (MQ=255)                                             tttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCAt                                                                                                         >  1:592311/1‑122 (MQ=255)                                             tttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCAt                                                                                                         <  2:592311/122‑1 (MQ=255)                                             tttATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTg                                                                                       >  1:792831/1‑140 (MQ=255)                                               tATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGAt                                                                                     >  2:444892/1‑140 (MQ=255)                                                 tCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGa                                                                                      >  1:86909/1‑137 (MQ=255)                                                 tCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGa                                                                                      <  2:86909/137‑1 (MQ=255)                                                  cACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCgg                                                                                  >  2:857503/1‑140 (MQ=255)                                                   aCGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGc                                                                                 <  2:348299/140‑1 (MQ=255)                                                     gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGcta                                                                               <  2:879122/140‑1 (MQ=255)                                                     gTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGcta                                                                               <  2:542229/140‑1 (MQ=255)                                                      ttAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGctac                                                                              >  2:930884/1‑140 (MQ=255)                                                      ttAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGctac                                                                              >  2:900415/1‑140 (MQ=255)                                                      ttAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGctac                                                                              >  1:1095898/1‑140 (MQ=255)                                                               aTCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCaaa                                                                     <  2:640410/140‑1 (MQ=255)                                                                 ccgccgAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAaaga                                                                   >  2:845261/1‑140 (MQ=255)                                                                   gccgAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAaagaag                                                                 <  2:965319/140‑1 (MQ=255)                                                                   gccgAAAGTAGAAGGCAAAGACGACGTTACCGGAGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAaagaag                                                                 >  2:368114/1‑140 (MQ=255)                                                                     cgAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAagaagca                                                               <  1:107810/140‑1 (MQ=255)                                                                        aaGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAagaagcagaa                                                            <  1:353985/140‑1 (MQ=255)                                                                        aaGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAagaagcagaa                                                            >  1:1222900/1‑140 (MQ=255)                                                                        aaGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAagaagcagaa                                                            <  1:877640/140‑1 (MQ=255)                                                                         aGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAagaagcagaag                                                           <  1:358810/140‑1 (MQ=255)                                                                            aGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAGCGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCgg                                                        <  2:950994/140‑1 (MQ=255)                                                                             gAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAACCGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCggg                                                       <  2:787221/140‑1 (MQ=255)                                                                             gAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCggg                                                       <  2:1095898/140‑1 (MQ=255)                                                                             gAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCggg                                                       <  1:900415/140‑1 (MQ=255)                                                                              aaGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGt                                                      <  1:958640/140‑1 (MQ=255)                                                                              aaGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAAAAGCGGGt                                                      <  1:444892/140‑1 (MQ=255)                                                                              aaGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGATGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGt                                                      >  1:1112419/1‑140 (MQ=255)                                                                                   aaaGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATAc                                                 >  2:1342266/1‑140 (MQ=255)                                                                                     aGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCa                                               >  2:268461/1‑140 (MQ=255)                                                                                     aGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCa                                               >  2:1141925/1‑140 (MQ=255)                                                                                      gacgacGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCaa                                              >  2:341714/1‑140 (MQ=255)                                                                                         gacgTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATa                                           <  2:1222900/140‑1 (MQ=255)                                                                                                ccGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGaaa                                    >  2:1201382/1‑140 (MQ=255)                                                                                                 cGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAg                                   <  2:939296/140‑1 (MQ=255)                                                                                                     gaagaaCTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGa                               <  1:1141925/140‑1 (MQ=255)                                                                                                     gaagaaCTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGa                               <  1:268461/140‑1 (MQ=255)                                                                                                      aagaaCTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGAc                              >  2:1399424/1‑140 (MQ=255)                                                                                                          aCTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCa                          >  1:458438/1‑140 (MQ=255)                                                                                                           cTGACTACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAGGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCAc                         >  2:1039844/1‑140 (MQ=255)                                                                                                              acttcccGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCaa                      >  1:1106821/5‑140 (MQ=255)                                                                                                                tACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGc                    >  1:94232/1‑140 (MQ=255)                                                                                                                tACCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATC‑GCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGcc                   <  2:787163/140‑1 (MQ=255)                                                                                                                 aCCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGcc                   <  2:979280/140‑1 (MQ=255)                                                                                                                 aCCCGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGcc                   <  1:341714/140‑1 (MQ=255)                                                                                                                   ccGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCgg                 >  1:1069986/1‑140 (MQ=255)                                                                                                                   ccGTAAAGATGATCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCgg                 >  2:1022089/1‑140 (MQ=255)                                                                                                                            tgatCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAg        >  1:1353076/1‑140 (MQ=255)                                                                                                                            tgatCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAg        >  1:301809/1‑140 (MQ=255)                                                                                                                             gatCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGt       >  2:431642/1‑140 (MQ=255)                                                                                                                             gatCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGt       <  2:792831/140‑1 (MQ=255)                                                                                                                             gatCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGt       >  2:1320063/1‑140 (MQ=255)                                                                                                                             gatCAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGt       >  1:246341/1‑140 (MQ=255)                                                                                                                                cAGGAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcg    >  1:1221485/1‑140 (MQ=255)                                                                                                                                  ggAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAGGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg  <  1:1039844/140‑1 (MQ=255)                                                                                                                                  ggAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg  <  2:1353076/140‑1 (MQ=255)                                                                                                                                  ggAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg  <  1:1201382/140‑1 (MQ=255)                                                                                                                                  ggAAGATACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTcgcg  <  1:744484/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                      TGACCGTATCGTCGGTCGCCGCGTTCATGCGCCGTCTGGTCGTGTTTATCACGTTAAATTCAATCCGCCGAAAGTAGAAGGCAAAGACGACGTTACCGGTGAAGAACTGACTACCCGTAAAGATGATCAGGAAGAGACCGTACGTAAACGTCTGGTTGAATACCATCAGATGACAGCACCGCTGATCGGCTACTACTCCAAAGAAGCAGAAGCGGGTAATACCAAATACGCGAAAGTTGACGGCACCAAGCCGGTTGCTGAAGTTCGCG  >  NC_000913/497525‑497793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑