Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,326,4500TG20.4% 111.0 / 14.3 44G114G (GGT→GGG) atoBacetyl‑CoA acetyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/35);  new base G (9/0);  total (9/35)
Fisher's exact test for biased strand distribution p-value = 1.41e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCAT  >  NC_000913/2326319‑2326579
                                                                                                                                   |                                                                                                                                 
ttAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGaaaa                                                                                                                           >  1:356379/1‑140 (MQ=255)
         gggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGttt                                                                                                                  <  2:171713/140‑1 (MQ=255)
          ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTTAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTa                                                                                                                 <  2:623863/140‑1 (MQ=255)
          ggCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTa                                                                                                                 <  1:199949/140‑1 (MQ=255)
            cTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGc                                                                                                               <  2:550328/140‑1 (MQ=255)
             tGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGcc                                                                                                              <  2:356379/140‑1 (MQ=255)
              ggCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGccc                                                                                                             <  1:385032/140‑1 (MQ=255)
                        gtgtGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCtacttact                                                                                                   <  2:590588/140‑1 (MQ=255)
                               gATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCa                                                                                            <  2:156443/140‑1 (MQ=255)
                                   cACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTTCTTACTCGATGCaaaaa                                                                                        >  1:261022/1‑139 (MQ=255)
                                    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGc                                                                                       <  1:309184/140‑1 (MQ=255)
                                    aCGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGc                                                                                       <  2:17636/140‑1 (MQ=255)
                                      ggTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCAc                                                                                     <  2:686754/140‑1 (MQ=255)
                                       gTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACg                                                                                    <  1:192381/140‑1 (MQ=255)
                                         cAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGct                                                                                  <  1:377156/140‑1 (MQ=255)
                                          aaTAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGctc                                                                                 <  2:277368/140‑1 (MQ=255)
                                            tAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTg                                                                               >  1:163312/1‑140 (MQ=255)
                                                gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTTCTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                           <  2:261022/140‑1 (MQ=255)
                                                gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                           <  2:101139/140‑1 (MQ=255)
                                                gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                           <  1:261364/140‑1 (MQ=255)
                                                gTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTa                                                                           <  2:612947/140‑1 (MQ=255)
                                                  aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGGAAATATGAGTTTTGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATc                                                                         >  1:48386/1‑140 (MQ=255)
                                                  aTGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCCACTTACTCGATGCAAAAGCACGCCCTGCTTAtt                                                                         >  2:352076/1‑139 (MQ=255)
                                                      ggTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGTTTGAAAATATTTGTTTGGCCCCCTACATATTCGTGGAAAAAGCAAGCTCTTGttttttgtt                                                                     >  2:446407/1‑133 (MQ=255)
                                                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgag                                                                <  1:370405/140‑1 (MQ=255)
                                                           gggTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGgag                                                                >  1:69628/1‑140 (MQ=255)
                                                                ttAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGa                                                           <  2:418709/140‑1 (MQ=255)
                                                                   aaaGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTCCTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAg                                                        <  2:719276/140‑1 (MQ=255)
                                                                                 gccgccCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGGTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAAt                                          >  1:496753/1‑140 (MQ=255)
                                                                                         ggCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACATAATCCTGcgcg                                  <  2:93789/140‑1 (MQ=255)
                                                                                          gCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGa                                 <  1:288918/140‑1 (MQ=255)
                                                                                           ccATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGAt                                <  1:13464/140‑1 (MQ=255)
                                                                                           ccATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGAt                                <  2:410579/140‑1 (MQ=255)
                                                                                           ccATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGCTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGAt                                <  1:352076/140‑1 (MQ=255)
                                                                                              ttCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGc                             <  2:69628/140‑1 (MQ=255)
                                                                                                   gcaggTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGAt                        <  2:99457/140‑1 (MQ=255)
                                                                                                      ggTCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGGCAGGGTTATGACGTAATCCTGCGCGATGGCCTGAtgtg                     >  2:201790/1‑140 (MQ=255)
                                                                                                        tCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTgcg                   <  2:48386/140‑1 (MQ=255)
                                                                                                        tCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCCGGGTATCGTCTTGGAGACGGACAGGGTTATGACGGAATCCTTCGCGATGGCCCGATGTgcg                   >  2:237078/1‑140 (MQ=255)
                                                                                                        tCAGGCGCAGAGCATTGTGGCGGGGGGGATGGAAAAAATGAGGTTTGCCCCCCACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGGTTTTGACGTAATCCTTCGCGATGGGCTGATGTgcg                   >  2:718855/1‑140 (MQ=255)
                                                                                                         cAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTgcgc                  <  1:397801/140‑1 (MQ=255)
                                                                                                         cAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTgcgc                  <  1:67657/140‑1 (MQ=255)
                                                                                                             cgcAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCAcc              <  1:540681/140‑1 (MQ=255)
                                                                                                               cAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCa            <  2:349265/140‑1 (MQ=255)
                                                                                                                  agCATTGTGGCGGGGGGGATGGAAAAAATGAGTTTAGCCCCCTACTTACTCGATGCAAAAACACGCTCTGGTTATCCTCTTTGAGACGGACAGGGTTATGACGTAATTCTGCGCGGTTGCCTGGTGTGCGCCACCCCTgg         >  2:110319/1‑140 (MQ=255)
                                                                                                                     aTTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTa      <  1:425356/140‑1 (MQ=255)
                                                                                                                         tgGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCat  <  2:624890/140‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                 
TTAAAAAGCGGGCTGGCAGAAACGGTGTGCGGATTCACGGTCAATAAAGTATGTGGTTCGGGTCTTAAAAGTGTGGCGCTTGCCGCCCAGGCCATTCAGGCAGGTCAGGCGCAGAGCATTGTGGCGGGGGGTATGGAAAATATGAGTTTAGCCCCCTACTTACTCGATGCAAAAGCACGCTCTGGTTATCGTCTTGGAGACGGACAGGTTTATGACGTAATCCTGCGCGATGGCCTGATGTGCGCCACCCATGGTTATCAT  >  NC_000913/2326319‑2326579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑