Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	829,104	G→T	T290N (ACC→AAC)	ybhG ←	HlyD_D23 family protein YbhG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	829,104	0	G	T	100.0%	81.1 / NA	25	T290N (ACC→AAC)	ybhG	HlyD_D23 family protein YbhG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/14);  total (11/14)

TGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATA  >  NC_000913/828986‑829237                                                                                                                       |                                                                                                                                      tGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTcagcag                                                                                                                  >  2:201898/1‑140 (MQ=255)        tCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGcga                                                                                                           >  2:235823/1‑140 (MQ=255)          aCCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGcgaaa                                                                                                         <  2:295194/140‑1 (MQ=255)              aaTTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGcgaaacgaa                                                                                                     <  1:282046/140‑1 (MQ=255)                ttGTACCGTCACTGGCATTCCCTGGCGTAACGCATCACCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCgaaacgaaac                                                                                                   <  2:290562/140‑1 (MQ=255)                     ccGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATc                                                                                              >  2:320706/1‑140 (MQ=255)                                    ccTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGc                                                                               >  2:116521/1‑140 (MQ=255)                                             aCGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCggg                                                                      >  2:164425/1‑140 (MQ=255)                                             aCGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCggg                                                                      >  2:135851/1‑140 (MQ=255)                                                   catcGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCa                                                                <  2:31850/140‑1 (MQ=255)                                                    atcGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCAt                                                               <  2:9573/140‑1 (MQ=255)                                                    atcGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCAt                                                               <  1:397678/140‑1 (MQ=255)                                                    atcGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCAt                                                               <  1:235823/140‑1 (MQ=255)                                                         cGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCAt                                                               <  1:36480/135‑1 (MQ=255)                                                         cGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCAt                                                               >  2:36480/1‑135 (MQ=255)                                                         cGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGta                                                          <  1:433636/140‑1 (MQ=255)                                                           tCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGtata                                                        <  2:274231/140‑1 (MQ=255)                                                           tCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGtata                                                        >  1:135463/1‑140 (MQ=255)                                                                        tACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTg                                           >  1:19769/1‑140 (MQ=255)                                                                                      aCGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGc                             <  1:226327/140‑1 (MQ=255)                                                                                      aCGAGGTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCAGCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGc                             <  1:285583/140‑1 (MQ=255)                                                                                           gTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGt                        <  2:19769/140‑1 (MQ=255)                                                                                           gTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGt                        <  2:37430/140‑1 (MQ=255)                                                                                           gTCGGTACGCAGATCCGGCGTTTCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAACAACACTTTGCTCCCCGGCTGGCCCCGGt                        >  2:188001/1‑140 (MQ=255)                                                                                                                 tCGACGTTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATa  >  1:140565/1‑140 (MQ=255)                                                                                                                       |                                                                                                                                      TGCCTCGTCACCGAATTGTACCGTCACTGGCATTCCCTGGCGTAACGCATCATCGGCGTCGGTCACCACAATACGCAGGCGATAGACGAGGTCGGTACGCAGATCCGGCGTTTCGACGGTTTTCGGGGTAAATTCAGCAGTCGGCGAAACGAAACCAATCTGCCCGTGATACGGCTTGTCCGGGCGACCATCGGTATAAAGCAGCACTTTGCGCCCCGGCTGGGCCTGGTCAAGATTACGTTCATCAACATA  >  NC_000913/828986‑829237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑