Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,192,822	G→A	W205* (TGG→TGA)	yqiJ →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,192,822	0	G	A	100.0%	122.1 / NA	36	W205* (TGG→TGA)	yqiJ	putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/22);  total (14/22)

ACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTAT  >  NC_000913/3192691‑3192937                                                                                                                                    |                                                                                                                    aCCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAat                                                                                                             <  2:170843/140‑1 (MQ=255)             ggCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATttat                                                                                                 <  2:431139/140‑1 (MQ=255)                        ttattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAgg                                                                                      <  2:226536/140‑1 (MQ=255)                         tattTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGa                                                                                     >  2:581445/1‑140 (MQ=255)                              actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAAttt                                                                                >  1:281579/1‑140 (MQ=255)                              actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAAttt                                                                                >  2:286847/1‑140 (MQ=255)                              actactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAAttt                                                                                >  1:435546/1‑140 (MQ=255)                                tactGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaa                                                                              <  1:286847/140‑1 (MQ=255)                                  ctGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaata                                                                            <  2:281579/140‑1 (MQ=255)                                  ctGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTtaata                                                                            <  1:634820/140‑1 (MQ=255)                                     gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                                                         >  1:294227/1‑140 (MQ=255)                                     gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                                                         >  1:704673/1‑140 (MQ=255)                                     gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATg                                                                         >  1:698535/1‑140 (MQ=255)                                     gAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGCAAATTTAATAATg                                                                         >  2:394303/1‑140 (MQ=255)                                       gCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAgg                                                                                      >  1:22067/1‑125 (MQ=255)                                       gCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAgg                                                                                      <  2:22067/125‑1 (MQ=255)                                       gCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGga                                                                       <  1:333446/140‑1 (MQ=255)                                        ccTGAAGAAGAAAAAATTTT‑AATAAAAGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatg                                                                     <  1:391394/140‑1 (MQ=255)                                           gaagaaGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGgatgat                                                                   <  1:215156/140‑1 (MQ=255)                                                       aTTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCt                                                       <  1:581445/140‑1 (MQ=255)                                                        ttttCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATGCtg                                                      <  2:698535/140‑1 (MQ=255)                                                            cACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGcc                                                  <  1:516078/140‑1 (MQ=255)                                                            cACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGcc                                                  <  1:467866/140‑1 (MQ=255)                                                              cTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcct                                                <  2:294227/140‑1 (MQ=255)                                                                aaaGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCcctcc                                              <  2:516438/140‑1 (MQ=255)                                                                         aaaGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGttt                                     >  1:500794/1‑140 (MQ=255)                                                                                tAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCaa                              >  2:755475/1‑140 (MQ=255)                                                                                       ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatta                           >  2:643859/1‑136 (MQ=255)                                                                                       ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAatta                           <  1:643859/136‑1 (MQ=255)                                                                                       ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGc                       <  2:421203/140‑1 (MQ=255)                                                                                       ttGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGc                       <  1:295923/140‑1 (MQ=255)                                                                                         gCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCg                     >  2:422419/1‑140 (MQ=255)                                                                                             aCTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATg                 <  1:422419/140‑1 (MQ=255)                                                                                                 tCTGCAACGCGATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCAtt             <  2:704673/140‑1 (MQ=255)                                                                                                         gcgATATTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTttat     >  1:26678/1‑140 (MQ=255)                                                                                                            atatTTGGCGGAAAATAATCCCTGACCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTtattat  <  2:26678/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                    ACCGATCAATTTGGCCAAATACATTATTTACTACTGGAGCCTGAAGAAGGAAAAATTTTCACTAAAGGAGACAAAGTGTTAATTATTTGCCGACTTTCTGCAACGCGATATTTGGCGGAAAATAATCCCTGGCCCCAAATATTGTAATTTATTAATCAAAAGGAAATTTAATAATGGATGATATTGTTAATTCTGTGCCCTCCTGGATGTTTACCGCAATTATTGCCGTATGCATTCTGTTTATTAT  >  NC_000913/3192691‑3192937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑