Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,958,523	G→T	G185V (GGC→GTC)	ilvC →	ketol‑acid reductoisomerase (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,958,523	0	G	T	100.0%	98.3 / NA	30	G185V (GGC→GTC)	ilvC	ketol‑acid reductoisomerase (NADP(+))
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/13);  total (17/13)

CGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTG  >  NC_000913/3958389‑3958646                                                                                                                                       |                                                                                                                            cGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAg                                                                                                                        >  1:163595/1‑140 (MQ=255)         tCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCg                                                                                                                <  2:182119/140‑1 (MQ=255)          ccGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGa                                                                                                               <  2:444438/140‑1 (MQ=255)                  atatCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCaaa                                                                                                       >  2:58709/1‑140 (MQ=255)                  atatCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCaaa                                                                                                       >  2:338370/1‑140 (MQ=255)                   tatCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAg                                                                                                      >  1:379431/1‑140 (MQ=255)                    atCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGc                                                                                                     >  1:215101/1‑140 (MQ=255)                                aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                         >  1:26531/1‑140 (MQ=255)                                aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                         >  1:292722/1‑140 (MQ=255)                                aTGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGc                                                                                         <  1:338370/140‑1 (MQ=255)                                 tGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCa                                                                                        >  2:326387/1‑140 (MQ=255)                                       cgcCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggt                                                                                  >  2:361195/1‑140 (MQ=255)                                          cGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCggtggt                                                                               <  2:118536/140‑1 (MQ=255)                                                    aGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgg                                                                     <  1:361195/140‑1 (MQ=255)                                                    aGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgg                                                                     <  2:36945/140‑1 (MQ=255)                                                    aGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCgg                                                                     <  1:208533/140‑1 (MQ=255)                                                            aaGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGgtg                                                                  <  1:285686/135‑1 (MQ=255)                                                            aaGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGgtg                                                                  >  2:285686/1‑135 (MQ=255)                                                                  gTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCg                                                       >  2:337024/1‑140 (MQ=255)                                                                                    ggTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTg                                     >  2:56027/1‑140 (MQ=255)                                                                                         ggCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATc                                >  2:188313/1‑140 (MQ=255)                                                                                                   cGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCAACGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATg                      <  2:379431/140‑1 (MQ=255)                                                                                                        aTTGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGa                 <  2:292722/140‑1 (MQ=255)                                                                                                         ttGCCGTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAg                <  1:170404/140‑1 (MQ=255)                                                                                                              gTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATgg                     <  1:40038/130‑1 (MQ=255)                                                                                                              gTTCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATgg                     >  2:40038/1‑130 (MQ=255)                                                                                                               ttCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAAcc          <  1:56027/140‑1 (MQ=255)                                                                                                               ttCACCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAAcc          >  1:396409/1‑140 (MQ=255)                                                                                                                  aCCCGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATc       <  2:213043/140‑1 (MQ=255)                                                                                                                    ccGGAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCt     >  1:324437/1‑140 (MQ=255)                                                                                                                       gAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCtgtg  >  2:160937/1‑140 (MQ=255)                                                                                                                       gAAAACGATCCGAAAGTCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCtgtg  >  1:294030/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                            CGAGCAGATCCGTAAAGATATCACCGTAGTGATGGTTGCGCCGAAATGCCCAGGCACCGAAGTGCGTGAAGAGTACAAACGTGGGTTCGGCGTACCGACGCTGATTGCCGTTCACCCGGAAAACGATCCGAAAGGCGAAGGCATGGCGATTGCCAAAGCCTGGGCGGCTGCAACCGGTGGTCACCGTGCGGGTGTGCTGGAATCGTCCTTCGTTGCGGAAGTGAAATCTGACCTGATGGGCGAGCAAACCATCCTGTG  >  NC_000913/3958389‑3958646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑