Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 549,560 C→A L402M (CTG→ATG)  ylbE → DUF1116 domain‑containing protein YlbE

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913549,5600CA95.8% 76.4 / ‑2.4 24L402M (CTG→ATG) ylbEDUF1116 domain‑containing protein YlbE
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (7/16);  total (7/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

GCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGG  >  NC_000913/549435‑549696
                                                                                                                             |                                                                                                                                        
gcgcGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGaa                                                                                                                            <  1:262109/140‑1 (MQ=255)
           gggCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGa                                                                                                                 <  1:233196/140‑1 (MQ=255)
             gCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAg                                                                                                               <  2:455253/140‑1 (MQ=255)
                ggACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCAc                                                                                                            <  1:35491/140‑1 (MQ=255)
                     ttCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCt                                                                                                       <  1:631781/140‑1 (MQ=255)
                      tcgtcgCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGGTCGGGCAGATTGGCGCAGGCACCCGGCGGGCACCGATGGCGGGCTTTTAACAGGCGCTGGGAGCACTGGCTg                                                                                                      >  2:519507/1‑140 (MQ=255)
                            cgTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCa                                                                                                <  2:238331/140‑1 (MQ=255)
                            cgTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCa                                                                                                <  2:267421/140‑1 (MQ=255)
                             gTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCAt                                                                                               <  1:640058/140‑1 (MQ=255)
                                   gAAGCCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTAt                                                                                         <  2:660459/140‑1 (MQ=255)
                                   gAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTAt                                                                                         <  1:615063/140‑1 (MQ=255)
                                      aCCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTgg                                                                                      <  1:150638/140‑1 (MQ=255)
                                        cGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGtt                                                                                    <  2:253170/140‑1 (MQ=255)
                                             ttACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGa                                                                               <  2:4150/140‑1 (MQ=255)
                                                cGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAAGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAcg                                                                            >  1:94050/1‑140 (MQ=255)
                                                  ccACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAAcgcg                                                                          <  2:490866/140‑1 (MQ=255)
                                                            aTACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGat                                                                <  1:7326/140‑1 (MQ=255)
                                                                  gTATCGCCCATAAAGAGGCGGGGGTCGGGCAGATTGGCGCCGGCACCCGGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGGAGCACTGGCTGAAAGCATGGGGGTTGGGTGAGGGACGCGCAAAGACGATcatccc                                                          >  2:317689/1‑139 (MQ=255)
                                                                            tAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGGACCGTGCGGGCACCGATGGCGCGCTTTGAACAGGCGCTGGGAGAACTGGCTGGAAGCATGGGGATTGGGTGAGGAAAGCGCCAAGACGATCATCCATCCTCTGCtt                                                >  2:549131/1‑140 (MQ=255)
                                                                                   gCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGATGGCGGGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGGATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCagta                                         >  2:487898/1‑140 (MQ=255)
                                                                                                  aTTGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAAtt                          >  1:498165/1‑140 (MQ=255)
                                                                                                    tGGCGCAGGCACCGTGCGGGCACCGATGGTATGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTAt                        >  1:287171/1‑140 (MQ=255)
                                                                                                    tGGCGCAGGCACCGTGCGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTAt                        >  2:378067/1‑140 (MQ=255)
                                                                                                                    cGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTgg            >  2:454154/1‑136 (MQ=255)
                                                                                                                    cGGGCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTgg            <  1:454154/136‑1 (MQ=255)
                                                                                                                      ggCACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTa      >  2:513816/1‑140 (MQ=255)
                                                                                                                        cACCGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGc    <  1:241599/140‑1 (MQ=255)
                                                                                                                          ccGATGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGCATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCgg  >  1:239357/1‑140 (MQ=255)
                                                                                                                             |                                                                                                                                        
GCGCGTGCCTGGGGCTGGACATTCGTCGCGTGGTAGAAACCGGCATTACGCCACTCATCAATACCGGTATCGCCCATAAAGAGGCGGGGATCGGGCAGATTGGCGCAGGCACCGTGCGGGCACCGCTGGCGTGCTTTGAACAGGCGCTGGAAGCACTGGCTGAAAGCATGGGTATTGGTTGAGGAACGCGCAATGACGATCATCCATCCTCTGCTTGCCAGTAGTAGCGCACCGAATTATCGCCAGTCCTGGCGGTTAGCGG  >  NC_000913/549435‑549696

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑