Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	273,156	C→A	P104T (CCC→ACC)	ykfC →	CP4‑6 prophage; PF00078 domain‑containing protein YkfC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	273,156	0	C	A	100.0%	227.5 / NA	67	P104T (CCC→ACC)	ykfC	CP4‑6 prophage; PF00078 domain‑containing protein YkfC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (34/33);  total (34/33)

AACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACGGTGA  >  NC_000913/273020‑273288                                                                                                                                         |                                                                                                                                     aacaaTGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACcg                                                                                                                                   <  1:372352/140‑1 (MQ=255) aacaaTGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACcg                                                                                                                                   >  2:763048/1‑140 (MQ=255)      tGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTg                                                                                                                              <  1:202065/140‑1 (MQ=255)      tGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTg                                                                                                                              <  1:763048/140‑1 (MQ=255)      tGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTg                                                                                                                              <  1:495443/140‑1 (MQ=255)      tGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTg                                                                                                                              <  1:389646/140‑1 (MQ=255)         tACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCgg                                                                                                                           >  1:487404/1‑140 (MQ=255)           cAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGa                                                                                                                         >  1:726937/1‑140 (MQ=255)           cAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGa                                                                                                                         >  1:663285/1‑140 (MQ=255)           cAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGa                                                                                                                         <  2:62073/140‑1 (MQ=255)           cAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGa                                                                                                                         >  1:437585/1‑140 (MQ=255)                   aCTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTg                                                                                                                 >  2:125224/1‑140 (MQ=255)                   aCTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTg                                                                                                                 >  1:284982/1‑140 (MQ=255)                   aCTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTg                                                                                                                 >  2:525341/1‑140 (MQ=255)                   aCTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTg                                                                                                                 >  2:37478/1‑140 (MQ=255)                       gCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCa                                                                                                             >  2:708915/1‑140 (MQ=255)                        cTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAg                                                                                                            >  1:39417/1‑140 (MQ=255)                        cTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAg                                                                                                            >  2:99649/1‑140 (MQ=255)                        cTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAg                                                                                                            <  2:241384/140‑1 (MQ=255)                        cTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAg                                                                                                            >  1:241384/1‑140 (MQ=255)                        cTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAg                                                                                                            >  2:595213/1‑140 (MQ=255)                         tGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGc                                                                                                           <  2:39417/140‑1 (MQ=255)                         tGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGc                                                                                                           <  1:595213/140‑1 (MQ=255)                          gTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCg                                                                                                          >  1:680902/1‑140 (MQ=255)                              aGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGcc                                                                                                      <  1:555831/140‑1 (MQ=255)                                       tCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATg                                                                                             <  2:726937/140‑1 (MQ=255)                                        ccTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATgg                                                                                            >  2:320040/1‑140 (MQ=255)                                           cAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGa                                                                                         <  1:406957/140‑1 (MQ=255)                                            aGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGAt                                                                                        >  1:325245/1‑140 (MQ=255)                                               gATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGa                                                                                     <  1:320040/140‑1 (MQ=255)                                                   aaTTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCg                                                                                 >  2:539436/1‑140 (MQ=255)                                                      tACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGata                                                                              <  2:284982/140‑1 (MQ=255)                                                        ctctCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATg                                                                            >  2:209284/1‑140 (MQ=255)                                                        ctctCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTCCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATg                                                                            >  2:712015/1‑140 (MQ=255)                                                             aGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGgaga                                                                       <  2:437585/140‑1 (MQ=255)                                                             aGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGgaga                                                                       <  2:663285/140‑1 (MQ=255)                                                              ggCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGagag                                                                      <  1:209284/140‑1 (MQ=255)                                                               gCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGagagg                                                                     >  1:480427/1‑139 (MQ=255)                                                               gCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGagagg                                                                     >  1:619470/1‑139 (MQ=255)                                                               gCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGt                                                                     >  2:766756/1‑140 (MQ=255)                                                                   cTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGAtt                                                                 >  2:691884/1‑140 (MQ=255)                                                                         gCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATa                                                           >  1:270575/1‑140 (MQ=255)                                                                         gCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATa                                                           >  1:491488/1‑140 (MQ=255)                                                                         gCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATa                                                           >  1:678627/1‑140 (MQ=255)                                                                           ccTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACg                                                         <  1:729390/140‑1 (MQ=255)                                                                           ccTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACg                                                         <  1:37478/140‑1 (MQ=255)                                                                           ccTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCACCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACg                                                         <  2:72550/140‑1 (MQ=255)                                                                              tGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGctc                                                      >  1:424239/1‑140 (MQ=255)                                                                              tGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGctc                                                      >  2:319815/1‑140 (MQ=255)                                                                                   gCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCata                                                 <  2:491488/140‑1 (MQ=255)                                                                                   gCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTCCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCata                                                 <  1:712015/140‑1 (MQ=255)                                                                                    ccAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATa                                                           >  1:686511/1‑129 (MQ=255)                                                                                    ccAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATa                                                           <  2:686511/129‑1 (MQ=255)                                                                                                    cccTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAgc                                <  2:424239/140‑1 (MQ=255)                                                                                                    cccTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAgc                                <  1:539436/140‑1 (MQ=255)                                                                                                     ccTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAgcg                               <  2:487404/140‑1 (MQ=255)                                                                                                     ccTAAAAGCAACGGCAAACTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAgcg                               <  1:525341/140‑1 (MQ=255)                                                                                                      cTAAAAGCAACGGCAAACTGCGACCACTGGGTAACACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTGATATGGCTTCCGGCCTGAgcgc                              >  2:574266/1‑140 (MQ=255)                                                                                                                       cTGCGACCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGAt             >  2:126286/1‑140 (MQ=255)                                                                                                                            aCCACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCa        >  1:526671/1‑140 (MQ=255)                                                                                                                             ccACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCAc       <  2:680902/140‑1 (MQ=255)                                                                                                                             ccACTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCAc       <  2:270575/140‑1 (MQ=255)                                                                                                                               aCTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACgg     <  1:708915/140‑1 (MQ=255)                                                                                                                               aCTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACgg     <  2:325245/140‑1 (MQ=255)                                                                                                                               aCTGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACgg     <  1:319815/140‑1 (MQ=255)                                                                                                                                 tGGGTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACGGTg   >  2:294536/1‑140 (MQ=255)                                                                                                                                  gggTATCACCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACGGTGa  <  1:691884/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                     AACAATGCTACAGGCCAGACTGGCTGTTGAGCTGCAAATCCTCAGGGATGAATTACTCTCAGGCCACTACCAGCCCTTGCCCGCCAGACGGGTTTACATCCCTAAAAGCAACGGCAAACTGCGACCACTGGGTATCCCCGCGTTGCGGGATCGTATTGTTCAGCGGGCCATGCTGATGGCGATGGAGCCGATATGGGAGAGTGATTTTCATACGCTCTCATATGGCTTCCGGCCTGAGCGCAGTGTCCACCACGCGATCCGCACGGTGA  >  NC_000913/273020‑273288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑