Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	368,910	G→T	Q100H (CAG→CAT)	mhpA →	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	368,910	0	G	T	100.0%	102.8 / NA	30	Q100H (CAG→CAT)	mhpA	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/18);  total (12/18)

GCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAG  >  NC_000913/368776‑369044                                                                                                                                       |                                                                                                                                       gCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAAt                                                                                                                                   <  2:148597/140‑1 (MQ=255)      tGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCg                                                                                                                              <  1:657796/140‑1 (MQ=255)                     ggCGCTGCGCACCATGCAGTCGGTCGGCCTGTTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGGTGGCTCTCCCCGAAATGCCGCTGTTTTGCTGATAGTCATCCAATGACCGATCTAATttgctggc                                                                                                               >  2:750433/1‑140 (MQ=255)                     ggCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTtggctggc                                                                                                               >  2:603956/1‑140 (MQ=255)                     ggCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTtggctggc                                                                                                               >  2:462672/1‑140 (MQ=255)                       cgcTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCcg                                                                                                             <  1:462672/140‑1 (MQ=255)                          tGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCgcgc                                                                                                          >  2:162379/1‑140 (MQ=255)                               cccATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTaa                                                                                                     <  1:540433/139‑1 (MQ=255)                               aCCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTaa                                                                                                     <  1:238725/140‑1 (MQ=255)                                             cggCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGc                                                                                       >  2:628420/1‑140 (MQ=255)                                                    gTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGt                                                                                <  2:667043/140‑1 (MQ=255)                                                       gatgatGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGa                                                                             <  1:628420/140‑1 (MQ=255)                                                         tgatgTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATg                                                                           >  1:624941/1‑140 (MQ=255)                                                           atgTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCg                                                                         <  1:750433/140‑1 (MQ=255)                                                           atgTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCg                                                                         <  2:232213/140‑1 (MQ=255)                                                              ttCTGGCGTATACGACGACGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCAGCAGGTCGATGCgggg                                                                      >  2:305213/1‑138 (MQ=255)                                                              ttCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTg                                                                      <  1:357571/140‑1 (MQ=255)                                                              ttCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTg                                                                      <  1:162379/140‑1 (MQ=255)                                                                      cacaCTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGa                                                              >  2:759546/1‑140 (MQ=255)                                                                           tACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATg                                                                   >  2:258243/1‑130 (MQ=255)                                                                           tACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATg                                                                   <  1:258243/130‑1 (MQ=255)                                                                             cGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGgtg                                                       >  1:490574/1‑140 (MQ=255)                                                                             cGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGgtg                                                       >  1:393511/1‑140 (MQ=255)                                                                                  tGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTcgcg                                                  >  2:174511/1‑140 (MQ=255)                                                                                              cGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAAtgt                                      <  2:624941/140‑1 (MQ=255)                                                                                                 tttCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTgcg                                   <  2:490574/140‑1 (MQ=255)                                                                                                   tctcACCCCGAAAGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCgct                                 <  1:759546/140‑1 (MQ=255)                                                                                                             aaaGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCgctgc                               <  1:560548/132‑1 (MQ=255)                                                                                                             aaaGGCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCgctgc                               >  2:560548/1‑132 (MQ=255)                                                                                                                ggCCGCTGTTTTGCTGATATTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCcg                    <  1:174511/140‑1 (MQ=255)                                                                                                                                  atTCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTtcag  >  2:769425/1‑140 (MQ=255)                                                                                                                                    tCATCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTtc    <  2:521906/136‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GCGATTGGTATTGATGACGAGGCGCTGCGCACCATGCAGTCGGTCGGCCTGGTCGATGATGTTCTGCCGCACACTACGCCGTGGCACGCGATGCGTTTTCTCACCCCGAAAGGCCGCTGTTTTGCTGATATTCAGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAG  >  NC_000913/368776‑369044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑