Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,951,529	G→T	G311G (GGC→GGA)	recD ←	exodeoxyribonuclease V subunit RecD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,951,529	0	G	T	100.0%	145.3 / NA	42	G311G (GGC→GGA)	recD	exodeoxyribonuclease V subunit RecD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (15/27);  total (15/27)

TGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCAC  >  NC_000913/2951395‑2951663                                                                                                                                       |                                                                                                                                       tGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAg                                                                                                                                   <  1:544355/140‑1 (MQ=255)     gcaggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGcaca                                                                                                                               <  1:138759/140‑1 (MQ=255)        ggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGcc                                                                                                                            <  2:229830/140‑1 (MQ=255)        ggcagAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGcc                                                                                                                            >  1:568447/1‑140 (MQ=255)               aCTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCt                                                                                                                     <  2:296160/140‑1 (MQ=255)                cTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTc                                                                                                                    >  2:171885/1‑140 (MQ=255)                  gTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCaa                                                                                                                  >  2:335303/1‑140 (MQ=255)                   tCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAAc                                                                                                                 >  1:550014/1‑140 (MQ=255)                   tCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAAc                                                                                                                 >  1:352979/1‑140 (MQ=255)                    cgcgCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAAcc                                                                                                                >  2:224006/1‑140 (MQ=255)                       gcAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAg                                                                                                             <  2:73041/140‑1 (MQ=255)                        cAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAgg                                                                                                            >  2:725188/1‑140 (MQ=255)                         aaaGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCGGCACAGCCCCAGCCTCAACCGAGGc                                                                                                           <  2:490115/140‑1 (MQ=255)                            gATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAg                                                                                                        <  1:382327/140‑1 (MQ=255)                            gATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAg                                                                                                        <  1:214182/140‑1 (MQ=255)                               gCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTg                                                                                                     >  2:260936/1‑140 (MQ=255)                                     tCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACg                                                                                               <  2:593598/140‑1 (MQ=255)                                     tCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACg                                                                                               <  1:98776/140‑1 (MQ=255)                                       tgtgCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGAt                                                                                             >  1:39407/1‑140 (MQ=255)                                          gCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCATCCTCAACCGAGGCCAGTTGATCACGATCGc                                                                                          <  2:550014/140‑1 (MQ=255)                                             ggTTCCGGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCga                                                                                       <  1:134946/140‑1 (MQ=255)                                                 ccTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGaaa                                                                                   <  1:479684/140‑1 (MQ=255)                                                   tGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGa                                                                                 >  1:609405/1‑140 (MQ=255)                                                       ggAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCAc                                                                             <  1:167845/140‑1 (MQ=255)                                                         aaCGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTc                                                                           >  2:580838/1‑140 (MQ=255)                                                                   cccGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATc                                                                 <  2:568447/140‑1 (MQ=255)                                                                        cAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCa                                                            <  1:279245/140‑1 (MQ=255)                                                                        cAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCa                                                            <  1:493579/140‑1 (MQ=255)                                                                              gcTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGt                                                      >  2:254248/1‑140 (MQ=255)                                                                                         tGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGtctc                                           <  1:224006/140‑1 (MQ=255)                                                                                          ggCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCg                                          <  1:634410/140‑1 (MQ=255)                                                                                            cACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGAc                                        <  1:533832/140‑1 (MQ=255)                                                                                                cTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGAcatca                                    <  1:580838/140‑1 (MQ=255)                                                                                                     cggTAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGc                               >  1:470541/1‑140 (MQ=255)                                                                                                        tAAAGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCGAGTTGATCACGGTCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAga                            <  2:736151/140‑1 (MQ=255)                                                                                                         aaaGCCCGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAgat                           <  1:514218/140‑1 (MQ=255)                                                                                                             cccGCGTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAgatcgat                       >  2:520924/1‑140 (MQ=255)                                                                                                               cgcgTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCa                     >  2:5470/1‑140 (MQ=255)                                                                                                                 cgTTGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCAtt                   <  2:352979/140‑1 (MQ=255)                                                                                                                    tGGCATAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGAc                <  2:545339/140‑1 (MQ=255)                                                                                                                        aTAAGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCtt            <  1:582305/140‑1 (MQ=255)                                                                                                                           aGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCAt         <  1:565491/140‑1 (MQ=255)                                                                                                                           aGCGCAGATATCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCAt         <  1:260936/140‑1 (MQ=255)                                                                                                                                  atatCTCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTaccac  <  2:343371/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       TGCAGCAGGCAGAGACTGTCGCGCAAAGATGCCGCTTCTGTGCCGGTTCCTGCCGGAACGTGAGTCCCCGTCAGGCGGCTTAGCTGCCTGGCACGCTCGGCGGTAAAGCCCGCGTTGGCATAAGCGCAGATATCGCCCAGCACAGCCCCAGCCTCAACCGAGGCCAGTTGATCACGATCGCCGAGAAAGATCACTCGCGCATGATCGGGCAAGGCGTCGATCAGTCTCGACATCATAGGCAGATCGATCATTGACGCTTCATCTACCAC  >  NC_000913/2951395‑2951663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑