Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,619,014	C→A	F262L (TTC→TTA)	deoA →	thymidine phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,619,014	0	C	A	97.2%	119.7 / ‑3.4	36	F262L (TTC→TTA)	deoA	thymidine phosphorylase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (19/16);  minor base T (1/0);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.63e-01

TTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAAC  >  NC_000913/4618887‑4619146                                                                                                                                |                                                                                                                                     ttGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGaa                                                                                                                          >  2:523140/1‑140 (MQ=255)    ccGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAatat                                                                                                                       <  1:475916/140‑1 (MQ=255)            aTTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGCACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAAccc                                                                                                               >  2:712677/1‑140 (MQ=255)               gttgGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCcgcg                                                                                                            <  2:196181/140‑1 (MQ=255)                     gTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGtt                                                                                                      <  1:623860/140‑1 (MQ=255)                      tGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGttt                                                                                                     <  2:665332/140‑1 (MQ=255)                      tGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGttt                                                                                                     >  2:629157/1‑140 (MQ=255)                       ggCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCg                                                                                                                     <  2:362896/123‑1 (MQ=255)                       ggCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCg                                                                                                                     >  1:362896/1‑123 (MQ=255)                       ggCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGt                                                                                                                    >  2:768539/1‑124 (MQ=255)                       ggCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGt                                                                                                                    <  1:768539/124‑1 (MQ=255)                        gCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGa                                                                                                   >  2:482327/1‑140 (MQ=255)                                   tGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATgg                                                                                        >  1:755940/1‑140 (MQ=255)                                     gCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGgcg                                                                                      <  2:117126/140‑1 (MQ=255)                                      cGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGgcgc                                                                                     <  1:482327/140‑1 (MQ=255)                                      cGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGgcgc                                                                                     <  2:755940/140‑1 (MQ=255)                                               caccGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTgg                                                                            >  2:569433/1‑140 (MQ=255)                                                      ctgctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCt                                                                     <  1:629157/140‑1 (MQ=255)                                                         ctCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGAt                                                                  >  2:707928/1‑140 (MQ=255)                                                                 cATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCa                                                          <  1:569433/140‑1 (MQ=255)                                                                      aTCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTg                                                     >  1:234975/1‑140 (MQ=255)                                                                       tCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTgg                                                    <  2:234975/140‑1 (MQ=255)                                                                          ggTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGa                                                 >  2:625110/1‑140 (MQ=255)                                                                                 gCCTCCAGTGCAGGTAACGCGGTTGTAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGa                                          >  2:255329/1‑140 (MQ=255)                                                                                 gCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGa                                          >  1:150669/1‑140 (MQ=255)                                                                                           cAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTTCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcg                                >  2:685900/1‑140 (MQ=255)                                                                                              gTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcgcgc                             >  1:452015/1‑140 (MQ=255)                                                                                                aaCGCGGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgcgcgcgc                           >  2:740658/1‑140 (MQ=255)                                                                                                   gcgGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgc                                 >  2:106518/1‑131 (MQ=255)                                                                                                   gcgGTTGAAGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAgc                                 <  1:106518/131‑1 (MQ=255)                                                                                                           aGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAgg                <  1:740658/140‑1 (MQ=255)                                                                                                           aGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAgg                <  1:532491/140‑1 (MQ=255)                                                                                                           aGTTCGTGAAGCGGTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAgg                <  1:255329/140‑1 (MQ=255)                                                                                                                        gTGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGCGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACaa   >  1:721431/1‑140 (MQ=255)                                                                                                                         tGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAAc  >  1:612568/1‑140 (MQ=255)                                                                                                                         tGCAGTTACTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAAc  >  1:48946/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                     TTGCCGAAGCGATTGTTGGCGTGGCTAACGGCGCTGGCGTGCGCACCACCGCGCTGCTCACCGACATGAATCAGGTACTGGCCTCCAGTGCAGGTAACGCGGTTGAAGTTCGTGAAGCGGTGCAGTTCCTGACGGGTGAATATCGTAACCCGCGTCTGTTTGATGTCACGATGGCGCTGTGCGTGGAGATGCTGATCTCCGGCAAACTGGCGAAAGATGACGCCGAAGCGCGCGCGAAATTGCAGGCGGTGCTGGACAAC  >  NC_000913/4618887‑4619146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑