| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,133,106 | C→A | A164E (GCG→GAG) | metF → | 5,10‑methylenetetrahydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,133,106 | 0 | C | A | 100.0% | 44.3 / NA | 14 | A164E (GCG→GAG) | metF | 5,10‑methylenetetrahydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (9/5); total (9/5) | |||||||||||
GGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTC > NC_000913/4132970‑4133240 | ggCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAggagga < 1:43906/140‑1 (MQ=255) tGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCtt > 1:444824/1‑140 (MQ=255) gggAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGa < 1:249867/140‑1 (MQ=255) tGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTAACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGTCa > 2:80656/1‑140 (MQ=255) cTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACcgc > 1:402966/1‑140 (MQ=255) ccTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGa < 1:176619/140‑1 (MQ=255) ggTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTa < 2:378765/140‑1 (MQ=255) gCTGTTAAAAGAAGTGGCAGATTTCGACATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGt > 1:353828/1‑140 (MQ=255) agaagTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCg < 1:291535/140‑1 (MQ=255) gCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGa > 1:363488/1‑140 (MQ=255) tGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGt > 1:120567/1‑140 (MQ=255) cggcgTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGAc < 2:444824/140‑1 (MQ=255) aaGCAAAAAGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGAt > 2:178351/1‑140 (MQ=255) aGCGCTCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAat > 2:72261/1‑140 (MQ=255) tCAGGAGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGGATTTTTGTGGGAATTTTTc > 1:297287/1‑140 (MQ=255) | GGCGATCTGCCGCCGGGAAGTGGTAAGCCAGAAATGTATGCTTCTGACCTGGTGACGCTGTTAAAAGAAGTGGCAGATTTCGATATCTCCGTGGCGGCGTATCCGGAAGTTCACCCGGAAGCAAAAAGCGCTCAGGCGGATTTGCTTAATCTGAAACGCAAAGTGGATGCCGGAGCCAACCGCGCGATTACTCAGTTCTTCTTCGATGTCGAAAGCTACCTGCGTTTTCGTGACCGCTGTGTATCGGCGGGCATTGATGTGGAAATTATTC > NC_000913/4132970‑4133240 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |