Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	365,410	G→T	P299Q (CCG→CAG)	lacZ ←	beta‑galactosidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	365,410	0	G	T	100.0%	34.0 / NA	12	P299Q (CCG→CAG)	lacZ	beta‑galactosidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/9);  total (3/9)

CAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCC  >  NC_000913/365280‑365539                                                                                                                                   |                                                                                                                                  cagACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGa                                                                                                                          <  1:96834/140‑1 (MQ=255)     ccATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGtt                                                                                                                      <  1:318778/140‑1 (MQ=255)                        cgcgGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGa                                                                                                   <  2:92372/140‑1 (MQ=255)                        cgcgGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGa                                                                                                   <  1:343597/140‑1 (MQ=255)                        cgcgGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGa                                                                                                   <  1:370424/140‑1 (MQ=255)                         gcgGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGAt                                                                                                  >  2:380755/1‑140 (MQ=255)                                                                                     aaCCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGa                                      >  1:290088/1‑140 (MQ=255)                                                                                      cccaccGCAGGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCCCGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGAc                                     <  2:67002/139‑1 (MQ=255)                                                                                       ccaccGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGAcc                                    >  2:299625/1‑140 (MQ=255)                                                                                        caccGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCt                                   <  1:299625/140‑1 (MQ=255)                                                                                                  tAGAGATTCGGGATTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCCCGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCAcc                         <  1:187579/140‑1 (MQ=255)                                                                                                  aagagATTGGGGTTTTCGGCGCTCCACAGTTTCTGTTTTTCGACGTTAAGACGTAGTGTGACGGGATCGGCATACCCCCCCCGCTCATCGATAATTCCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCAcc                         <  2:217707/139‑1 (MQ=255)                                                                                                   agagATTCGGGATTTCGGCGCTCCACAGTTTCTGTTTTTCGACGTTCAGACGTAGTGTGCCGCGATCGGCATACCCCCCCCGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCAccc                        <  2:268040/140‑1 (MQ=255)                                                                                                              aTTTCGGCGCTCCACAGTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGa             <  1:346680/140‑1 (MQ=255)                                                                                                                        cccACATTTTCTGGTTTTCGACGTTCAGACGTAGTGGGACGCGATGGGCATAACCCCCCCCCTCATCGATATTTTCCCCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCCCCCTGCCATAAAGAAACTGTTAcc   <  2:145337/139‑1 (MQ=255)                                                                                                                        cccACAGTTTCTGTTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTCCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTAcc   <  2:128425/139‑1 (MQ=255)                                                                                                                         ccACATTTTCTGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCAAACCCCCCCCGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGTGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTAccc  <  2:50829/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  CAGACCATTTTCAATCCGCACCTCGCGGAAACCGACATCGCAGGCTTCTGCTTCAATCAGCGTGCCGTCGGCGGTGTGCAGTTCAACCACCGCACGATAGAGATTCGGGATTTCGGCGCTCCACAGTTTCGGGTTTTCGACGTTCAGACGTAGTGTGACGCGATCGGCATAACCACCACGCTCATCGATAATTTCACCGCCGAAAGGCGCGGTGCCGCTGGCGACCTGCGTTTCACCCTGCCATAAAGAAACTGTTACCC  >  NC_000913/365280‑365539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑