Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,682,266	C→A	A394A (GCG→GCT)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,682,266	0	C	A	100.0%	209.7 / NA	59	A394A (GCG→GCT)	dctA	C4 dicarboxylate/orotate:H(+) symporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (31/28);  total (31/28)

GGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCAC  >  NC_000913/3682130‑3682402                                                                                                                                         |                                                                                                                                         ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCt                                                                                                                                       >  2:9682/1‑140 (MQ=255) ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCt                                                                                                                                       >  2:675005/1‑140 (MQ=255) ggggAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCt                                                                                                                                       >  2:300702/1‑140 (MQ=255)       gggAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCg                                                                                                                                 >  2:257186/1‑140 (MQ=255)           gTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTAc                                                                                                                             >  2:351199/1‑140 (MQ=255)              cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:610431/140‑1 (MQ=255)              cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:701713/140‑1 (MQ=255)              cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:300702/140‑1 (MQ=255)              cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  2:251617/140‑1 (MQ=255)              cGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:351199/140‑1 (MQ=255)               gggCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGAc                                                                                                                         >  2:765965/1‑140 (MQ=255)                   aTAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTa                                                                                                                              <  1:326552/131‑1 (MQ=255)                   aTAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTa                                                                                                                              >  2:326552/1‑131 (MQ=255)                   aTAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAgg                                                                                                                     >  2:325330/1‑140 (MQ=255)                        tgatgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGt                                                                                                                <  1:601347/140‑1 (MQ=255)                           tgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          >  2:599639/1‑127 (MQ=255)                           tgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGa                                                                                                                          <  1:599639/127‑1 (MQ=255)                           tgaGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAg                                                                                                             >  2:124068/1‑140 (MQ=255)                             agaTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAgcg                                                                                                           <  2:337261/140‑1 (MQ=255)                               aTTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCa                                                                                                         <  1:9682/140‑1 (MQ=255)                                 tAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACg                                                                                                       <  1:257186/140‑1 (MQ=255)                                      ggATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  <  2:752693/140‑1 (MQ=255)                                      ggATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  <  2:511066/140‑1 (MQ=255)                                       gATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTc                                                                                                 >  1:270132/1‑140 (MQ=255)                                       gATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTc                                                                                                 <  2:211403/140‑1 (MQ=255)                                         tAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATAGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  <  1:520405/137‑1 (MQ=255)                                         tAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATAGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCtt                                                                                                  >  2:520405/1‑137 (MQ=255)                                                tGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATaaa                                                                                        >  1:200742/1‑140 (MQ=255)                                                  cGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATaaa                                                                                        <  2:8939/138‑1 (MQ=255)                                                  cGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATaaa                                                                                        >  1:8939/1‑138 (MQ=255)                                                      ttGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTc                                                                                  <  1:325330/140‑1 (MQ=255)                                                        gCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGa                                                                                <  1:124068/140‑1 (MQ=255)                                                                 cgcACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGa                                                                       >  1:164833/1‑140 (MQ=255)                                                                                cacaTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCg                                                        >  2:421388/1‑140 (MQ=255)                                                                                 acaTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGc                                                       <  2:200742/140‑1 (MQ=255)                                                                                  caTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGa                                                            >  1:402156/1‑134 (MQ=255)                                                                                  caTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGa                                                            <  2:402156/134‑1 (MQ=255)                                                                                   aTCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTa                                                     <  2:110812/140‑1 (MQ=255)                                                                                     cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGAcc                                                          >  1:11768/1‑133 (MQ=255)                                                                                     cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGAcc                                                          <  2:11768/133‑1 (MQ=255)                                                                                     cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  1:466336/1‑140 (MQ=255)                                                                                     cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  2:650105/1‑140 (MQ=255)                                                                                     cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  2:577834/1‑140 (MQ=255)                                                                                     cGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAcc                                                   >  1:760512/1‑140 (MQ=255)                                                                                                ttGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGcc                                        <  2:164833/140‑1 (MQ=255)                                                                                                ttGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGcc                                        <  2:270132/140‑1 (MQ=255)                                                                                                                tCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                        >  1:708388/1‑140 (MQ=255)                                                                                                                tCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                        >  1:701561/1‑140 (MQ=255)                                                                                                                tCACCCACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                        >  1:124925/1‑140 (MQ=255)                                                                                                                   cccACTTAGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTcgcc                     <  2:124925/140‑1 (MQ=255)                                                                                                                          aGCAACGACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCa              >  1:550835/1‑140 (MQ=255)                                                                                                                                gACAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaa        <  2:760512/140‑1 (MQ=255)                                                                                                                                 aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  2:411351/1‑140 (MQ=255)                                                                                                                                 aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  2:392764/1‑140 (MQ=255)                                                                                                                                 aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  2:51249/1‑140 (MQ=255)                                                                                                                                 aCAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATaaa       >  1:506760/1‑140 (MQ=255)                                                                                                                                  cAATGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAg      >  2:707757/1‑140 (MQ=255)                                                                                                                                   aaTGGTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGc     <  1:577834/140‑1 (MQ=255)                                                                                                                                      ggTAGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCAc  <  1:675005/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         GGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCAC  >  NC_000913/3682130‑3682402

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑