Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,830,164	G→T	A201E (GCA→GAA)	astA ←	arginine N‑succinyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,830,164	0	G	T	100.0%	73.4 / NA	22	A201E (GCA→GAA)	astA	arginine N‑succinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/8);  total (14/8)

CGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATT  >  NC_000913/1830032‑1830300                                                                                                                                     |                                                                                                                                         cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTg                                                                                                                                   >  1:395251/1‑140 (MQ=255) cGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTg                                                                                                                                   >  2:245910/1‑140 (MQ=255)   aaaaCCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGcc                                                                                                                                 <  2:160020/140‑1 (MQ=255)                        cgcgcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCgg                                                                                                            >  1:324778/1‑140 (MQ=255)                        cgcgcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCgg                                                                                                            >  1:38830/1‑140 (MQ=255)                        cgcgcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCgg                                                                                                            >  2:284267/1‑140 (MQ=255)                          cgcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg                                                                                                          >  2:197301/1‑140 (MQ=255)                          cgcgGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGgcg                                                                                                          >  2:140340/1‑140 (MQ=255)                                         tttGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATc                                                                                           <  2:395251/140‑1 (MQ=255)                                            gCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGaa                                                                                        <  2:141175/140‑1 (MQ=255)                                                                   tCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTg                                                                 >  2:65299/1‑140 (MQ=255)                                                                    ccTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGc                                                                >  1:361797/1‑140 (MQ=255)                                                                                gggATAAAAAGTGGGTATAGATCGGATGTTTAGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAACTCCATCGAAACGAAGCGTTTACCGAGACTTTGCCAGAACGGTGaa                                                    <  2:392787/140‑1 (MQ=255)                                                                                                   gATCGGATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCa                                 >  1:9560/1‑140 (MQ=255)                                                                                                        gATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCcg                            >  2:97283/1‑140 (MQ=255)                                                                                                        gATGTTTCGGCATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCcg                            >  1:71342/1‑140 (MQ=255)                                                                                                                  cATCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAg                  <  1:97283/140‑1 (MQ=255)                                                                                                                   aTCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGc                 <  1:419574/140‑1 (MQ=255)                                                                                                                    tCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCa                <  1:50258/140‑1 (MQ=255)                                                                                                                    tCAGTTCTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCa                <  1:214448/140‑1 (MQ=255)                                                                                                                          cTGCAATAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACt          <  1:197301/140‑1 (MQ=255)                                                                                                                                tAAATTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCa    >  1:57603/1‑140 (MQ=255)                                                                                                                                  aaTTCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCAtt  >  1:143259/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                         CGAAAACCTTCTTTCTCCAGCACCGCGCGGGCAGGCGCGGTTTGCGGATGTACCTGACCGATGACGTCCTGGGCTTCCTGGGATAAAAAGTGGGTATAGATCGGATGTTTCGGCATCAGTTCTGCAATAAATGCCTTTTGCCCGGTGCCGCAGAGAAAATCGGCGCGGCTAAAATCCATCGAAAAGAAGCGTTTACCGAGGCTTTGCCAGAACGGTGAATAGCCGTGTTCGTCAATCACCCCGCGCATTTCAGCAACCACTTTGTCATT  >  NC_000913/1830032‑1830300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑