Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,366,085	C→A	T179K (ACA→AAA)	puuE →	4‑aminobutyrate aminotransferase PuuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,366,085	0	C	A	100.0%	73.6 / NA	22	T179K (ACA→AAA)	puuE	4‑aminobutyrate aminotransferase PuuE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (15/7);  total (15/7)

TATATGACCATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTA  >  NC_000913/1365973‑1366219                                                                                                                 |                                                                                                                                       tataTGACCATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACg                                                                                                             <  1:73177/140‑1 (MQ=255)    atGACCATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCtt                                                                                                          >  1:100831/1‑140 (MQ=255)    atGACCATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCtt                                                                                                          >  2:607296/1‑140 (MQ=255)      gACCATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGt                                                                                                        <  1:327014/140‑1 (MQ=255)        ccATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGttt                                                                                                      >  2:213506/1‑140 (MQ=255)        ccATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGttt                                                                                                      >  1:91465/1‑140 (MQ=255)            ggCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAAt                                                                                                  >  1:100438/1‑140 (MQ=255)              cgcTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCa                                                                                                >  2:217068/1‑140 (MQ=255)                   aCCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACAt                                                                                           <  2:91465/140‑1 (MQ=255)                             ttGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATcgaagcgaag                                                                                 <  1:92364/140‑1 (MQ=255)                                                   cGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACCTCGAAGCGAAGCAGGGGGCGGCGATTATTTTCg                                                           <  2:100831/140‑1 (MQ=255)                                                       ccGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAAcc                                                       <  2:100438/140‑1 (MQ=255)                                                       ccGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAAcc                                                       <  1:607296/140‑1 (MQ=255)                                                                     ggTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCagggcgagg                                         >  1:755190/1‑140 (MQ=255)                                                                            cACGTACCTTATCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGttt                                  >  1:720799/1‑140 (MQ=255)                                                                                      aTCCGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTgcg                        >  2:3935/1‑140 (MQ=255)                                                                                         cGTCAGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCa                     >  2:275813/1‑140 (MQ=255)                                                                                             aGATTTACACGGCATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAag                 >  2:439011/1‑140 (MQ=255)                                                                                                         cATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCg     >  2:106705/1‑140 (MQ=255)                                                                                                         cATTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCg     >  1:428268/1‑140 (MQ=255)                                                                                                          aTTTCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGc    <  1:332104/140‑1 (MQ=255)                                                                                                            ttCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTa  >  2:282605/1‑140 (MQ=255)                                                                                                            ttCAAAACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTa  >  2:627359/1‑140 (MQ=255)                                                                                                                 |                                                                                                                                       TATATGACCATGGCGCTGACCGGAAAAGTTGCGCCGTACAAAATCGGCTTCGGCCCGTTCCCTGGTTCGGTGTATCACGTACCTTATCCGTCAGATTTACACGGCATTTCAACACAGGACTCCCTCGACGCCATCGAACGCTTGTTTAAATCAGACATCGAAGCGAAGCAGGTGGCGGCGATTATTTTCGAACCGGTGCAGGGCGAGGGCGGTTTCAACGTTGCGCCAAAAGAGCTGGTTGCCGCTA  >  NC_000913/1365973‑1366219

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑