BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,261,710	C→A	S14R (AGC→AGA)	dusA →	tRNA‑dihydrouridine synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,261,710	0	C	A	100.0%	495.8 / NA	139	S14R (AGC→AGA)	dusA	tRNA‑dihydrouridine synthase A
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (74/65); total (74/65)

Only 100 of 139 total aligned reads displayed.

GTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261579‑4261844

gTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGcc                                                                                                                                >  1:841049/1‑140 (MQ=255)

 tataGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCt                                                                                                                               <  1:719727/140‑1 (MQ=255)

  ataGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTg                                                                                                                              >  2:941423/1‑140 (MQ=255)

     gTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaa                                                                                                                           >  1:1079201/1‑140 (MQ=255)

     gTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaa                                                                                                                           >  2:1132998/1‑140 (MQ=255)

        ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                        >  1:174101/1‑140 (MQ=255)

        ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                        >  1:1020917/1‑140 (MQ=255)

        ccccGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATACAAAAAATCAACCAAACCAGAGCAATGCCTGaaaaaa                                                                                                                        >  1:367709/1‑140 (MQ=255)

           cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  1:1037671/1‑140 (MQ=255)

           cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  1:897607/1‑140 (MQ=255)

           cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     <  2:1037671/140‑1 (MQ=255)

           cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  2:92104/1‑140 (MQ=255)

           cGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTg                                                                                                                     >  2:1230984/1‑140 (MQ=255)

             cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   >  1:120101/1‑140 (MQ=255)

             cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   >  1:642577/1‑140 (MQ=255)

             cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   >  1:409997/1‑140 (MQ=255)

             cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   <  2:538613/140‑1 (MQ=255)

             cTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGAc                                                                                                                   >  2:452181/1‑140 (MQ=255)

              tATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACg                                                                                                                  <  2:409997/140‑1 (MQ=255)

                  gAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCa                                                                                                              <  1:408959/140‑1 (MQ=255)

                     cctcctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTg                                                                                                           >  1:877840/1‑140 (MQ=255)

                        cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAg                                                                                                        >  2:172717/1‑140 (MQ=255)

                        cctGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAg                                                                                                        >  2:1096167/1‑140 (MQ=255)

                          tGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTg                                                                                                      >  2:291402/1‑140 (MQ=255)

                          tGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTg                                                                                                      <  1:377224/140‑1 (MQ=255)

                            aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  2:652158/140‑1 (MQ=255)

                            aaTAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  2:609572/140‑1 (MQ=255)

                                gTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGt                                                                                                >  2:1340409/1‑140 (MQ=255)

                                   aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    >  2:135553/1‑133 (MQ=255)

                                   aTGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGt                                                                                                    <  1:135553/133‑1 (MQ=255)

                                    tGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAg                                                                                            <  2:1390384/140‑1 (MQ=255)

                                      cTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCg                                                                                          >  2:643448/1‑140 (MQ=255)

                                       tGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGt                                                                                         <  2:49142/140‑1 (MQ=255)

                                        gAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGtt                                                                                        <  1:172717/140‑1 (MQ=255)

                                              cataacCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCa                                                                                  >  1:590445/1‑140 (MQ=255)

                                                   ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:966424/1‑140 (MQ=255)

                                                   ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:625819/1‑140 (MQ=255)

                                                   ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:884442/1‑140 (MQ=255)

                                                   ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  2:271951/1‑140 (MQ=255)

                                                   ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:754467/1‑140 (MQ=255)

                                                   ccccATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCt                                                                             >  1:10385/1‑140 (MQ=255)

                                                      cATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGa                                                                          <  1:196923/140‑1 (MQ=255)

                                                        tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  1:1276696/1‑140 (MQ=255)

                                                        tGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACt                                                                        >  2:812446/1‑140 (MQ=255)

                                                         gATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTg                                                                       <  2:1437190/140‑1 (MQ=255)

                                                         gATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTg                                                                       >  1:507077/1‑140 (MQ=255)

                                                          atatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgg                                                                      <  2:564370/140‑1 (MQ=255)

                                                           tatatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAGATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTgga                                                                     <  2:322676/140‑1 (MQ=255)

                                                             tatCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacg                                                                   >  1:1280353/1‑140 (MQ=255)

                                                              atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:1367124/1‑140 (MQ=255)

                                                              atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  1:566316/1‑140 (MQ=255)

                                                              atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  <  1:941423/140‑1 (MQ=255)

                                                              atCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacgg                                                                  >  2:1457536/1‑140 (MQ=255)

                                                                cGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacggac                                                                >  1:717732/1‑140 (MQ=255)

                                                                cGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTggacggac                                                                >  1:247031/1‑140 (MQ=255)

                                                                 gATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                               <  2:10385/140‑1 (MQ=255)

                                                                  aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  2:531280/1‑140 (MQ=255)

                                                                  aTAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacag                                                              >  1:1195078/1‑140 (MQ=255)

                                                                   tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                             >  2:759576/1‑140 (MQ=255)

                                                                   tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                             >  1:148744/1‑140 (MQ=255)

                                                                   tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                             <  2:754467/140‑1 (MQ=255)

                                                                   tAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgacaga                                                             >  2:765288/1‑140 (MQ=255)

                                                                      aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGAc                                                                         <  1:924439/125‑1 (MQ=255)

                                                                      aataatCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          <  2:507077/140‑1 (MQ=255)

                                                                          atCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGcc                                                      <  1:1230984/140‑1 (MQ=255)

                                                                           tctcTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCg                                                     <  1:1132998/140‑1 (MQ=255)

                                                                             tctACATTTGAAAATGCACGGTAATTCTGAAGTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   <  1:756922/140‑1 (MQ=255)

                                                                             tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACAt                                                          <  2:895889/133‑1 (MQ=255)

                                                                             tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   <  2:86028/140‑1 (MQ=255)

                                                                             tctACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCt                                                   >  2:90247/1‑140 (MQ=255)

                                                                               tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                               >  2:1414198/1‑126 (MQ=255)

                                                                               tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGgaca                                                               <  1:1414198/126‑1 (MQ=255)

                                                                               tACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTAt                                                 >  2:1178272/1‑140 (MQ=255)

                                                                                   tttGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCt                                             <  1:90247/140‑1 (MQ=255)

                                                                                           tGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTg                                     >  1:7567/1‑140 (MQ=255)

                                                                                              aCGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCtt                                  <  1:1096167/140‑1 (MQ=255)

                                                                                                ggTAATTCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTc                                <  2:236270/140‑1 (MQ=255)

                                                                                                     ttCTGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           >  1:1415943/1‑140 (MQ=255)

                                                                                                        tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  1:1457536/140‑1 (MQ=255)

                                                                                                        tGAAATGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATa                        <  2:632814/140‑1 (MQ=255)

                                                                                                          aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           >  2:311877/1‑135 (MQ=255)

                                                                                                          aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCa                           <  1:311877/135‑1 (MQ=255)

                                                                                                          aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:1079201/140‑1 (MQ=255)

                                                                                                          aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:160049/140‑1 (MQ=255)

                                                                                                          aaaTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACg                      <  2:1226475/140‑1 (MQ=255)

                                                                                                            aTGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGtt                    >  1:1404310/1‑140 (MQ=255)

                                                                                                             tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:661810/140‑1 (MQ=255)

                                                                                                             tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  1:643448/140‑1 (MQ=255)

                                                                                                             tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:1223845/140‑1 (MQ=255)

                                                                                                             tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:7567/140‑1 (MQ=255)

                                                                                                             tGCAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:590445/140‑1 (MQ=255)

                                                                                                               cAAAAAATCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACAGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCt                 >  2:1055829/1‑140 (MQ=255)

                                                                                                                  aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  1:340363/140‑1 (MQ=255)

                                                                                                                  aaaaTCAACCAAACCAGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGta              <  2:1404310/140‑1 (MQ=255)

                                                                                                                         accaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   >  1:295670/1‑128 (MQ=255)

                                                                                                                         accaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTg                   <  2:295670/128‑1 (MQ=255)

                                                                                                                         accaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaa       <  2:877840/140‑1 (MQ=255)

                                                                                                                         accaaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGaa       <  1:966424/140‑1 (MQ=255)

                                                                                                                            aaaccaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATg    <  2:36720/140‑1 (MQ=255)

                                                                                                                              accaGAGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGt  >  2:396662/1‑140 (MQ=255)

GTATAGTACCCCGCTATTGAGCCTCCTGAATAGTGATGCTGAATAACATAACCCCATGATATATCGATAAAATAATCTCTACATTTGAAAATGCACGGTAATTCTGAAATGCAAAAAATCAACCAAACCAGCGCAATGCCTGAAAAAACTGACGTTCACTGGAGTGGTCGGTTTAGCGTTGCACCAATGCTCGACTGGACGGACAGACATTGCCGCTATTTCTTGCGTCTGCTTTCCCGCAATACGTTGCTGTATACCGAAATGGT  >  NC_000913/4261579‑4261844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`