Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,063,387	G→T	R134L (CGA→CTA)	argK →	methylmalonyl‑CoA mutase‑interacting GTPase YgfD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,063,387	0	G	T	100.0%	147.1 / NA	42	R134L (CGA→CTA)	argK	methylmalonyl‑CoA mutase‑interacting GTPase YgfD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (25/17);  total (25/17)

TGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGT  >  NC_000913/3063250‑3063520                                                                                                                                          |                                                                                                                                      tGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCCGGCGCGTGCCGAAGCGGCGGTTATTCGCCCGGTACCATCCTCCGGTCATCTTGGCGGTGCCAGTCagctag                                                                                                                                     >  1:552938/1‑140 (MQ=255)           cccAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAAtt                                                                                                                           >  1:686997/1‑140 (MQ=255)               gcagcCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATg                                                                                                                       <  2:543229/140‑1 (MQ=255)                   cccGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGAGAATTAATGCTGt                                                                                                                   >  2:74798/1‑140 (MQ=255)                      ggTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTAt                                                                                                                >  2:756693/1‑140 (MQ=255)                        tCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGc                                                                                                              >  2:38866/1‑140 (MQ=255)                             ggCGGTAGCATTCTCGGGGGTAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGGGCCAGTCAGCTAGCGCGGGGATTAATGCTGTTATGCGAagc                                                                                                         >  2:492775/1‑140 (MQ=255)                               cGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagcag                                                                                                       <  1:38866/140‑1 (MQ=255)                               cGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagcag                                                                                                       <  1:756693/140‑1 (MQ=255)                                  tAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagca                                                                                                        >  2:651343/1‑136 (MQ=255)                                  tAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAagca                                                                                                        <  1:651343/136‑1 (MQ=255)                                  tAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGGTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCgg                                                                                                    >  2:390341/1‑140 (MQ=255)                                      aTTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGTGAAGCAGCGGGTTa                                                                                                <  2:686997/140‑1 (MQ=255)                                      aTTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGAGAATTAATGCTGTTATGCGAAGCAGCGGGTTa                                                                                                <  1:74798/140‑1 (MQ=255)                                                         gCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaa                                                                             <  1:655123/140‑1 (MQ=255)                                                          cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:655484/1‑140 (MQ=255)                                                          cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:694377/1‑140 (MQ=255)                                                          cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:541372/1‑140 (MQ=255)                                                          cATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGaaa                                                                            >  1:476838/1‑140 (MQ=255)                                                              aatgaCCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGt                                                                        <  1:177077/140‑1 (MQ=255)                                                                       gcgcGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCgg                                                               >  2:613156/1‑140 (MQ=255)                                                                        cgcgTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCggg                                                              <  1:293919/140‑1 (MQ=255)                                                                            tGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGt                                                          >  1:687183/1‑140 (MQ=255)                                                                                  aGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGaaa                                                    >  1:96407/1‑140 (MQ=255)                                                                                  aGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGaaa                                                    <  2:21773/140‑1 (MQ=255)                                                                                       cgTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGaa                                               <  1:613156/140‑1 (MQ=255)                                                                                              tCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGccc                                        <  2:96407/140‑1 (MQ=255)                                                                                              tCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGccc                                        <  2:655484/140‑1 (MQ=255)                                                                                                   cGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAtg                                   <  1:411043/140‑1 (MQ=255)                                                                                                      tACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAtggtg                                >  2:154385/1‑140 (MQ=255)                                                                                                                cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGa                              >  2:397305/1‑132 (MQ=255)                                                                                                                cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGa                              <  1:397305/132‑1 (MQ=255)                                                                                                                cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  1:195741/1‑140 (MQ=255)                                                                                                                cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  2:465283/1‑140 (MQ=255)                                                                                                                cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  2:723910/1‑140 (MQ=255)                                                                                                                cGGTCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTa                      >  2:269347/1‑140 (MQ=255)                                                                                                                   tCATCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTAtct                   >  2:698099/1‑140 (MQ=255)                                                                                                                     aTCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCg                 >  2:662580/1‑140 (MQ=255)                                                                                                                      tCTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGt                >  1:755059/1‑140 (MQ=255)                                                                                                                       cTGGGCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGtt               >  1:520633/1‑140 (MQ=255)                                                                                                                          ggCGGTGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCa            >  2:451239/1‑140 (MQ=255)                                                                                                                               tGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGt                >  2:132658/1‑131 (MQ=255)                                                                                                                               tGCCAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGt                <  1:132658/131‑1 (MQ=255)                                                                                                                                 ccAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGcc     >  1:544838/1‑140 (MQ=255)                                                                                                                                 ccAGTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGcc     >  2:712409/1‑140 (MQ=255)                                                                                                                                    gTCAGCTAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGt  <  2:614072/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                      TGCGGTCGATCCCAGCAGCCCGGTCACTGGCGGTAGCATTCTCGGGGATAAAACCCGCATGAATGACCTGGCGCGTGCCGAAGCGGCGTTTATTCGCCCGGTACCATCCTCCGGTCATCTGGGCGGTGCCAGTCAGCGAGCGCGGGAATTAATGCTGTTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGT  >  NC_000913/3063250‑3063520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑