Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,485,750	G→T	S459S (TCG→TCT)	evgS →	sensory histidine kinase EvgS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,485,750	0	G	T	100.0%	202.2 / NA	58	S459S (TCG→TCT)	evgS	sensory histidine kinase EvgS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (27/31);  total (27/31)

TTATGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAATAAAGCACTGAA  >  NC_000913/2485618‑2485884                                                                                                                                     |                                                                                                                                       ttatGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATGCAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACa                                                                                                                                 <  1:202247/140‑1 (MQ=255)    tGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATga                                                                                                                              <  1:255482/140‑1 (MQ=255)    tGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATga                                                                                                                              >  2:654928/1‑140 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  2:598740/140‑1 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  1:84356/140‑1 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  1:704767/140‑1 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  2:652127/140‑1 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  2:104804/140‑1 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  1:120198/140‑1 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  2:284766/140‑1 (MQ=255)     gAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgat                                                                                                                             <  1:419492/140‑1 (MQ=255)      aGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgatc                                                                                                                            >  2:65358/1‑140 (MQ=255)        cTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgatcga                                                                                                                          <  2:534139/140‑1 (MQ=255)         tGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgatcgat                                                                                                                         >  2:409072/1‑140 (MQ=255)         tGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATgatcgat                                                                                                                         <  1:536290/140‑1 (MQ=255)           cATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCa                                                                                                                       >  2:500566/1‑140 (MQ=255)                gCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACt                                                                                                                  >  1:686975/1‑140 (MQ=255)                        aaGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAAt                                                                                                          <  2:39474/140‑1 (MQ=255)                        aaGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAAt                                                                                                          <  2:686975/140‑1 (MQ=255)                            gaTGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAAc                                                                                                      >  1:720623/1‑140 (MQ=255)                             aTGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACt                                                                                                     <  1:567861/140‑1 (MQ=255)                                     ggAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCAtt                                                                                             <  2:490366/140‑1 (MQ=255)                                      gAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCAttt                                                                                            >  2:576926/1‑140 (MQ=255)                                       aGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCAtttt                                                                                           >  2:500873/1‑140 (MQ=255)                                       aGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCAtttt                                                                                           >  1:235841/1‑140 (MQ=255)                                           tGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTa                                                                                       >  2:742818/1‑140 (MQ=255)                                           tGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGCTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTa                                                                                       >  2:534953/1‑140 (MQ=255)                                            gAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTAt                                                                                      <  1:38900/140‑1 (MQ=255)                                                 gATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTg                                                                                 <  1:430351/140‑1 (MQ=255)                                                  aTACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTgg                                                                                <  1:500566/140‑1 (MQ=255)                                                   tACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGc                                                                               >  1:546658/1‑140 (MQ=255)                                                     cAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGt                                                                             <  2:463954/140‑1 (MQ=255)                                                       ggTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGCCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTc                                                                           >  2:239538/1‑140 (MQ=255)                                                           gATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGaa                                                                       >  1:71747/1‑140 (MQ=255)                                                            aTAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAAt                                                                      >  1:211244/1‑140 (MQ=255)                                                                tGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCAt                                                                  >  2:64805/1‑140 (MQ=255)                                                                tGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCAt                                                                  >  1:597246/1‑140 (MQ=255)                                                                    aGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCt                                                              >  2:55554/1‑140 (MQ=255)                                                                         tGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTttcgt                                                         >  2:542792/1‑140 (MQ=255)                                                                          gCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTttcgtt                                                        <  2:496396/140‑1 (MQ=255)                                                                                        tAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTcgcg                                          <  2:4149/140‑1 (MQ=255)                                                                                        tAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTcgcg                                          <  1:96298/140‑1 (MQ=255)                                                                                         aaggaaggTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCgg                                         <  1:654928/140‑1 (MQ=255)                                                                                               ggTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAAcc                                   <  2:442273/140‑1 (MQ=255)                                                                                               ggTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAAcc                                   <  1:275025/140‑1 (MQ=255)                                                                                               ggTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAAcc                                   <  2:274640/140‑1 (MQ=255)                                                                                                gTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCg                                  >  2:256809/1‑140 (MQ=255)                                                                                                 tGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCgg                                 >  2:64337/1‑140 (MQ=255)                                                                                                         aTGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAg                         >  1:88496/1‑140 (MQ=255)                                                                                                          tGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAgg                        <  2:720623/140‑1 (MQ=255)                                                                                                          tGCTCTGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAgg                        >  1:176966/1‑140 (MQ=255)                                                                                                            ctctGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGat                      <  1:88671/140‑1 (MQ=255)                                                                                                               tGGTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGAtatt                   >  1:240274/1‑140 (MQ=255)                                                                                                                ggTCGCGACACAGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGAtatta                  >  2:670466/1‑140 (MQ=255)                                                                                                                           aGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAATAAAGCa       <  2:546658/140‑1 (MQ=255)                                                                                                                           aGCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAATAAAGCa       >  1:416026/1‑140 (MQ=255)                                                                                                                            gCTAAATTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAATAAAGCAc      >  2:583254/1‑140 (MQ=255)                                                                                                                                aaTTCTCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAATAAAGCACTGaa  <  1:256469/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       TTATGAGCTGCATTCGCAATTAAAAGAGATGTATCCGGAGGTTGAATGGATACAGGTCGATAATGCCAGCGCTGCATTTCACAAGGTTAAGGAAGGTGAACTTGATGCTCTGGTCGCGACACAGCTAAATTCGCGTTACATGATCGATCATTACTATCCTAATGAACTTTATCATTTTCTTATTCCTGGCGTTCCGAATGCATCGCTTTCGTTCGCTTTTCCTCGCGGAGAACCGGAACTTAAGGATATTATTAATAAAGCACTGAA  >  NC_000913/2485618‑2485884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑