Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,744,065	C→A	V203V (GTC→GTA)	mdtK →	multidrug efflux pump MdtK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,744,065	0	C	A	100.0%	50.3 / NA	16	V203V (GTC→GTA)	mdtK	multidrug efflux pump MdtK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (3/13);  total (3/13)

TGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGAT  >  NC_000913/1743938‑1744190                                                                                                                                |                                                                                                                               tGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGcc                                                                                                                    <  2:392205/140‑1 (MQ=255)    tAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAGCTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATg                                                                                                                 <  1:185593/140‑1 (MQ=255)        gggCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGttt                                                                                                             <  2:210647/140‑1 (MQ=255)           cTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCtt                                                                                                          >  1:545373/1‑140 (MQ=255)           cTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCt                                                                                                           >  2:73253/1‑139 (MQ=255)           cTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCt                                                                                                           <  1:73253/139‑1 (MQ=255)                   gCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTaaa                                                                                                  <  2:310450/140‑1 (MQ=255)                                  tCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATg                                                                                   <  2:545373/140‑1 (MQ=255)                                  tCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGGAATGTTTCTTTCCATGGGTTCTTACATTTAACGCGCCCGCTCCATg                                                                                   >  2:107047/1‑140 (MQ=255)                                   cccGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGGAATGTTTCTTTTCATGGGTTTTTTCATTTAACGCGCCCGCGCCCTgc                                                                                  >  2:234194/1‑140 (MQ=255)                                    ccGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATgcg                                                                                 <  1:26425/140‑1 (MQ=255)                                                       tttatGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGGAATGGTTCTTGCCATGGGTTCTTACATTTAACGCGCCCGCTCCATGCGCGGTATTCGTAA‑CGaaaaa                                                             >  2:639688/1‑140 (MQ=255)                                                          atGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGc                                                          <  1:180925/140‑1 (MQ=255)                                                           tGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATTTTCCTTTCCCTGGGTTCTTACATTAAAAGCGCCCCCCCCATGCGCGGTATTCGTAACCGAAAAAgca                                                          >  2:180925/1‑138 (MQ=255)                                                            ggTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAAAGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCAc                                                        >  1:259935/1‑140 (MQ=255)                                                             gTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGGATTGGGGAATGTTCCTTGCCGTGGTTTCTTTCATTAAACCCGCCCGCGCCATGCGCGGTATTTGTAA‑CGAAAAAAGTAcc                                                       >  2:307802/1‑140 (MQ=255)                                                                aTTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCa                                                    <  1:234194/140‑1 (MQ=255)                                                                            cTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGGGTATTTGGGAATGTTTCTTGCCCTGGGTTCTTATATTAAACGCGCCCGCTCCATTGGCGATATTTGTAA‑AGAAAAAAGAACCCCAAAAACCGATccc                                        >  2:484458/1‑139 (MQ=255)                                                                             tGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTg                                       <  1:443445/140‑1 (MQ=255)                                                                             tGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTg                                       <  2:259935/140‑1 (MQ=255)                                                                               aGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGGATTTGGGAATGTTTCTTGGCCTGGGTTCTTACATTAAACGCGCCCGCCCCAAGGGCGATATTCGGAA‑AGAAAAAGGCACCGCAAAAACCGATCCTGCg                                     >  2:443445/1‑140 (MQ=255)                                                                                 cTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGGGGATTGGGGAATGTTTCTTTCCATGGGTTTTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTTA‑CGAAAAAAGCACCGCAAAACCCCATCCTGCggg                                   >  2:253996/1‑139 (MQ=255)                                                                                 cTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGGGGATTGGGGAATGTTTCTTGCCATGGTTTTTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTGCGGt                                   >  1:231494/1‑140 (MQ=255)                                                                                    ggTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGGAAAGTTCCTTGCCATGGGTTCTTTCATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAAGCACCGCCAAACCCCATCCTGCGGTTAt                                >  2:635336/1‑140 (MQ=255)                                                                                                        ggcTACTGCGGCGGGGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTTATTCAACTCg            >  1:332645/1‑140 (MQ=255)                                                                                                            aCTGCGGCGGTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGttt        <  1:253996/140‑1 (MQ=255)                                                                                                                  gcggTGTATTGGGTAATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGAt  <  2:151852/140‑1 (MQ=255)                                                                                                                                |                                                                                                                               TGGTAATGGGCTTTATCGGCCTGCTGGTGAACATCCCGGTGAACTATATCTTTATTTATGGTCATTTCGGTATGCCTGAGCTCGGTGGCGTTGGTTGTGGCGTGGCTACTGCGGCGGTGTATTGGGTCATGTTCCTTGCCATGGTTTCTTACATTAAACGCGCCCGCTCCATGCGCGATATTCGTAA‑CGAAAAAGGCACCGCAAAACCCGATCCTGCGGTTATGAAACGACTGATTCAACTCGGTTTGCCGAT  >  NC_000913/1743938‑1744190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑