Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	38,537	G→T	G193G (GGC→GGA)	caiB ←	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	38,537	0	G	T	100.0%	151.2 / NA	44	G193G (GGC→GGA)	caiB	gamma‑butyrobetainyl‑CoA:carnitine CoA transferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/17);  total (27/17)

ACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTCTGAATCAGGTAAC  >  NC_000913/38405‑38670                                                                                                                                     |                                                                                                                                      aCCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCAAACATGGCGATGTCGATACTTTCTCCTTTAc                                                                                                                                >  2:593868/1‑140 (MQ=255)   cGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCg                                                                                                                              >  1:132521/1‑140 (MQ=255)   cGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCg                                                                                                                              >  2:153294/1‑140 (MQ=255)   cGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCACGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCg                                                                                                                              >  2:74997/1‑140 (MQ=255)               agtagGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACt                                                                                                                  <  2:334522/140‑1 (MQ=255)                   gggATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTAt                                                                                                              <  2:351487/140‑1 (MQ=255)                                ttGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAg                                                                                                 >  2:569938/1‑140 (MQ=255)                                  gCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAgcg                                                                                               >  1:738346/1‑140 (MQ=255)                                   cTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAgcgc                                                                                              >  2:491368/1‑140 (MQ=255)                                       tGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCgccgcc                                                                                          >  2:424783/1‑140 (MQ=255)                                        gcgcGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGccgccg                                                                                         <  1:10381/140‑1 (MQ=255)                                        gcgcGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGccgccg                                                                                         >  2:536099/1‑140 (MQ=255)                                                  aTTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGt                                                                               >  1:199324/1‑140 (MQ=255)                                                      cgccgcCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGAGGCGGTCAgg                                                                           >  2:607386/1‑140 (MQ=255)                                                            cgTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGaa                                                                     >  1:712790/1‑140 (MQ=255)                                                                      aTCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATcgg                                                           >  1:25869/1‑140 (MQ=255)                                                                      aTCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATcgg                                                           <  1:22050/140‑1 (MQ=255)                                                                       tCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATcggc                                                          >  2:172235/1‑140 (MQ=255)                                                                        ccATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATcggcg                                                         <  1:607386/140‑1 (MQ=255)                                                                           tcatGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGta                                                      >  1:696726/1‑140 (MQ=255)                                                                            catGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATcggcg                                                         <  2:481107/136‑1 (MQ=255)                                                                            catGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATcggcg                                                         >  1:481107/1‑136 (MQ=255)                                                                                 aGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACggg                                                <  1:536099/140‑1 (MQ=255)                                                                                 aGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACggg                                                >  2:189713/1‑140 (MQ=255)                                                                                      tGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTATTCGGCGGTATACGGGAaggc                                           >  2:668940/1‑140 (MQ=255)                                                                                      tGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAaggc                                           <  2:132521/140‑1 (MQ=255)                                                                                       ggCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAaggca                                          >  1:647617/1‑140 (MQ=255)                                                                                             tACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCAtt                                    >  1:311800/1‑140 (MQ=255)                                                                                                gcagcaTCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGc                                 <  2:25333/140‑1 (MQ=255)                                                                                                  agcaTCACTTCATCCATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTTCCCGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATGCGGGAAGGCAGGCATTGGCTg                               <  2:192709/140‑1 (MQ=255)                                                                                                  agcaTCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTg                               <  1:172235/140‑1 (MQ=255)                                                                                                  agcaTCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTg                               <  2:490785/140‑1 (MQ=255)                                                                                                  agcaTCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTg                               >  1:365056/1‑140 (MQ=255)                                                                                                    caTCACTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGt                             <  1:40767/140‑1 (MQ=255)                                                                                                         cTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACa                        >  1:271557/1‑140 (MQ=255)                                                                                                         cTTCATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACa                        >  1:505651/1‑140 (MQ=255)                                                                                                            cATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCa                     <  1:424783/140‑1 (MQ=255)                                                                                                            cATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCa                     <  2:365056/140‑1 (MQ=255)                                                                                                            cATACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCa                     <  1:392912/140‑1 (MQ=255)                                                                                                             aTACATGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCAc                    <  2:131898/140‑1 (MQ=255)                                                                                                                 aTGGCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGtt                <  1:636247/140‑1 (MQ=255)                                                                                                                   ggCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTCt              >  2:243956/1‑140 (MQ=255)                                                                                                                    gCGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTCTg             >  2:275044/1‑140 (MQ=255)                                                                                                                     cGATGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTCTGa            >  1:699780/1‑140 (MQ=255)                                                                                                                        tGTCGATACTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTCGGCAACCTCACCGTTCTGAAtt         >  2:539887/1‑139 (MQ=255)                                                                                                                               ccTTTCTCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCTCCGTTCTGAATCAGGTAAc  <  2:311800/139‑1 (MQ=255)                                                                                                                                     |                                                                                                                                      ACCGCAACCGGCGTAGTAGGGATCTTTACCTTTGCTCATGCGCGGGCACATTTCGCCGCCGTTGAAGTAATCCATCATGAAGTACTGGCCCATACGCAGCATCACTTCATACATGGCGATGTCGATACTTTCGCCTTTACCGGTTTCACGCACTTTATGCAGTGCTGCCAGCGCCGCCGTGGTGGCGGTCAGGCCAGAAAAGTAATCGGCGGTATACGGGAAGGCAGGCATTGGCTGGTCAACATCACCGTTCTGAATCAGGTAAC  >  NC_000913/38405‑38670

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑