| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 561,551 | C→A | Q619K (CAA→AAA) | sfmD → | putative fimbrial usher protein SfmD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 561,551 | 0 | C | A | 95.7% | 72.8 / ‑2.4 | 23 | Q619K (CAA→AAA) | sfmD | putative fimbrial usher protein SfmD |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base A (10/12); total (10/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.39e-01 | |||||||||||
ATCCCCGATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGG > NC_000913/561416‑561684 | aTCCCCGATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAaaaa > 1:436802/1‑140 (MQ=255) ccccGATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATa > 1:348295/1‑140 (MQ=255) acgaacgTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAgctggctg < 1:307480/140‑1 (MQ=255) acgaacgTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAgctggctg < 2:241466/140‑1 (MQ=255) acgaacgTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAgctggctg < 2:222156/140‑1 (MQ=255) cgaacgTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGctggctgg > 2:50223/1‑140 (MQ=255) cgaacgTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGctggctgg < 2:47633/140‑1 (MQ=255) gaacaTATTGTCGGACTTAATGTTTCAGTACCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTGCTTTGACGCCAACCGTAACAGCAACGGGAAAAATAGCtggctggc < 2:356795/140‑1 (MQ=255) aCTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGa < 2:31579/140‑1 (MQ=255) tAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCt < 2:436802/140‑1 (MQ=255) tGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGa > 2:196205/1‑140 (MQ=255) tGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGa > 2:251551/1‑140 (MQ=255) ttGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTa < 2:94577/140‑1 (MQ=255) ttGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTa > 1:176032/1‑140 (MQ=255) cAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACg < 2:198267/140‑1 (MQ=255) cAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCACTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACg < 2:310462/140‑1 (MQ=255) gggAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATAcc > 1:398155/1‑140 (MQ=255) gAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTc < 1:350859/140‑1 (MQ=255) aCGCCAACCGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAAc > 2:130226/1‑140 (MQ=255) cGTAACAGCAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGc < 2:348295/140‑1 (MQ=255) aaCAGCAACGGGAAAAATAGTTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGc < 2:252386/140‑1 (MQ=255) cAACGGGAAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACg > 2:20119/1‑140 (MQ=255) aaCGGGAAAAATAGCTGGCTGGCAGGAGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACgg > 2:257778/1‑140 (MQ=255) | ATCCCCGATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCAAAATAGCTGGCTGGCAGGTGTAGGTGGGACCTTACTGGAAGGCCACAACCTGAGTTATCACGTAAGCCAGGGTGATACCTCGAATAATGGGTACACGGGCAGCGCCACGGCAAACTGGCAGGCCGCTTACGG > NC_000913/561416‑561684 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |