Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,030,852	G→T	G40G (GGC→GGA)	fadB ←	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,030,852	0	G	T	100.0%	137.9 / NA	40	G40G (GGC→GGA)	fadB	dodecenoyl‑CoA delta‑isomerase, enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, 3‑hydroxyacyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/21);  total (19/21)

TTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCC  >  NC_000913/4030719‑4030988                                                                                                                                      |                                                                                                                                         ttAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAgg                                                                                                                                    >  1:51191/1‑140 (MQ=255)       gTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCg                                                                                                                              >  2:494039/1‑140 (MQ=255)         tcttcAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGAc                                                                                                                            >  1:491795/1‑140 (MQ=255)                gAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCa                                                                                                                     <  1:55386/140‑1 (MQ=255)                  aCGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAgt                                                                                                                   >  1:198571/1‑140 (MQ=255)                   cGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAgtg                                                                                                                  <  1:646652/140‑1 (MQ=255)                    gAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAgtgt                                                                                                                 <  2:598889/140‑1 (MQ=255)                     aGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTc                                                                                                                >  1:703687/1‑140 (MQ=255)                      ggAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCg                                                                                                               <  1:368001/140‑1 (MQ=255)                          cAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGtt                                                                                                           >  2:192897/1‑140 (MQ=255)                           aGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGttt                                                                                                          <  2:570992/140‑1 (MQ=255)                             ggACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTttat                                                                                                        <  2:47937/140‑1 (MQ=255)                                 aaaaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAAc                                                                                                    >  1:406516/1‑140 (MQ=255)                                  aaaaTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACt                                                                                                   <  2:498373/140‑1 (MQ=255)                                     aTTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGaa                                                                                                >  1:553814/1‑140 (MQ=255)                                           tGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTggg                                                                                          >  1:402433/1‑140 (MQ=255)                                           tGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTggg                                                                                          <  2:1704/140‑1 (MQ=255)                                            gATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTgggg                                                                                         <  1:129461/140‑1 (MQ=255)                                              tatCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCa                                                                                       <  1:443879/140‑1 (MQ=255)                                               atCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCAt                                                                                      >  1:410284/1‑140 (MQ=255)                                                         acgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAGCCTGGGGCATCAAATACCAg                                                                            >  2:1501/1‑140 (MQ=255)                                                         acgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAg                                                                            >  1:557471/1‑140 (MQ=255)                                                         acgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAg                                                                            >  1:274553/1‑140 (MQ=255)                                                          cgaTAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGt                                                                           <  2:553814/140‑1 (MQ=255)                                                              aaaGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCgg                                                                       <  1:569952/140‑1 (MQ=255)                                                              aaaGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCgg                                                                       <  2:733448/140‑1 (MQ=255)                                                                   ctgctTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATg                                                                  <  2:39467/140‑1 (MQ=255)                                                                     gctTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGcc                                                                <  2:59196/140‑1 (MQ=255)                                                                                aCGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTccagcc                                                     <  1:192897/140‑1 (MQ=255)                                                                                  gcagcagcaGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATCCCAGTTCGGCAATGCCATCTTccagccag                                                   <  2:23959/140‑1 (MQ=255)                                                                                     gcagcagcCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCa                                                <  1:258844/140‑1 (MQ=255)                                                                                              cTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAgg                                       >  2:109751/1‑140 (MQ=255)                                                                                                     aTCTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGc                                <  1:494039/140‑1 (MQ=255)                                                                                                       cTGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCt                              >  1:80350/1‑140 (MQ=255)                                                                                                        tGATTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACATGGTGTCGCCtt                             <  1:473362/140‑1 (MQ=255)                                                                                                          aTTGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTg                           >  2:736925/1‑140 (MQ=255)                                                                                                            tGCTGTTCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTa                         <  2:410284/140‑1 (MQ=255)                                                                                                                 ttCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCa                    >  1:713586/1‑140 (MQ=255)                                                                                                                 ttCCAGCACGCCGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCa                    >  2:649214/1‑140 (MQ=255)                                                                                                                           ccGATGGCCTCTCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGtctc          >  1:595717/1‑140 (MQ=255)                                                                                                                                   ctctCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGCGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATcc  <  2:703687/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         TTAACTGTTCTTCAGGAACGAGGAACAGGGACAAAAATTCGGTGATATCAGCACCGACGATAAAGGCTGCTTTGTTCGAACGCAGCAGCAGCCCTTTTAGATCTGATTGCTGTTCCAGCACGCCGATGGCCTCGCCGAGGCTGGCGACGGTCGCAGTGTCGAGTTTATTAACTGAACCTGGGGCATCAAATACCAGTTCGGCAATGCCATCTTCCAGCCAGTCAAGGTACAGGGTGTCGCCTTTGTAAAGCATGTCAGTCTCCTGAATCC  >  NC_000913/4030719‑4030988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑