Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,839,491	G→C	A9P (GCG→CCG)	ynjE →	molybdopterin synthase sulfurtransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,839,491	0	G	C	100.0%	134.2 / NA	36	A9P (GCG→CCG)	ynjE	molybdopterin synthase sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/17);  total (19/17)

GATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTATAACGGC  >  NC_000913/1839359‑1839625                                                                                                                                     |                                                                                                                                       gATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGc                                                                                                                                 >  1:455402/1‑140 (MQ=255)   tatGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAGTGCGTCAGTTCAGAATGGCGAACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCaa                                                                                                                               <  1:468653/140‑1 (MQ=255)   tatGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCaa                                                                                                                               <  1:455222/140‑1 (MQ=255)    atGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAAt                                                                                                                              <  2:529488/140‑1 (MQ=255)    atGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAAt                                                                                                                              <  1:228364/140‑1 (MQ=255)      ggCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATgg                                                                                                                            >  1:858508/1‑140 (MQ=255)      ggCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATgg                                                                                                                            >  2:778607/1‑140 (MQ=255)               gTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGggc                                                                                                                   >  1:202043/1‑140 (MQ=255)                  aGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGATTTAGggctgg                                                                                                                >  2:677780/1‑140 (MQ=255)                   gAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGggctggc                                                                                                               <  1:811883/140‑1 (MQ=255)                     aaaTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCtt                                                                                                             <  2:858508/140‑1 (MQ=255)                     aaaTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCtt                                                                                                             <  1:688316/140‑1 (MQ=255)                       aTTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTgc                                                                                                           >  2:282690/1‑140 (MQ=255)                                      gATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGcc                                                                                            >  2:627346/1‑140 (MQ=255)                                            gCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGaa                                                                                      <  2:274216/140‑1 (MQ=255)                                                          aaTGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCctct                                                                        <  1:282690/140‑1 (MQ=255)                                                                tCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACt                                                                  <  2:455402/140‑1 (MQ=255)                                                                   gTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGa                                                               <  1:530870/140‑1 (MQ=255)                                                                         aaTGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCt                                                         >  2:422440/1‑140 (MQ=255)                                                                             gcgcACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaa                                                     >  1:218190/1‑140 (MQ=255)                                                                              cgcACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaac                                                    >  1:499904/1‑140 (MQ=255)                                                                              cgcACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaac                                                    >  2:194182/1‑140 (MQ=255)                                                                                cACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTcaacaa                                                  >  1:21765/1‑140 (MQ=255)                                                                                       aaCTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATg                                           >  2:15174/1‑140 (MQ=255)                                                                                          tACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCa                                        <  2:202043/140‑1 (MQ=255)                                                                                          tACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCa                                        <  1:778607/140‑1 (MQ=255)                                                                                           aCAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCaa                                       >  2:156760/1‑140 (MQ=255)                                                                                               tGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCg                                   >  1:366848/1‑140 (MQ=255)                                                                                                 tCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgat                                 <  1:848749/140‑1 (MQ=255)                                                                                                   gggATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgatag                               <  2:499904/140‑1 (MQ=255)                                                                                                    ggATTTTCGATGAAACGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCgataga                              >  2:165897/1‑140 (MQ=255)                                                                                                                  aCGTGTTTCTCAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAgcg                <  2:127585/140‑1 (MQ=255)                                                                                                                          ctcAAATGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTtat        >  2:715092/1‑140 (MQ=255)                                                                                                                               aTGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTATAACgg   >  2:1632/1‑140 (MQ=255)                                                                                                                               aTGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTATAACgg   >  2:626770/1‑140 (MQ=255)                                                                                                                                tGACCCCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTATAACGGc  <  1:627346/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                       GATATGGCGCAGTGGTCAGAAAATTACAACAAACTGCGATAACGCAAAGTTTTTCTCAATGCGTCAGTTCAGAATGGCGCACTCAAAACTACAATGTCGGGATTTTCGATGAAACGTGTTTCTCAAATGACCGCGCTGGCAATGGCTTTAGGGCTGGCTTGCGCTTCTTCGTGGGCCGCTGAACTGGCGAAGCCTCTTACACTTGACCAGCTTCAACAACAAAATGGCAAAGCGATAGATACTCGCCCCAGCGCGTTTTATAACGGC  >  NC_000913/1839359‑1839625

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑