Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,649,990	G→T	T352T (ACG→ACT)	arsB →	arsenite/antimonite:H(+) antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,649,990	0	G	T	100.0%	207.6 / NA	59	T352T (ACG→ACT)	arsB	arsenite/antimonite:H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (33/26);  total (33/26)

GTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACGGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACTAT  >  NC_000913/3649856‑3650124                                                                                                                                       |                                                                                                                                       gTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATc                                                                                                                                   >  1:216941/1‑140 (MQ=255) gTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATc                                                                                                                                   >  1:551381/1‑140 (MQ=255) gTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATc                                                                                                                                   >  1:376595/1‑140 (MQ=255)  tACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCt                                                                                                                                  >  2:684816/1‑140 (MQ=255)         cGTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTa                                                                                                                           >  1:376360/1‑140 (MQ=255)          gTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTAt                                                                                                                          <  2:688842/140‑1 (MQ=255)          gTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTAt                                                                                                                          <  2:237738/140‑1 (MQ=255)            gCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCa                                                                                                                        >  2:91446/1‑140 (MQ=255)             cTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCaa                                                                                                                       >  2:261214/1‑140 (MQ=255)               ggCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAaag                                                                                                                     >  1:698268/1‑140 (MQ=255)                 cGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAaagaa                                                                                                                   <  2:7672/140‑1 (MQ=255)                 cGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAaagaa                                                                                                                   >  2:550503/1‑140 (MQ=255)                     tAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGa                                                                                                               <  1:149942/140‑1 (MQ=255)                           ccTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGttt                                                                                                         >  1:128276/1‑140 (MQ=255)                              gtgGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATg                                                                                                      <  1:282679/140‑1 (MQ=255)                                     gcgACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAAtgt                                                                                               >  1:691793/1‑140 (MQ=255)                                           cTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTgg                                                                                         >  2:17377/1‑140 (MQ=255)                                                     gATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTg                                                                               <  1:601307/140‑1 (MQ=255)                                                                  cTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTa                                                                  <  1:236260/140‑1 (MQ=255)                                                                           ttctATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTg                                                         <  2:229532/140‑1 (MQ=255)                                                                            tctATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTgg                                                        >  2:408076/1‑140 (MQ=255)                                                                            tctATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTgg                                                        >  2:33123/1‑140 (MQ=255)                                                                                ttatGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    >  1:202986/1‑140 (MQ=255)                                                                                ttatGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    >  2:49717/1‑140 (MQ=255)                                                                                ttatGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    >  2:299711/1‑140 (MQ=255)                                                                                ttatGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    >  1:568299/1‑140 (MQ=255)                                                                                ttatGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    <  1:582295/140‑1 (MQ=255)                                                                                ttatGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    >  1:74693/1‑140 (MQ=255)                                                                                ttatGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGATAGc                                                    <  2:122462/140‑1 (MQ=255)                                                                                  atGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCt                                                  <  1:328712/140‑1 (MQ=255)                                                                                   tGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTg                                                 >  2:353083/1‑140 (MQ=255)                                                                                   tGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTg                                                 >  1:471037/1‑140 (MQ=255)                                                                                    gAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTgg                                                <  1:723116/140‑1 (MQ=255)                                                                                    gAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTgg                                                <  2:470952/140‑1 (MQ=255)                                                                                    gAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTgg                                                <  1:17377/140‑1 (MQ=255)                                                                                     aaCAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    >  1:419731/1‑135 (MQ=255)                                                                                     aaCAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGc                                                    <  2:419731/135‑1 (MQ=255)                                                                                     aaCAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGgc                                               >  2:743738/1‑140 (MQ=255)                                                                                     aaCAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGgc                                               >  2:427339/1‑140 (MQ=255)                                                                                         atatGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGgctacg                                           <  1:261214/140‑1 (MQ=255)                                                                                         atatGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGgctacg                                           <  1:46927/140‑1 (MQ=255)                                                                                             gCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGgctacgctac                                       >  1:412300/1‑140 (MQ=255)                                                                                              ccGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTAct                                      <  1:500487/140‑1 (MQ=255)                                                                                               cGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTActc                                     <  1:299711/140‑1 (MQ=255)                                                                                                 aCGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTg                                   <  2:407040/140‑1 (MQ=255)                                                                                                      aCTGGTTGGCGCGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGc                              >  1:322299/1‑140 (MQ=255)                                                                                                              gcgcGTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACtt                      >  2:555650/1‑140 (MQ=255)                                                                                                               cgcgTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACttt                     >  2:239581/1‑140 (MQ=255)                                                                                                               cgcgTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACttt                     >  1:606597/1‑140 (MQ=255)                                                                                                                  gTTGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGc                  <  2:51090/140‑1 (MQ=255)                                                                                                                    tGTCCATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCag                >  1:489519/1‑140 (MQ=255)                                                                                                                       ccATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCagaag             <  1:323542/140‑1 (MQ=255)                                                                                                                        cATTGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCagaaga            <  1:550503/140‑1 (MQ=255)                                                                                                                           tGATGGCAGCACTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAata         <  1:698303/140‑1 (MQ=255)                                                                                                                                gcagcaCTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACt    <  2:471037/140‑1 (MQ=255)                                                                                                                                gcagcaCTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACt    >  2:428917/1‑140 (MQ=255)                                                                                                                                gcagcaCTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACt    >  2:370459/1‑140 (MQ=255)                                                                                                                                 cagcaCTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACTa   >  2:478710/1‑140 (MQ=255)                                                                                                                                  agcaCTGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACTAt  <  1:33123/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       GTACTCAACGTGCTGGCGGATAACGGCCTGTGGGCCGCGACGCTCGGCACCGGATTCCTCACCGCCTTCCTCTCTTCTATTATGAACAATATGCCGACGGTACTGGTTGGCGCGTTGTCCATTGATGGCAGCACGGCATCTGGCGTTATCAAAGAAGCGATGGTTTATGCCAATGTGATTGGCTGCGATTTGGGACCGAAAATTACCCCAATTGGTAGCCTGGCTACGCTACTCTGGCTGCACGTACTTTCGCAGAAGAATATGACTAT  >  NC_000913/3649856‑3650124

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑