Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	946,335	C→A	T155T (ACC→ACA)	ycaD →	putative transporter YcaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	946,335	0	C	A	100.0%	215.3 / NA	61	T155T (ACC→ACA)	ycaD	putative transporter YcaD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (36/25);  total (36/25)

TGATTTGGGTGGTTGTTGAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCC  >  NC_000913/946199‑946468                                                                                                                                         |                                                                                                                                      tGATTTGGGTGGTTGTTGAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACagag                                                                                                                                    <  1:1000268/140‑1 (MQ=255) tGATTTGGGTGGTTGTTGAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACagag                                                                                                                                    <  2:470248/140‑1 (MQ=255)        ggtggtTGTTGAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGt                                                                                                                             >  2:1446610/1‑140 (MQ=255)            gttgttGAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGt                                                                                                                         >  1:769903/1‑140 (MQ=255)             ttgttgAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTa                                                                                                                        <  1:690941/140‑1 (MQ=255)              tgttgAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTAt                                                                                                                       >  1:1048378/1‑140 (MQ=255)                  gagaGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGcc                                                                                                                   <  2:391836/140‑1 (MQ=255)                   agagCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCg                                                                                                                  <  1:1304760/140‑1 (MQ=255)                     agCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTg                                                                                                                <  2:704199/140‑1 (MQ=255)                       cgcgcTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTggg                                                                                                              >  1:291489/1‑140 (MQ=255)                       cgcgcTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTggg                                                                                                              >  1:532152/1‑140 (MQ=255)                         cgcTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGtt                                                                                                            >  1:595263/1‑140 (MQ=255)                          gcTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTa                                                                                                           >  1:150774/1‑140 (MQ=255)                          gcTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTa                                                                                                           <  1:187062/140‑1 (MQ=255)                          gcTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTa                                                                                                           <  2:1342496/140‑1 (MQ=255)                                    aGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGAc                                                                                                 >  1:284045/1‑140 (MQ=255)                                    aGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGAc                                                                                                 >  1:979235/1‑140 (MQ=255)                                     gTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACg                                                                                                <  2:546073/140‑1 (MQ=255)                                      tGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGt                                                                                               <  1:182647/140‑1 (MQ=255)                                        ggACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTg                                                                                             >  1:1025747/1‑140 (MQ=255)                                        ggACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTg                                                                                             <  1:501292/140‑1 (MQ=255)                                        ggACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGACGTGGGTTACAGGTTTGACGTTg                                                                                             >  1:882838/1‑140 (MQ=255)                                           cGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCa                                                                                          <  1:1446610/140‑1 (MQ=255)                                             tCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAgg                                                                                        >  1:612557/1‑140 (MQ=255)                                             tCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAgg                                                                                        <  2:769435/140‑1 (MQ=255)                                                cGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGAt                                                                                     >  2:433447/1‑140 (MQ=255)                                                     ccGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTAc                                                                                <  2:595263/140‑1 (MQ=255)                                                        tGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGc                                                                             >  1:602559/1‑140 (MQ=255)                                                        tGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGc                                                                             <  1:1093463/140‑1 (MQ=255)                                                        tGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGc                                                                             <  2:532152/140‑1 (MQ=255)                                                          ggCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCtg                                                                           <  2:1303111/140‑1 (MQ=255)                                                          ggCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCtg                                                                           >  2:486374/1‑140 (MQ=255)                                                           gCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCtgt                                                                          >  2:862683/1‑140 (MQ=255)                                                           gCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCtgt                                                                          <  2:451719/140‑1 (MQ=255)                                                               ttgcttgcTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTgttgtt                                                                      <  2:979235/140‑1 (MQ=255)                                                               ttgcttgcTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTgttgtt                                                                      <  2:284045/140‑1 (MQ=255)                                                                tgcttgctGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGttt                                                                     >  2:971151/1‑140 (MQ=255)                                                                  cttgctGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTAc                                                                   >  2:87841/1‑140 (MQ=255)                                                                        gcGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCgtgt                                                             <  2:335613/140‑1 (MQ=255)                                                                           tataTGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTa                                                                    >  1:722106/1‑130 (MQ=255)                                                                           tataTGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTa                                                                    <  2:722106/130‑1 (MQ=255)                                                                           tataTGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCt                                                          >  2:643304/1‑140 (MQ=255)                                                                                 atgGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATca                                                    >  2:671342/1‑140 (MQ=255)                                                                                   ggTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATcagc                                                  >  2:667059/1‑140 (MQ=255)                                                                                   ggTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATcagc                                                  <  2:291489/140‑1 (MQ=255)                                                                                   ggTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATcagc                                                  >  1:254515/1‑140 (MQ=255)                                                                                   ggTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATcagc                                                  <  1:971151/140‑1 (MQ=255)                                                                                   ggTTTATTAAGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGATTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTTGTGAATgagc                                                  >  2:227232/1‑140 (MQ=255)                                                                                       tattaCGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGc                                              >  2:135396/1‑140 (MQ=255)                                                                                        attaCGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCt                                             <  1:667059/140‑1 (MQ=255)                                                                                          taCGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGa                                           <  2:150774/140‑1 (MQ=255)                                                                                                ggAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCa                                     >  1:1134310/1‑140 (MQ=255)                                                                                                   aCGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGa                                  >  2:217752/1‑140 (MQ=255)                                                                                                    cGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGAt                                 <  2:254515/140‑1 (MQ=255)                                                                                                      tttttAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTc                               >  2:131550/1‑140 (MQ=255)                                                                                                       ttttAGGCCAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCg                              >  1:185260/1‑140 (MQ=255)                                                                                                               cAGTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAAt                      >  2:209865/1‑140 (MQ=255)                                                                                                                 gTTACTGGTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTa                    >  2:1334715/1‑140 (MQ=255)                                                                                                                       ggTCAGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCaa              <  2:1025747/140‑1 (MQ=255)                                                                                                                           aGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCt          >  1:495792/1‑140 (MQ=255)                                                                                                                           aGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCt          >  2:461066/1‑140 (MQ=255)                                                                                                                           aGCAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCt          >  2:912549/1‑140 (MQ=255)                                                                                                                             cAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTaa        >  1:988678/1‑140 (MQ=255)                                                                                                                             cAAAGTTTCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTaa        >  2:444210/1‑140 (MQ=255)                                                                                                                                  tttCAACAGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACG‑TGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTcc  >  2:425680/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TGATTTGGGTGGTTGTTGAGAGCGCGCTGATGTGCAGTGGGACGTCACGTAACCGTGGGCGTTTGCTTGCTGCGTATATGATGGTTTATTACGTGGGAACGTTTTTAGGCCAGTTACTGGTCAGCAAAGTTTCAACCGAGCTGATGTCCGTATTGCCGTGGGTTACAGGTTTGACGTTGGCAGGGATCTTACCGCTGTTGTTTACGCGTGTGCTGAATCAGCAGGCTGAAAACCATGATTCGACGTCAATTACGTCAATGCTAAAACTCC  >  NC_000913/946199‑946468

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑