Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	47,579	C→A	H112N (CAT→AAT)	kefF →	regulator of KefC‑mediated potassium transport and quinone oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	47,579	0	C	A	100.0%	178.0 / NA	51	H112N (CAT→AAT)	kefF	regulator of KefC‑mediated potassium transport and quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/32);  total (19/32)

TGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAAC  >  NC_000913/47444‑47709                                                                                                                                        |                                                                                                                                   tGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAAttt                                                                                                                                >  1:720106/1‑140 (MQ=255) tGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAAttt                                                                                                                                >  2:256722/1‑140 (MQ=255)      cAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGaaa                                                                                                                           >  1:21778/1‑140 (MQ=255)      cAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGaaa                                                                                                                           >  2:23811/1‑140 (MQ=255)           ttCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGt                                                                                                                      <  1:524048/140‑1 (MQ=255)           ttCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGt                                                                                                                      <  1:443000/140‑1 (MQ=255)           ttCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGt                                                                                                                      <  1:403785/140‑1 (MQ=255)           ttCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGt                                                                                                                      >  1:185095/1‑140 (MQ=255)           ttCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGt                                                                                                                      >  1:7285/1‑140 (MQ=255)           ttCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGt                                                                                                                      <  1:24415/140‑1 (MQ=255)              ctccGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTgcg                                                                                                                   >  2:312650/1‑140 (MQ=255)              ctccGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTgcg                                                                                                                   <  2:104623/140‑1 (MQ=255)                ccGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCa                                                                                                                 <  1:359188/140‑1 (MQ=255)                   ctcctcAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATcc                                                                                                              <  2:535010/140‑1 (MQ=255)                      ctcAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCggg                                                                                                           <  1:272529/140‑1 (MQ=255)                      ctcAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCggg                                                                                                           <  2:488703/140‑1 (MQ=255)                      ctcAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCggg                                                                                                           <  1:153246/140‑1 (MQ=255)                      ctcAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCggg                                                                                                           >  1:5097/1‑140 (MQ=255)                           aCTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGAAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTg                                                                                                      <  1:510012/140‑1 (MQ=255)                                ggATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGAtgtg                                                                                                 <  2:5097/140‑1 (MQ=255)                                       tAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGc                                                                                          >  2:45577/1‑140 (MQ=255)                                       tAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGc                                                                                          >  2:97958/1‑140 (MQ=255)                                        aaaGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCa                                                                                         <  1:280357/140‑1 (MQ=255)                                          aGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGc                                                                                       <  2:175712/140‑1 (MQ=255)                                                ctcCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTAc                                                                                 >  2:257399/1‑140 (MQ=255)                                                      cGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGa                                                                           >  1:366839/1‑140 (MQ=255)                                                      cGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGa                                                                           <  2:162782/140‑1 (MQ=255)                                                         cTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACgg                                                                        <  1:14495/140‑1 (MQ=255)                                                         cTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACgg                                                                        <  1:167027/140‑1 (MQ=255)                                                             gCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAAt                                                                    <  1:256722/140‑1 (MQ=255)                                                                 aCGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTAc                                                                >  2:399139/1‑140 (MQ=255)                                                                  cGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACt                                                               <  2:543348/140‑1 (MQ=255)                                                                   ggTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTg                                                              <  2:378389/140‑1 (MQ=255)                                                                      cATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCgg                                                           >  1:591844/1‑140 (MQ=255)                                                                       aTGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCggg                                                          >  1:563680/1‑140 (MQ=255)                                                                       aTGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCggg                                                          >  2:136615/1‑140 (MQ=255)                                                                        tGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGc                                                         >  2:377082/1‑140 (MQ=255)                                                                               aCGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTTGCAGCCGCTTCAGGCGACGGCAATCTACTGCGGGCTGAACTg                                                  >  2:338365/1‑140 (MQ=255)                                                                                    gcTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGc                                             <  2:720106/140‑1 (MQ=255)                                                                                    gcTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGc                                             <  1:230057/140‑1 (MQ=255)                                                                                        cATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCAcc                                         <  1:23811/140‑1 (MQ=255)                                                                                          tGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGt                                       <  1:45577/140‑1 (MQ=255)                                                                                          tGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGt                                       <  1:97958/140‑1 (MQ=255)                                                                                                  aTTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCAtg                               <  1:142511/140‑1 (MQ=255)                                                                                                     tGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGt                                       >  1:336399/1‑129 (MQ=255)                                                                                                     tGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGt                                       <  2:336399/129‑1 (MQ=255)                                                                                                      gCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCAtgcact                           <  2:709539/140‑1 (MQ=255)                                                                                                        tgtgGGCGGTGACGACCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCAtgcactgc                         <  2:21778/140‑1 (MQ=255)                                                                                                                    cgacCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTtgt             <  2:563680/140‑1 (MQ=255)                                                                                                                      acCGGCGGCGGGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTga           >  1:379712/1‑140 (MQ=255)                                                                                                                            ggcggGGAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGAcgacga     <  1:312650/140‑1 (MQ=255)                                                                                                                               ggggAAAGCAATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAAc  <  1:77244/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                   TGGTACAGCATTCCTCCGCTCCTCAAACTTTGGATCGATAAAGTTTTCTCCCACGGCTGGGCTTACGGTCATGGCGGCACGGCGCTGCATGGCAAACATTTGCTGTGGGCGGTGACGACCGGCGGCGGGGAAAGCCATTTTGAAATTGGTGCGCATCCGGGCTTTGATGTGCTGTCGCAGCCGCTACAGGCGACGGCAATCTACTGCGGGCTGAACTGGCTGCCACCGTTTGCCATGCACTGCACCTTTATTTGTGACGACGAAAC  >  NC_000913/47444‑47709

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑