Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,312,221	G→T	P177T (CCA→ACA)	ompC ←	outer membrane porin C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,312,221	0	G	T	100.0%	196.7 / NA	56	P177T (CCA→ACA)	ompC	outer membrane porin C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/28);  total (28/28)

TTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCAC  >  NC_000913/2312084‑2312355                                                                                                                                          |                                                                                                                                       tttGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATgtgt                                                                                                                                      >  1:575310/1‑140 (MQ=255)   tGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTg                                                                                                                                    >  2:204842/1‑140 (MQ=255)    ggAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGc                                                                                                                                   >  2:822009/1‑140 (MQ=255)          ggAGCTCGCACCGCCGATACCGAACCCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGtttt                                                                                                                             <  1:257930/140‑1 (MQ=255)          ggAGATCGCCCCCCCGATACCGAACCCTTCGAAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGtttt                                                                                                                             <  2:716392/140‑1 (MQ=255)            agaTCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTa                                                                                                                           <  1:29170/140‑1 (MQ=255)               tCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGACACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTAccc                                                                                                                        <  1:287120/140‑1 (MQ=255)                  caccacCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTgg                                                                                                                     >  2:741761/1‑140 (MQ=255)                    ccaccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTa                                                                                                                   <  2:575310/140‑1 (MQ=255)                     caccGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTAc                                                                                                                  >  2:740345/1‑140 (MQ=255)                          aTACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAAc                                                                                                             >  1:479164/1‑140 (MQ=255)                          aTACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAAc                                                                                                             >  2:673785/1‑140 (MQ=255)                                     ttCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa                                                                                                  <  1:699150/140‑1 (MQ=255)                                     ttCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCa                                                                                                  <  1:439304/140‑1 (MQ=255)                                       cGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg                                                                                                >  2:139371/1‑140 (MQ=255)                                       cGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAgg                                                                                                >  1:702607/1‑140 (MQ=255)                                                 aGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                      <  1:822009/140‑1 (MQ=255)                                                 aGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCa                                                                                      >  2:437044/1‑140 (MQ=255)                                                     aTAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGCTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGAcc                                                                                  >  2:457835/1‑140 (MQ=255)                                                      tAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg                                                                                 >  1:415568/1‑140 (MQ=255)                                                        gAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaa                                                                               >  1:530215/1‑140 (MQ=255)                                                          aCCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCTCCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCgaaga                                                                             >  1:284526/1‑140 (MQ=255)                                                             gccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                                          <  1:533883/140‑1 (MQ=255)                                                             gccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                                          <  1:673785/140‑1 (MQ=255)                                                             gccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                                          <  1:740345/140‑1 (MQ=255)                                                             gccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                                          <  1:741761/140‑1 (MQ=255)                                                             gccgACGCCGTCGCCGTTTTGACGCAGAGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGt                                                                          >  2:404653/1‑140 (MQ=255)                                                              ccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTTCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                                                         <  1:404653/140‑1 (MQ=255)                                                              ccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                                                         <  2:479164/140‑1 (MQ=255)                                                              ccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                                                         <  2:530215/140‑1 (MQ=255)                                                              ccgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTc                                                                         >  2:837355/1‑140 (MQ=255)                                                               cgACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCa                                                                        >  2:758078/1‑140 (MQ=255)                                                                  cgccgtcgccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtg                                                                     <  2:415568/140‑1 (MQ=255)                                                                   gccgtcgccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAgtgt                                                                    <  1:139371/140‑1 (MQ=255)                                                                        cgccgtTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACgg                                                               >  1:624135/1‑140 (MQ=255)                                                                                   cGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGaa                                                    <  1:204842/140‑1 (MQ=255)                                                                                        tGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                               >  2:138970/1‑140 (MQ=255)                                                                                        tGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGt                                               >  2:381546/1‑140 (MQ=255)                                                                                             cACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa                                          <  1:437044/140‑1 (MQ=255)                                                                                             cACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCa                                          >  2:563654/1‑140 (MQ=255)                                                                                                  ccGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACgctg                                     >  1:503354/1‑140 (MQ=255)                                                                                                         tagttaCGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATg                              <  1:138970/140‑1 (MQ=255)                                                                                                           gttaCGCCACTAGTAAAGCCTTCACCAGATGTGCTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGaa                            <  1:457835/140‑1 (MQ=255)                                                                                                            ttaCGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAg                           >  1:697000/1‑140 (MQ=255)                                                                                                              aCGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGtt                         >  2:538860/1‑140 (MQ=255)                                                                                                               cGCCACTAGTAAAGCCTTCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTg                        >  2:544711/1‑140 (MQ=255)                                                                                                                     tAGTAAAGCCTTCACCAGATGTATTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGaa                  >  2:328577/1‑140 (MQ=255)                                                                                                                               ttCACCGGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACAAGACCGGAGAAGGCAGTGTTACGGTAGGTCGAGAAGCCGTTACAACGCGGCTGCATGAAGTAGTCAGCACCGTAggcga        >  2:801191/1‑137 (MQ=255)                                                                                                                               ttCACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGgtgt        >  1:488012/1‑140 (MQ=255)                                                                                                                                 cACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTc                      <  2:289580/124‑1 (MQ=255)                                                                                                                                 cACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTc                      >  1:289580/1‑124 (MQ=255)                                                                                                                                 cACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTca      >  1:600685/1‑140 (MQ=255)                                                                                                                                 cACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTca      <  1:758078/140‑1 (MQ=255)                                                                                                                                 cACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTca      <  1:381546/140‑1 (MQ=255)                                                                                                                                 cACCAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTca      >  2:500858/1‑140 (MQ=255)                                                                                                                                   ccAGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcacc    <  2:600685/140‑1 (MQ=255)                                                                                                                                     aGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac  <  1:500858/140‑1 (MQ=255)                                                                                                                                     aGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac  <  2:81762/140‑1 (MQ=255)                                                                                                                                     aGATGTGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTcaccac  <  1:544711/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       TTTGGAGCTGGAGATCGCACCACCGATACCGAAACCTTCGTAATCATAAGTGATAGAACCGCCGACGCCGTCGCCGTTTTGACGCAGTGCGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCGAAGAAGTCAGTGTTACGGTAGGTCGCGAAGCCGTTACCACGCTGCTGCATGAAGTTGTCAGAACCGTAGGTGTCACCAC  >  NC_000913/2312084‑2312355

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑