Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,608,061	C→A	S537R (AGC→AGA)	zntA →	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,608,061	0	C	A	100.0%	236.7 / NA	67	S537R (AGC→AGA)	zntA	Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (30/37);  total (30/37)

CCGCCGAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGCGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTGAT  >  NC_000913/3607931‑3608192                                                                                                                                   |                                                                                                                                    ccgccgAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCaa                                                                                                                            >  2:98595/1‑140 (MQ=255)    ccgAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACg                                                                                                                         <  2:157265/140‑1 (MQ=255)      gAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGt                                                                                                                       <  1:1269020/140‑1 (MQ=255)       aaTCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTa                                                                                                                      <  2:865705/140‑1 (MQ=255)          cacaGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTg                                                                                                                   <  1:1115013/140‑1 (MQ=255)          cacaGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTg                                                                                                                   <  1:98595/140‑1 (MQ=255)          cacaGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTg                                                                                                                   <  1:490205/140‑1 (MQ=255)           acacCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGc                                                                                                                  <  1:632988/140‑1 (MQ=255)           acaGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGc                                                                                                                  <  1:1312453/140‑1 (MQ=255)             aGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTg                                                                                                                <  2:1336244/140‑1 (MQ=255)               cGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGgt                                                                                                              <  2:851405/140‑1 (MQ=255)                gggCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGgta                                                                                                             <  1:12990/140‑1 (MQ=255)                  gcgcTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGgtagt                                                                                                           >  1:654939/1‑140 (MQ=255)                    gcTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTAc                                                                                                         >  1:848659/1‑140 (MQ=255)                     cTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACg                                                                                                        <  1:955078/140‑1 (MQ=255)                          cGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACg                                                                                                   <  2:654939/140‑1 (MQ=255)                              tCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGa                                                                                               <  2:572985/140‑1 (MQ=255)                                tGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACg                                                                                             <  1:306878/140‑1 (MQ=255)                                tGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACg                                                                                             <  2:766602/140‑1 (MQ=255)                                  gCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTg                                                                                           >  2:389879/1‑140 (MQ=255)                                        aaGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGgt                                                                                     >  1:324609/1‑140 (MQ=255)                                          gcgcAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGgtgt                                                                                   >  1:1361551/1‑140 (MQ=255)                                             cAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCAt                                                                                <  2:848659/140‑1 (MQ=255)                                             cAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCAt                                                                                >  2:1046508/1‑140 (MQ=255)                                               ggTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTg                                                                              <  2:399836/140‑1 (MQ=255)                                               ggTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTg                                                                              <  1:389879/140‑1 (MQ=255)                                               ggTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTg                                                                              <  2:324609/140‑1 (MQ=255)                                                   aaCGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGtt                                                                          <  1:730358/140‑1 (MQ=255)                                                   aaCGGTGAGCGCGTATCGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGtt                                                                          <  2:111327/140‑1 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCa                                                                                 <  1:249926/126‑1 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCa                                                                                 >  2:249926/1‑126 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCAt                                                                                >  1:1360644/1‑127 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCAt                                                                                <  2:1360644/127‑1 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCg                                                                            >  2:450197/1‑131 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCg                                                                            <  1:450197/131‑1 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGt                                                                           >  1:447450/1‑132 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGt                                                                           <  2:447450/132‑1 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGAt                                                                   >  1:838649/1‑140 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGAt                                                                   >  1:726813/1‑140 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGAt                                                                   >  1:337484/1‑140 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGAt                                                                   >  1:1162001/1‑140 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGCGTCATTg                                                                              >  2:1225640/1‑129 (MQ=255)                                                          aGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGCGTCATTg                                                                              <  1:1225640/129‑1 (MQ=255)                                                                          cgcTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGAtg                                                   >  2:1254981/1‑140 (MQ=255)                                                                               ccGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCa                                              >  1:340289/1‑140 (MQ=255)                                                                                     aaCATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGcc                                        <  1:1046508/140‑1 (MQ=255)                                                                                         tCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGc                                         <  1:1063711/135‑1 (MQ=255)                                                                                         tCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGc                                         >  2:1063711/1‑135 (MQ=255)                                                                                                       ttACTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAAcgcg                      <  1:1254981/140‑1 (MQ=255)                                                                                                         aCTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCt                    <  2:340289/140‑1 (MQ=255)                                                                                                         aCTGGTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCt                    >  1:382931/1‑140 (MQ=255)                                                                                                            ggTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTggg                 <  1:1337228/140‑1 (MQ=255)                                                                                                             gTTTAATTAACGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGNNNTGGGc                >  2:222192/1‑140 (MQ=255)                                                                                                                taattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTc             >  2:338691/1‑140 (MQ=255)                                                                                                                taattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTc             >  2:705233/1‑140 (MQ=255)                                                                                                                 aattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCa            >  2:1138433/1‑140 (MQ=255)                                                                                                                 aattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCa            <  2:382931/140‑1 (MQ=255)                                                                                                                 aattaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCa            <  1:222192/140‑1 (MQ=255)                                                                                                                   ttaaCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCaaa          >  2:40248/1‑140 (MQ=255)                                                                                                                      aCGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAggg       <  1:1138433/140‑1 (MQ=255)                                                                                                                       cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGt              <  2:843137/132‑1 (MQ=255)                                                                                                                       cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGt              >  1:843137/1‑132 (MQ=255)                                                                                                                       cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAgggg      >  1:23929/1‑140 (MQ=255)                                                                                                                       cGAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTAGGCGTCAAAgggg      >  2:1095078/1‑140 (MQ=255)                                                                                                                        gAACTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGt     >  1:55556/1‑140 (MQ=255)                                                                                                                        gAACTGGAAAGAGCCGGGCAAACGGTAGTGCCGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGt     >  1:1302987/1‑140 (MQ=255)                                                                                                                           cTGGAAAGAGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTGAt  <  1:705233/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                    CCGCCGAATCACAGCGGGCGCTGGTCGGGTCTGGCATTGAAGCGCAGGTTAACGGTGAGCGCGTATTGATTTGCGCTGCCGGGAAACATCCCGCTGATGCATTTACTGGTTTAATTAACGAACTGGAAAGCGCCGGGCAAACGGTAGTGCTGGTAGTACGTAACGATGACGTGCTTGGTGTCATTGCGTTACAGGATACCCTGCGCGCCGATGCTGCAACTGCCATCAGTGAACTGAACGCGCTGGGCGTCAAAGGGGTGAT  >  NC_000913/3607931‑3608192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑