Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,492,306	G→T	L74M (CTG→ATG)	idnT ←	L‑idonate/5‑ketogluconate/gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,492,306	0	G	T	100.0%	101.5 / NA	30	L74M (CTG→ATG)	idnT	L‑idonate/5‑ketogluconate/gluconate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/20);  total (10/20)

CAAAACCCACTTCAAAAAACATGGCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACG  >  NC_000913/4492171‑4492442                                                                                                                                        |                                                                                                                                         cAAAACCCACTTCAAAAAACATGGCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTg                                                                                                                                      <  1:184829/140‑1 (MQ=255)                aaaaCATGGCGAGGCCCTCAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCg                                                                                                                       <  2:12929/140‑1 (MQ=255)                       ggCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATAGCCCCGAAACCGa                                                                                                                <  2:111339/140‑1 (MQ=255)                                  caACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGcc                                                                                                     <  2:245206/140‑1 (MQ=255)                                        gACCGGTGATCACTAGCGCCCATTGCGCGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccg                                                                                               >  2:277653/1‑140 (MQ=255)                                        gACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccg                                                                                               >  2:260929/1‑140 (MQ=255)                                         aCCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccgc                                                                                              >  1:189094/1‑140 (MQ=255)                                         aCCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccgc                                                                                              >  2:234738/1‑140 (MQ=255)                                         aCCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCccgc                                                                                              >  1:40620/1‑140 (MQ=255)                                                          cccATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             >  2:62735/1‑140 (MQ=255)                                                          cccATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             <  2:226715/140‑1 (MQ=255)                                                          cccATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             >  2:268795/1‑140 (MQ=255)                                                            cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  2:140904/140‑1 (MQ=255)                                                            cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  1:303158/140‑1 (MQ=255)                                                            cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  2:255016/140‑1 (MQ=255)                                                            cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  1:112375/140‑1 (MQ=255)                                                            cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCAGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  2:412484/140‑1 (MQ=255)                                                            cATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTCCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATAcc                                                                           <  1:378910/140‑1 (MQ=255)                                                             aTTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             <  2:191036/137‑1 (MQ=255)                                                             aTTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATa                                                                             >  1:191036/1‑137 (MQ=255)                                                              ttGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAt                                                                         <  1:62735/140‑1 (MQ=255)                                                              ttGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAt                                                                         <  2:338994/140‑1 (MQ=255)                                                               tGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCAtt                                                                        <  1:71923/140‑1 (MQ=255)                                                                      gTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGtata                                                                 <  2:85138/140‑1 (MQ=255)                                                                      gTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGtata                                                                 <  1:185042/140‑1 (MQ=255)                                                                               cAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAgg                                                        <  2:166165/140‑1 (MQ=255)                                                                                                 tAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGcatccatc                                      >  1:5705/1‑140 (MQ=255)                                                                                                                gtgCCGCACCCGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCcg                       >  2:158075/1‑140 (MQ=255)                                                                                                                         ccGTATCAGAAATCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCa              <  2:330798/140‑1 (MQ=255)                                                                                                                                     tCATCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACg  <  2:5705/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CAAAACCCACTTCAAAAAACATGGCGAGGCCCACAACCAGACCGGTGATCACTAGCGCCCATTGCACGCGTTTTTTACCAAAAGTAGCAATCAGCGTAGTGGCGATACGTTGTGCCGCACCCGTATCAGAAATCAGCTTGCCTAACATGGCCCCGAAACCGAGGATCATTGCCAGCCCGCCGAGCGTGCTGCCGATACCATTTTGTATAGAGTGCAGGACGGCCTGTGCATCCATCCCTTCGGCAAATCCGACGACGGCAGCTACCAGAACG  >  NC_000913/4492171‑4492442

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑