Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,791,005	C→A	A80A (GCG→GCT)	ydiU ←	UPF0061 family protein YdiU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,791,005	0	C	A	100.0%	76.2 / NA	23	A80A (GCG→GCT)	ydiU	UPF0061 family protein YdiU
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (11/12);  total (11/12)

AACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGC  >  NC_000913/1790876‑1791133                                                                                                                                  |                                                                                                                                 aaCCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGc                                                                                                                        >  1:222974/1‑140 (MQ=255)       ccGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCg                                                                                                                      <  1:447956/136‑1 (MQ=255)       ccGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCg                                                                                                                      >  2:447956/1‑136 (MQ=255)                    aTTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTa                                                                                                     >  1:69476/1‑140 (MQ=255)                     ttCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTaa                                                                                                    >  2:200162/1‑140 (MQ=255)                     ttCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTaa                                                                                                    >  2:352956/1‑140 (MQ=255)                             tAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTgcgc                                                                                            <  1:228177/140‑1 (MQ=255)                                ggCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAg                                                                                         <  1:10059/140‑1 (MQ=255)                                      aGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGa                                                                                   <  1:115942/140‑1 (MQ=255)                                             cACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCa                                                                            <  1:200162/140‑1 (MQ=255)                                              aCCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAg                                                                           >  2:216561/1‑140 (MQ=255)                                                  ttCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAg                                                                       >  2:107323/1‑140 (MQ=255)                                                             gTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcgc                                                            <  2:288037/140‑1 (MQ=255)                                                              tCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTcgcc                                                           >  2:377143/1‑140 (MQ=255)                                                                    gTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCa                                                     >  2:360183/1‑140 (MQ=255)                                                                           cATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCACAGACGCCGAACTGATGGCCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCt                                              <  2:325442/140‑1 (MQ=255)                                                                            aTCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTg                                             >  1:414598/1‑140 (MQ=255)                                                                               agcaagcaGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTgcgc                                          <  2:109149/140‑1 (MQ=255)                                                                                    gcaGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCAttt                                     <  2:57070/140‑1 (MQ=255)                                                                                               ccGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGcga                          <  1:77244/140‑1 (MQ=255)                                                                                                 gAGTAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGcgacg                        <  2:114431/140‑1 (MQ=255)                                                                                                    tAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATg                     <  1:100159/140‑1 (MQ=255)                                                                                                    tAAAATGCCGCGCCCATCACCCAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATg                     >  1:418657/1‑140 (MQ=255)                                                                                                                       cccAGTTGGCCAGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGc  <  2:64569/140‑1 (MQ=255)                                                                                                                                  |                                                                                                                                 AACCGCCCGTCCATCACCCATTCGCGAATAAGGCGTCAGGCCAGCACCTTTCAGATGCCAGTCCATTGTAGTGCCATCAGCAAGCAGTTGTTCGCCGAGTAAAATGCCGCGCCCATCACCCAGTTGGCCCGCCCAGACGCCGAACTGATGACCACTGTAAACCTGCGCCAGTGGTGACATACCAGGCAGTAGGGCTTCGCCGCCCCAGACACCTGCGCCATTTTTAAACAGCGACGATGGAATACTCAGCGTGTTAGC  >  NC_000913/1790876‑1791133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑