BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,655,181	T→C	intergenic (‑40/+166)	rspA ← / ← ynfA	mandelate racemase/muconate lactonizing enzyme family protein RspA/conserved inner membrane protein YnfA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,655,181	0	T	C	100.0%	391.9 / NA	102	intergenic (‑40/+166)	rspA/ynfA	mandelate racemase/muconate lactonizing enzyme family protein RspA/conserved inner membrane protein YnfA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (44/58); total (44/58)

Only 100 of 103 total aligned reads displayed.

CAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTC  >  NC_000913/1655048‑1655316

cAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCaa                                                                                                                                   >  1:89336/1‑140 (MQ=255)

 aaGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAAc                                                                                                                                  <  2:967139/140‑1 (MQ=255)

 aaGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAAc                                                                                                                                  <  2:1377231/140‑1 (MQ=255)

 aaGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAAc                                                                                                                                  <  1:876557/140‑1 (MQ=255)

    gCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGa                                                                                                                               <  1:1064034/140‑1 (MQ=255)

      ccGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGc                                                                                                                             <  2:516134/140‑1 (MQ=255)

      ccGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGc                                                                                                                             <  2:461657/140‑1 (MQ=255)

       cGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGct                                                                                                                            >  1:1167400/1‑140 (MQ=255)

       cGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGct                                                                                                                            >  1:829183/1‑140 (MQ=255)

        gTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGcta                                                                                                                           <  1:865959/140‑1 (MQ=255)

        gTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGcta                                                                                                                           <  1:1291018/140‑1 (MQ=255)

         tAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAAc                                                                                                                                  >  1:213188/1‑132 (MQ=255)

         tAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAAc                                                                                                                                  <  2:213188/132‑1 (MQ=255)

         tAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctac                                                                                                                          <  1:435274/140‑1 (MQ=255)

         tAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctac                                                                                                                          >  1:823411/1‑140 (MQ=255)

         tAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctac                                                                                                                          <  2:173411/140‑1 (MQ=255)

         tAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGc                                                                                                                             <  1:1390086/137‑1 (MQ=255)

         tAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGc                                                                                                                             >  2:1390086/1‑137 (MQ=255)

           aTACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctacta                                                                                                                        <  1:1447270/140‑1 (MQ=255)

           aTACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctacta                                                                                                                        <  2:925625/140‑1 (MQ=255)

              ccGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctac                                                                                                                          >  2:573831/1‑135 (MQ=255)

              ccGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCa                                                                                                                     <  1:1215911/140‑1 (MQ=255)

                gTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACt                                                                                                                                 <  1:868595/126‑1 (MQ=255)

                gTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACt                                                                                                                                 >  2:868595/1‑126 (MQ=255)

                gTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATa                                                                                                                   >  1:919608/1‑140 (MQ=255)

                gTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATa                                                                                                                   <  1:722161/140‑1 (MQ=255)

                 tCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATAc                                                                                                                  >  2:164855/1‑140 (MQ=255)

                 tCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATAc                                                                                                                  >  1:298456/1‑140 (MQ=255)

                  ccTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACa                                                                                                                 >  1:1057614/1‑140 (MQ=255)

                   cTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGc                                                                                                                             >  2:758896/1‑127 (MQ=255)

                   cTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGc                                                                                                                             <  1:758896/127‑1 (MQ=255)

                   cTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCa                                                                                                                     <  1:1127369/135‑1 (MQ=255)

                   cTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCa                                                                                                                     >  2:1127369/1‑135 (MQ=255)

                   cTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACaa                                                                                                                >  1:604127/1‑140 (MQ=255)

                      aGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGta                                                                                                             >  1:66695/1‑140 (MQ=255)

                        tggtgATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctac                                                                                                                          <  2:648276/125‑1 (MQ=255)

                        tggtgATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGctac                                                                                                                          >  1:648276/1‑125 (MQ=255)

                             aTTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGAt                                                                                                      <  2:505842/140‑1 (MQ=255)

                                    aTGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGaaaaa                                                                                               >  1:1082087/1‑140 (MQ=255)

                                    aTGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGaaaaa                                                                                               >  2:1137140/1‑140 (MQ=255)

                                         aCGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGaaa                                                                                                 <  1:213085/133‑1 (MQ=255)

                                         aCGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGaaa                                                                                                 >  2:213085/1‑133 (MQ=255)

                                         aCGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCg                                                                                          >  2:122395/1‑140 (MQ=255)

                                           gAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGa                                                                                        <  1:527683/140‑1 (MQ=255)

                                           gAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTATACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGa                                                                                        <  1:514115/140‑1 (MQ=255)

                                                 aCGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATc                                                                                  <  2:1057614/140‑1 (MQ=255)

                                                         gACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACaaaaaa                                                                          >  2:376389/1‑140 (MQ=255)

                                                           cAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAg                                                                        <  2:89336/140‑1 (MQ=255)

                                                              gTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCg                                                                     <  2:829183/140‑1 (MQ=255)

                                                                 aCAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGtat                                                                  >  1:285059/1‑140 (MQ=255)

                                                                 aCAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGta                                                                   <  2:386245/139‑1 (MQ=255)

                                                                 aCAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGta                                                                   >  1:386245/1‑139 (MQ=255)

                                                                    aaaaCTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATAtt                                                               <  1:418062/140‑1 (MQ=255)

                                                                      aaCTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTg                                                             <  2:823411/140‑1 (MQ=255)

                                                                        cTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATAttt                                                              >  2:366148/1‑137 (MQ=255)

                                                                        cTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATAttt                                                              <  1:366148/137‑1 (MQ=255)

                                                                        cTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTgcg                                                           <  1:360363/140‑1 (MQ=255)

                                                                        cTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTgcg                                                           <  1:131606/140‑1 (MQ=255)

                                                                           cAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCtg                                                        >  1:664731/1‑140 (MQ=255)

                                                                               cTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGaa                                                    <  1:376389/140‑1 (MQ=255)

                                                                                 ttACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATg                                                  >  2:641180/1‑140 (MQ=255)

                                                                                    cGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGtt                                               <  1:1185176/140‑1 (MQ=255)

                                                                                        cTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACa                                           <  2:1167400/140‑1 (MQ=255)

                                                                                        cTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACa                                           <  1:1308608/140‑1 (MQ=255)

                                                                                          tCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACaaa                                         <  1:450885/140‑1 (MQ=255)

                                                                                           cATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACaaaa                                        >  2:102992/1‑140 (MQ=255)

                                                                                             tGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGa                                      >  2:132605/1‑140 (MQ=255)

                                                                                             tGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGa                                      <  2:224967/140‑1 (MQ=255)

                                                                                               ttCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATg                                    <  2:690396/140‑1 (MQ=255)

                                                                                                tCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGa                                   <  1:1355990/140‑1 (MQ=255)

                                                                                                 cGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGaa                                                    <  2:882831/122‑1 (MQ=255)

                                                                                                 cGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGaa                                                    >  1:882831/1‑122 (MQ=255)

                                                                                                 cGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGaa                                  >  1:1131349/1‑140 (MQ=255)

                                                                                                 cGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGaa                                  >  1:1264777/1‑140 (MQ=255)

                                                                                                      ccTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGa                             >  1:679322/1‑140 (MQ=255)

                                                                                                       cTTGCATCGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGaa                            <  1:1352501/140‑1 (MQ=255)

                                                                                                              cGCTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCttt                     >  1:932197/1‑140 (MQ=255)

                                                                                                                cTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                   >  2:168631/1‑140 (MQ=255)

                                                                                                                cTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                   >  2:92754/1‑140 (MQ=255)

                                                                                                                cTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                   >  1:1406446/1‑140 (MQ=255)

                                                                                                                cTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                   >  2:159130/1‑140 (MQ=255)

                                                                                                                cTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                   >  2:662195/1‑140 (MQ=255)

                                                                                                                cTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                   >  1:851753/1‑140 (MQ=255)

                                                                                                                cTTGTCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTg                   <  2:132254/140‑1 (MQ=255)

                                                                                                                    tCGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAg               >  1:162819/1‑140 (MQ=255)

                                                                                                                     cGTGATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGc              >  2:278312/1‑140 (MQ=255)

                                                                                                                        gATGCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGa           <  2:1269548/140‑1 (MQ=255)

                                                                                                                           gCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGacac        <  2:1264777/140‑1 (MQ=255)

                                                                                                                           gCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGacac        <  2:679322/140‑1 (MQ=255)

                                                                                                                           gCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGacac        >  1:809128/1‑140 (MQ=255)

                                                                                                                           gCATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGacac        <  2:919608/140‑1 (MQ=255)

                                                                                                                            cATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACt       <  1:168631/140‑1 (MQ=255)

                                                                                                                            cATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACt       <  1:164855/140‑1 (MQ=255)

                                                                                                                            cATGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACt       <  1:662195/140‑1 (MQ=255)

                                                                                                                             aTGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACtt      <  2:66695/140‑1 (MQ=255)

                                                                                                                             aTGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACtt      <  1:132605/140‑1 (MQ=255)

                                                                                                                              tGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTc     <  2:809128/140‑1 (MQ=255)

                                                                                                                              tGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTc     >  2:134920/1‑140 (MQ=255)

                                                                                                                              tGAAATCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTc     >  2:558519/1‑140 (MQ=255)

                                                                                                                                 aaTCCACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCctc  <  2:664731/140‑1 (MQ=255)

CAAGGCCCGTAATACCGTCCTCAGTGGTGATTTTTAATGTGACGAAATTACGCCCCGGACAGGTAACAAAAACTTCAGCCTTTACGATCTTCATGTTCGATTCCTTGCATCGCTTGTCGTGATGCATGAAATCTACGCAACTGAGCTACTACCATACAAGTATAAAGATCGAAAAAAGCCGGAGTGATCACAAAAAAAGGCGTATATTTGCGCTGTGAATGGTTGACAAAAGATGAAATAGAATACCTTTTGTCAGCTGACACTTCCTC  >  NC_000913/1655048‑1655316

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`