Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	355,227	C→A	G102G (GGC→GGA)	codB →	cytosine transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	355,227	0	C	A	100.0%	81.0 / NA	25	G102G (GGC→GGA)	codB	cytosine transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/11);  total (14/11)

ATCGGTAATCTTCTCCTCGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGT  >  NC_000913/355093‑355343                                                                                                                                       |                                                                                                                     aTCGGTAATCTTCTCCTCGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAAc                                                                                                                 >  1:399608/1‑140 (MQ=255)                ctcGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGtt                                                                                                  >  2:105131/1‑140 (MQ=255)                    gTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGc                                                                                              <  1:260107/140‑1 (MQ=255)                          acacTTCATTTCTCGGTTACATTGGCGCAAAAACTGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGgt                                                                                        <  1:299405/140‑1 (MQ=255)                               tCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGt                                                                                            >  2:66596/1‑131 (MQ=255)                               tCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGt                                                                                            <  1:66596/131‑1 (MQ=255)                                   ttCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATg                                                                               >  1:363403/1‑140 (MQ=255)                                          ttACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCa                                                                        >  1:90759/1‑140 (MQ=255)                                            aCATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCAtt                                                                      <  2:155878/140‑1 (MQ=255)                                             cATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTc                                                                     <  1:105131/140‑1 (MQ=255)                                                  gcgcAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCg                                                                   <  1:193246/137‑1 (MQ=255)                                                  gcgcAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCg                                                                   >  2:193246/1‑137 (MQ=255)                                                  gcgcAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTg                                                                >  1:214957/1‑140 (MQ=255)                                                                 tGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCggg                                                 <  1:264123/140‑1 (MQ=255)                                                                  gACCACCCACCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGc                                                <  2:252459/140‑1 (MQ=255)                                                                        tCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCATCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGata                                          >  1:155818/1‑140 (MQ=255)                                                                          aTCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATAtt                                        >  1:363122/1‑140 (MQ=255)                                                                                                      tAAAGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTAc            <  2:178267/140‑1 (MQ=255)                                                                                                        aaGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTTAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACtg          <  2:432213/140‑1 (MQ=255)                                                                                                        aaGGCTCATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACtg          >  2:316985/1‑140 (MQ=255)                                                                                                              cATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGttt                     >  1:71595/1‑123 (MQ=255)                                                                                                              cATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGttt                     <  2:71595/123‑1 (MQ=255)                                                                                                              cATGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGacc    >  1:189775/1‑140 (MQ=255)                                                                                                               aTGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGaccg   >  1:147590/1‑140 (MQ=255)                                                                                                               aTGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGaccg   >  2:371136/1‑140 (MQ=255)                                                                                                                tGGCTGCCTTCACTGCTACTGGGAGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGaccgt  <  1:100188/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                     ATCGGTAATCTTCTCCTCGGTATTTACACTTCATTTCTCGGTTACATTGGCGCAAAAACCGGCCTGACCACTCATCTTCTTGCTCGCTTCTCGTTTGGTGTTAAAGGCTCATGGCTGCCTTCACTGCTACTGGGCGGAACTCAGGTTGGCTGGTTTGGCGTCGGTGTGGCGATGTTTGCCATTCCGGTGGGTAAGGCAACCGGGCTGGATATTAATTTGCTGATTGCCGTTTCCGGTTTACTGATGACCGT  >  NC_000913/355093‑355343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑