Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	173,109	C→A	L550M (CTG→ATG)	fhuB →	iron(III) hydroxamate ABC transporter membrane subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	173,109	0	C	A	100.0%	65.7 / NA	21	L550M (CTG→ATG)	fhuB	iron(III) hydroxamate ABC transporter membrane subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/9);  total (12/9)

TGACCTGGATTTCCGGTTCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGCTGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGACCGTTGAGTTTTGTTGGTTTAATGGC  >  NC_000913/172987‑173242                                                                                                                           |                                                                                                                                      tGACCTGGATTTCCGGTTCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGcc                                                                                                                      <  1:427751/140‑1 (MQ=255)   aCCTGGATTTCCGGTTCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCg                                                                                                                    <  2:495226/140‑1 (MQ=255)      tGGATTTCCGGTTCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGc                                                                                                                 <  1:548788/140‑1 (MQ=255)        gATTTCCGGTTCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCg                                                                                                               >  1:402253/1‑140 (MQ=255)         aTTTCCGGTTCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGt                                                                                                              <  2:712491/140‑1 (MQ=255)         aTTTCCGGTTCGACCTAAACGGCGCCCGTGGCGGGGGTCGGGCGCCCCGAAATTGTGATGGTGATTTTGCTGGCAATTCCCCCGCTGTGCGCCCGCTGGTTGACCATTTTACCGATGGGGGGTATTACCGCCCGAGCCGt                                                                                                              <  2:553585/140‑1 (MQ=255)                  tCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGgc                                                                                                     >  1:278894/1‑140 (MQ=255)                                gACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGAcgc                                                                                       >  1:803250/1‑140 (MQ=255)                                 aCCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGAcgcg                                                                                      >  2:98204/1‑140 (MQ=255)                                     aTGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAAtt                                                                                  >  2:705863/1‑140 (MQ=255)                                        cgcAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTgcg                                                                               <  1:705863/140‑1 (MQ=255)                                           aGGTCTGGCGCCCCGGAATTGTGATGGTGATTTTGCTGGCGATTCCCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCgctg                                                                            <  1:168569/140‑1 (MQ=255)                                                tGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCgctgctgct                                                                       >  2:562052/1‑140 (MQ=255)                                                                     gTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGc                                                  >  2:552131/1‑140 (MQ=255)                                                                        tttttGCGGGGGATTCCCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGAc                                               <  2:578414/139‑1 (MQ=255)                                                                         ttttGCTGGCGATTCCCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGAcc                                              <  2:803250/140‑1 (MQ=255)                                                                                 gCGTTTCCCCCGCTGTGCCCCCGCGGGCTGACCATTTTCCCGAGGGGTGGTGATCCCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGAt                                      <  2:402253/140‑1 (MQ=255)                                                                                 gCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACcgc                                            <  2:108695/134‑1 (MQ=255)                                                                                 gCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACcgc                                            >  1:108695/1‑134 (MQ=255)                                                                                           cGCTGTGCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGa                            >  2:421076/1‑140 (MQ=255)                                                                                                tgCCGCCGCTGGCTGACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGACCGtt                       >  1:706170/1‑140 (MQ=255)                                                                                                        ctggctgACCATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGACCGTTGAGTTttg               >  2:599511/1‑140 (MQ=255)                                                                                                                 cATTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGACCGTTGAGTTTTGTTGGTTTaa      <  1:293864/140‑1 (MQ=255)                                                                                                                  aTTTTACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGACCGTTGAGTTTTGTTGGTTTAAt     >  1:190267/1‑140 (MQ=255)                                                                                                                     ttACCGATGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGACCGTTGAGTTTTGTTGGTTTAATGGc  <  2:278894/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                      TGACCTGGATTTCCGGTTCGACCTACAACGCGACCGATGCGCAGGTCTGGCGCACCGGAATTGTGATGGTGATTTTGCTGGCGATTACCCCGCTGTGCCGCCGCTGGCTGACCATTTTACCGCTGGGTGGTGATACCGCCCGAGCCGTAGGAATGGCGCTGACGCCGACGCGAATTGCGCTGCTGCTGTTAGCGGCTTGCCTGACGGCGACCGCGACGATGACTATTGGACCGTTGAGTTTTGTTGGTTTAATGGC  >  NC_000913/172987‑173242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑