Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,797,105	C→A	intergenic (‑77/‑106)	yqaE ← / → ygaV	Pmp3 family protein/putative DNA‑binding transcriptional regulator YgaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,797,105	0	C	A	100.0%	66.1 / NA	20	intergenic (‑77/‑106)	yqaE/ygaV	Pmp3 family protein/putative DNA‑binding transcriptional regulator YgaV
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/8);  total (12/8)

GCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCCTGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTTAAATAAATATGACTGAACTCGCGCA  >  NC_000913/2796974‑2797227                                                                                                                                    |                                                                                                                           gcagcaCGCCGAGCGGCGGCAGAATGATGGTGAAGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGtt                                                                                                                    >  1:20455/1‑140 (MQ=255)     caCGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAg                                                                                                                >  1:388381/1‑140 (MQ=255)                   gcAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTAct                                                                                                  <  2:321062/140‑1 (MQ=255)                                  tgaCGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACgctgct                                                                                   <  1:219239/140‑1 (MQ=255)                                     cGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAg                                                                                <  2:154885/140‑1 (MQ=255)                                           tcCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGt                                                                          <  1:28333/140‑1 (MQ=255)                                                              tCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTtttagtt                                                       >  1:111523/1‑140 (MQ=255)                                                               cccTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTtttagttt                                                      >  2:360556/1‑140 (MQ=255)                                                                  tataAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTaga                                                   >  2:403491/1‑140 (MQ=255)                                                                   ataAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTagag                                                  >  1:222153/1‑140 (MQ=255)                                                                     aaGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGtt                                                >  2:382163/1‑140 (MQ=255)                                                                         aaaTTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACt                                            <  2:61047/140‑1 (MQ=255)                                                                            ttACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTaa                                         >  2:166059/1‑140 (MQ=255)                                                                                    tGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGaaa                                 <  2:284987/140‑1 (MQ=255)                                                                                     gTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGt                                                 <  2:817/123‑1 (MQ=255)                                                                                     gTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGt                                                 >  1:817/1‑123 (MQ=255)                                                                                                gaTTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGTATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTtaaataa                     >  1:324036/1‑140 (MQ=255)                                                                                                            tAAGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTTAAATAAATATGACTGAAc         >  2:37325/1‑140 (MQ=255)                                                                                                             aaGCGTAGTAATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTTAAATAAATATGACTGAACt        <  1:253538/140‑1 (MQ=255)                                                                                                                   agtaATTTTAGCGGAGCATGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTTAAATAAATATGACTGAACTCGCGCa  >  1:122579/1‑140 (MQ=255)                                                                                                                                    |                                                                                                                           GCAGCACGCCGAGCGGCGGCAGAATGATGGTGATGACGATTCTCCAGAAACCCATATGTACTCCCTATAAGAAAATTACTCATTGTTTAAAAAGAGATTTTATCTCTTAAGCGTAGTAATTTTAGCGGAGCCTGCCAGTTTCAGCTTACGCTTTTACTCTCATTTACGCTGCTGAGGCTGGTATATGTTGCATTTTTAGTTTAGAGTTTACTTAATTTAGAAAATACTTAAATAAATATGACTGAACTCGCGCA  >  NC_000913/2796974‑2797227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑