Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,352,538	G→T	intergenic (‑164/‑109)	glpT ← / → glpA	sn‑glycerol 3‑phosphate:phosphate antiporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,352,538	0	G	T	100.0%	91.3 / NA	27	intergenic (‑164/‑109)	glpT/glpA	sn‑glycerol 3‑phosphate:phosphate antiporter/anaerobic glycerol‑3‑phosphate dehydrogenase subunit A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/11);  total (16/11)

GATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTG  >  NC_000913/2352407‑2352674                                                                                                                                    |                                                                                                                                         gATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAAt                                                                                                                                  >  2:24617/1‑140 (MQ=255) gATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAAt                                                                                                                                  >  2:239113/1‑140 (MQ=255) gATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAAt                                                                                                                                  >  2:430292/1‑140 (MQ=255)                    tGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAAtt                                                                                                               >  2:355510/1‑140 (MQ=255)                         gaACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAg                                                                                                          >  2:265164/1‑140 (MQ=255)                                   tttCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATgcg                                                                                                <  2:347453/140‑1 (MQ=255)                                             tGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCaaa                                                                                      <  2:321503/140‑1 (MQ=255)                                             tGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCaaa                                                                                      >  1:343476/1‑140 (MQ=255)                                               ccGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACa                                                                                    >  1:56561/1‑140 (MQ=255)                                                  cgATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAAtt                                                                                 <  2:333573/140‑1 (MQ=255)                                                    aTGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCa                                                                               <  1:111208/140‑1 (MQ=255)                                                              aaCATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACt                                                                     <  1:130570/140‑1 (MQ=255)                                                                 aTTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATg                                                                  >  2:293197/1‑140 (MQ=255)                                                                 aTTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATg                                                                  >  1:220033/1‑140 (MQ=255)                                                                   tCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGc                                                                >  1:7080/1‑140 (MQ=255)                                                                      taaattaaatGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATAACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTaa                                                             >  1:88318/1‑140 (MQ=255)                                                                                  gAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAAc                                                 <  1:24617/140‑1 (MQ=255)                                                                                   aaTTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACg                                                <  1:38318/140‑1 (MQ=255)                                                                                         cGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTc                                          <  1:239113/140‑1 (MQ=255)                                                                                            acacaTTATTAAATAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCaga                                       <  1:265164/140‑1 (MQ=255)                                                                                                         tAAGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGa                          >  2:64600/1‑140 (MQ=255)                                                                                                          aaGATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGaa                         >  2:105125/1‑140 (MQ=255)                                                                                                            gATTTACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGaaaa                       >  2:321406/1‑140 (MQ=255)                                                                                                                tACAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTcg                   <  1:355510/140‑1 (MQ=255)                                                                                                                 aCAAAATGTTCAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTcgc                  >  2:216038/1‑140 (MQ=255)                                                                                                                           cAAAATGACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAAt        >  1:339478/1‑140 (MQ=255)                                                                                                                                 gACTCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTg  <  2:84317/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                         GATTAACAGCCTGATTCAGTGAGAGAACCTGCCGTTTCTTGAGTTGCCGCGATGTTAAGAAAACATTCATAAATTAAATGTGAATTGCCGCACACATTATTAAATAAGATTTACAAAATGTTCAAAATGACGCATGAAATCACGTTTCACTTTCGAATTATGAGCGAATATGCGCGAAATCAAACAATTCATGTTTTTACTATGGCTAAATGGTAAAAAACGAACTTCAGAGGGATAACAATGAAAACTCGCGACTCGCAATCAAGTG  >  NC_000913/2352407‑2352674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑