Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	79,715	C→A	A384S (GCC→TCC)	leuC ←	3‑isopropylmalate dehydratase subunit LeuC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	79,715	0	C	A	100.0%	109.8 / NA	32	A384S (GCC→TCC)	leuC	3‑isopropylmalate dehydratase subunit LeuC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (13/19);  total (13/19)

CTGGTGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTG  >  NC_000913/79582‑79845                                                                                                                                      |                                                                                                                                   cTGGTGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTAc                                                                                                                              >  2:92809/1‑140 (MQ=255)  tggtggAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTAcc                                                                                                                             >  1:43207/1‑140 (MQ=255)            acaACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGc                                                                                                                   <  1:182217/140‑1 (MQ=255)              aaCGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCg                                                                                                                 <  2:492836/140‑1 (MQ=255)               aCGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCgg                                                                                                                >  2:731470/1‑140 (MQ=255)                cGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCggg                                                                                                               <  2:605520/140‑1 (MQ=255)                cGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCggg                                                                                                               <  2:152470/140‑1 (MQ=255)                  ttCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGaa                                                                                                             <  2:679132/140‑1 (MQ=255)                      cccGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAaccac                                                                                                         <  1:99844/140‑1 (MQ=255)                           aTTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTg                                                                                                    <  1:336312/140‑1 (MQ=255)                                    gTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGc                                                                                           >  1:613796/1‑140 (MQ=255)                                          gttCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGgcg                                                                                     >  2:715256/1‑140 (MQ=255)                                               tCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACt                                                                                >  2:676191/1‑140 (MQ=255)                                                   cAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTc                                                                            >  2:87001/1‑140 (MQ=255)                                                     gACACCTTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGc                                                                          >  2:727496/1‑140 (MQ=255)                                                     gACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACTGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGc                                                                          >  1:231027/1‑140 (MQ=255)                                                      acacATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGcc                                                                         <  2:265428/140‑1 (MQ=255)                                                           ttGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTg                                                                    <  2:501861/140‑1 (MQ=255)                                                                          aGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGcc                                                     <  1:522766/140‑1 (MQ=255)                                                                           gcgcCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCcg                                                    <  1:87001/140‑1 (MQ=255)                                                                             gcCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCcgcg                                                  >  1:48485/1‑140 (MQ=255)                                                                                 ttCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTa                                              >  1:271109/1‑140 (MQ=255)                                                                                       aCCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCtt                                        <  2:728482/140‑1 (MQ=255)                                                                                              tCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATgcg                                 <  1:412402/140‑1 (MQ=255)                                                                                                    aaGATTTTATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGtt                           <  1:735822/140‑1 (MQ=255)                                                                                                          ttATCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACa                     >  2:1149/1‑140 (MQ=255)                                                                                                            aTCCAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAgg                   >  1:213817/1‑140 (MQ=255)                                                                                                              ccAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGaa                 <  2:613796/140‑1 (MQ=255)                                                                                                              ccAGACCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGaa                 <  1:53834/140‑1 (MQ=255)                                                                                                                  aCCTTCCGCTTCCGCCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGa             <  1:518594/140‑1 (MQ=255)                                                                                                                            tccgcCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATaa          <  2:539955/133‑1 (MQ=255)                                                                                                                            tccgcCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATaa          >  1:539955/1‑133 (MQ=255)                                                                                                                             ccgcCTGGGATTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCCGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTg  <  1:92358/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                   CTGGTGGAGGCACAACGTTCGCCCGGATTCAGACGGTCGTTGTTCATCGCCAGACACATTGAGCAGCCAGGCAAGCGCCATTCAAAACCGGCTTCAATAAAGATTTTATCCAGACCTTCCGCTTCCGCCTGGGCTTTTACCGGGCCAGAGCCGGGAACCACCAGTGCCTGCACGCCTGGCGCGACTTTTCGCCCTTTGGCGATCTCCGCTGCCGCGCGTAAATCTTCAATGCGCGAGTTGGTACAGGAACCGATAAACACTTTG  >  NC_000913/79582‑79845

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑