Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	671,960	C→A	R76L (CGT→CTT)	lptE ←	lipopolysaccharide assembly protein LptE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	671,960	0	C	A	100.0%	173.9 / NA	50	R76L (CGT→CTT)	lptE	lipopolysaccharide assembly protein LptE
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/26);  total (24/26)

GGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAG  >  NC_000913/671827‑672092                                                                                                                                      |                                                                                                                                     ggCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAgg                                                                                                                                <  2:501445/140‑1 (MQ=255)      tAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGa                                                                                                                           >  1:289977/1‑140 (MQ=255)       aaTCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGaa                                                                                                                          <  1:391558/140‑1 (MQ=255)                       cGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGt                                                                                                          >  1:15635/1‑140 (MQ=255)                           cGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCt                                                                                                      >  1:627990/1‑140 (MQ=255)                                   aaCACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAg                                                                                              <  2:87425/140‑1 (MQ=255)                                    acacGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGc                                                                                             >  2:624059/1‑140 (MQ=255)                                        ggTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACt                                                                                         >  2:644745/1‑140 (MQ=255)                                          tCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCg                                                                                       >  1:29814/1‑140 (MQ=255)                                          tCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCg                                                                                       >  2:648426/1‑140 (MQ=255)                                          tCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCg                                                                                       >  2:67600/1‑140 (MQ=255)                                             cATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                                                                                    <  1:279948/140‑1 (MQ=255)                                             cATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGaca                                                                                    >  2:72015/1‑140 (MQ=255)                                                  aCCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCAtt                                                                               <  1:289482/140‑1 (MQ=255)                                                      tCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGa                                                                           >  1:424111/1‑140 (MQ=255)                                                            tcatcTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAc                                                                     <  2:327061/140‑1 (MQ=255)                                                            tcatcTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAc                                                                     <  2:424111/140‑1 (MQ=255)                                                            tcatcTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAAc                                                                     <  1:159402/140‑1 (MQ=255)                                                                 tGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGtt                                                                >  1:20051/1‑140 (MQ=255)                                                                 tGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCAC‑TTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTa                                                               >  1:201204/1‑140 (MQ=255)                                                                   aTACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTGTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAc                                                              >  2:668571/1‑140 (MQ=255)                                                                    tACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcg                                                             >  2:642633/1‑140 (MQ=255)                                                                         tgctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcaccg                                                        >  1:610932/1‑140 (MQ=255)                                                                         tgctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcaccg                                                        >  1:195879/1‑140 (MQ=255)                                                                           ctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcaccgca                                                      >  1:449487/1‑140 (MQ=255)                                                                           ctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcaccgca                                                      <  1:124224/140‑1 (MQ=255)                                                                           ctgTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTAcgcaccgca                                                      <  2:29814/140‑1 (MQ=255)                                                                             gTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACg                                                    <  1:351656/140‑1 (MQ=255)                                                                                     gTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATg                                            >  1:190308/1‑140 (MQ=255)                                                                                        aCGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGcc                                         >  2:560403/1‑140 (MQ=255)                                                                                                 cGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGAt                                >  1:722830/1‑140 (MQ=255)                                                                                                   aTGCGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCg                              >  1:255602/1‑140 (MQ=255)                                                                                                     gCGGTATCTTTCGCGATGCTCAC‑TTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCt                           <  2:201204/140‑1 (MQ=255)                                                                                                      cGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCt                           <  2:627990/140‑1 (MQ=255)                                                                                                      cGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCt                           <  1:644745/140‑1 (MQ=255)                                                                                                      cGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCt                           >  2:604729/1‑140 (MQ=255)                                                                                                      cGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCt                           <  2:20051/140‑1 (MQ=255)                                                                                                      cGGTATCTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCt                           <  2:441876/140‑1 (MQ=255)                                                                                                      cGGTATCTTTCGCGATGCTCACTTCACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCt                           <  1:316787/140‑1 (MQ=255)                                                                                                            cTTTCGCGATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTcc                     <  2:289977/140‑1 (MQ=255)                                                                                                                   gATGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCa              >  2:572696/1‑140 (MQ=255)                                                                                                                    aTGCTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCAt             <  2:668360/140‑1 (MQ=255)                                                                                                                       cTCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGAc          <  1:390007/140‑1 (MQ=255)                                                                                                                        tCACTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGAcc         <  1:648426/140‑1 (MQ=255)                                                                                                                           cTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTc      <  2:449487/140‑1 (MQ=255)                                                                                                                           cTTTACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTc      <  2:15635/140‑1 (MQ=255)                                                                                                                            tttACCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCa     <  2:190308/140‑1 (MQ=255)                                                                                                                               aCCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAg  >  2:16373/1‑140 (MQ=255)                                                                                                                               aCCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAg  <  1:72015/140‑1 (MQ=255)                                                                                                                               aCCCAAAAGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAg  <  1:624059/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GGCGCTAATCGGGTAGATATCACGGCCGGGGATCAACACGGTCGCATTAACCGTCATGATCATCTGATACTCTGCTGTTTGACCGTTACGGAATACCGATGCGGTATCTTTCGCGATGCTCACTTTACCCAAACGCAAGGATGGAACGTCCTTACGCGTGGTTTCTTTATCAAGCAACTCGACACCATTCAGACGTAACTGGTTACGCACCGCACGGCTTAATGGCCCGTTCGGATCGCCTGAGTCCAGGATCATGACCTTCATAG  >  NC_000913/671827‑672092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑