Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,967,672	C→A	G277V (GGG→GTG)	cheB ←	protein‑glutamate methylesterase/protein glutamine deamidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,967,672	0	C	A	100.0%	104.0 / NA	33	G277V (GGG→GTG)	cheB	protein‑glutamate methylesterase/protein glutamine deamidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/14);  total (19/14)

ATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTACGCGACAGCTCC  >  NC_000913/1967546‑1967800                                                                                                                                |                                                                                                                                 aTGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAGGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACg                                                                                                                     <  2:435860/140‑1 (MQ=255)   ggCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGcc                                                                                                                   >  2:611699/1‑140 (MQ=255)      cTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCg                                                                                                                >  1:837556/1‑140 (MQ=255)             ggCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGttt                                                                                                         >  1:655089/1‑140 (MQ=255)                    cgacccccacccacCTTGCTTCGTTTTGCGCAGGGGTCCAGGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACa                                                                                                  <  2:615/134‑1 (MQ=255)                       acacCCCGCAACTTGCTTCGTTTGGCGCAGGGGTCCATGCCCCC‑GCCGGACGCATCGCCAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGaa                                                                                               <  2:657329/140‑1 (MQ=255)                             cgaaccTTGCTTCGTTTGGCGCAGGGGCCCATGCCCCC‑GCCTGACGCATCGCCACCATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaac                                                                                         <  2:381780/135‑1 (MQ=255)                             cGCACCTTGCTTCGTTTGGCGCAGGGGCCCACGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaac                                                                                         <  2:570530/140‑1 (MQ=255)                             cGCAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaac                                                                                         >  1:762007/1‑140 (MQ=255)                             cGCAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGACGACAGAATGGaac                                                                                         >  1:777977/1‑140 (MQ=255)                               cAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGaacaa                                                                                       <  2:762007/140‑1 (MQ=255)                                 ccttccttcGTTTTGCGCAGGGGT‑CACGCCCCCGGCTTGACACACCCCAAACTTTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAacaaca                                                                                     <  2:655089/139‑1 (MQ=255)                                  cTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAacac                                                                                    >  1:674631/1‑140 (MQ=255)                                    tGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACAt                                                                                  <  1:711290/140‑1 (MQ=255)                                    gccTTTGTTTTGCGCAGGGTTCCTGCCCCCC‑CCCCGCCCCACCGCAAACATCCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACAt                                                                                  <  2:674631/138‑1 (MQ=255)                                     gCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATc                                                                                 >  1:696821/1‑140 (MQ=255)                                     gCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATc                                                                                 >  1:533213/1‑140 (MQ=255)                                              tGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAgg                                                                        <  1:328064/140‑1 (MQ=255)                                                               cccc‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCACATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAAccg                                                       >  1:629540/1‑140 (MQ=255)                                                                 cc‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAAccgcc                                                     >  2:232343/1‑140 (MQ=255)                                                                 cc‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAAccgcc                                                     >  2:408091/1‑140 (MQ=255)                                                                 cc‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAAccgcc                                                     >  1:261978/1‑140 (MQ=255)                                                                             aTCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTg                                          >  2:272463/1‑140 (MQ=255)                                                                                         cccGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTg                              >  2:284769/1‑140 (MQ=255)                                                                                           cgccgcgccgTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGt                            >  2:179668/1‑140 (MQ=255)                                                                                               gcgccgTCGTTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAAtt                        >  1:162629/1‑140 (MQ=255)                                                                                                    gtcgtTGCCCATACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTgcgc                   <  1:265063/140‑1 (MQ=255)                                                                                                             cATACCGGTCAGGATCACCACAACCACATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTAcgcg          <  2:629540/140‑1 (MQ=255)                                                                                                              aTACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTACGCGa         <  2:837556/140‑1 (MQ=255)                                                                                                              aTACCGGTCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTACGCGa         <  1:179668/140‑1 (MQ=255)                                                                                                                     tCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTACGCGACAGCTcc  >  2:648304/1‑140 (MQ=255)                                                                                                                     tCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTACGCGACAGCTcc  >  1:799154/1‑140 (MQ=255)                                                                                                                     tCAGGATCACCACAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTACGCGACAGCTcc  >  1:370044/1‑140 (MQ=255)                                                                                                                                |                                                                                                                                 ATGGCCTCGCGCGGCATGCCGAACACCACGCAACTTGCTTCGTTTTGCGCAAGGGTCCATGCCCCC‑GCCTGACGCATCGCCAACATTCCCGCCGCGCCGTCGTTGCCCATACCGGTCAGGATCACCCCAACCGCATTACGCCCCGCCTGTTTGGCGACAGAATGGAACAACACATCTACCGAAGGCCGATGACGGTTAACCGCCGGGCCATCGTGAATTTTGATTTGGTAATTTGCGCCACTACGCGACAGCTCC  >  NC_000913/1967546‑1967800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑