Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	963,290	C→A	G432G (GGC→GGA)	rpsA →	30S ribosomal subunit protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	963,290	0	C	A	100.0%	116.5 / NA	34	G432G (GGC→GGA)	rpsA	30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/18);  total (16/18)

CTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGCGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGGCGCAACCGTAGAACTGGCTGACGGCGTTGAAGG  >  NC_000913/963162‑963421                                                                                                                                 |                                                                                                                                    cTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCt                                                                                                                          <  2:332133/140‑1 (MQ=255) cTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCt                                                                                                                          <  2:365134/140‑1 (MQ=255)     ttCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCa                                                                                                                      >  1:547153/1‑140 (MQ=255)              cTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCagaag                                                                                                                  <  2:323282/135‑1 (MQ=255)              cTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCagaag                                                                                                                  >  1:323282/1‑135 (MQ=255)                   aTCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTcaa                                                                                                        >  1:214434/1‑140 (MQ=255)                       ccTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCaacaac                                                                                                    >  2:583725/1‑140 (MQ=255)                       ccTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCaacaac                                                                                                    >  2:331673/1‑140 (MQ=255)                       ccTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCaacaac                                                                                                    >  1:360981/1‑140 (MQ=255)                       ccTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCaacaac                                                                                                    >  2:42314/1‑140 (MQ=255)                           gAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTggg                                                                                                <  2:703744/140‑1 (MQ=255)                           gAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTggg                                                                                                >  1:365028/1‑140 (MQ=255)                           gAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTggg                                                                                                <  1:472361/140‑1 (MQ=255)                            aaCGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGt                                                                                               >  2:384594/1‑140 (MQ=255)                                ttGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGct                                                                                           >  1:241067/1‑140 (MQ=255)                                ttGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGct                                                                                           <  1:669231/140‑1 (MQ=255)                                ttGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGct                                                                                           >  1:662642/1‑140 (MQ=255)                                ttGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGct                                                                                           <  1:339045/140‑1 (MQ=255)                                           gaagCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGaa                                                                                >  1:396613/1‑140 (MQ=255)                                                aGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGgcg                                                                           <  2:73265/140‑1 (MQ=255)                                                aGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGgcg                                                                           <  2:194087/140‑1 (MQ=255)                                                aGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGgcg                                                                           <  1:717203/140‑1 (MQ=255)                                                       gAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGt                                                                    <  2:324456/140‑1 (MQ=255)                                                              aaaaaGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGt                                                             <  2:547153/140‑1 (MQ=255)                                                                              cGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTg                                             <  2:396613/140‑1 (MQ=255)                                                                              cGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGGAACTGCAGTTg                                             <  1:331673/140‑1 (MQ=255)                                                                               gCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGa                                            <  2:662642/140‑1 (MQ=255)                                                                                          tCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGgcg                                 <  1:270148/140‑1 (MQ=255)                                                                                           cTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTTAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGgcgc                                >  2:292432/1‑140 (MQ=255)                                                                                                ggTTGACGCAGAACGTGAACGTATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGGCGCAACCg                           <  1:384594/140‑1 (MQ=255)                                                                                                              gtgaacgtATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGGCGCAACCGTAGAACTGGCTGAc             <  2:300420/140‑1 (MQ=255)                                                                                                                gaacgtATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGGCGCAACCGTAGAACTGGCTGACgg           >  1:315921/1‑140 (MQ=255)                                                                                                                     tATCTCCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGGCGCAACCGTAGAACTGGCTGACGGCGTTg      >  2:472035/1‑140 (MQ=255)                                                                                                                         tcCCTGGGAGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGGCGCAACCGTAGAACTGGCTGACGGCGTTGAAgg  >  2:61031/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                                    CTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCTGGGCGTTAAACAGCTCGCAGAAGATCCGTTCAACAACTGGGTTGCTCTGAACAAGAAAGGCGCTATCGTAACCGGTAAAGTAACTGCAGTTGACGCTAAAGGCGCAACCGTAGAACTGGCTGACGGCGTTGAAGG  >  NC_000913/963162‑963421

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑