Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,363,183	G→T	P151T (CCC→ACC)	yfaY ←	IPR008135 CinA family protein YfaY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,363,183	0	G	T	100.0%	83.6 / NA	25	P151T (CCC→ACC)	yfaY	IPR008135 CinA family protein YfaY
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (10/15);  total (10/15)

GTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACG  >  NC_000913/2363048‑2363315                                                                                                                                        |                                                                                                                                     gtgCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGgtagta                                                                                                                                  >  2:733586/1‑140 (MQ=255) gtgCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGgtagta                                                                                                                                  >  2:723736/1‑140 (MQ=255)        aTCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACa                                                                                                                           >  2:96626/1‑140 (MQ=255)              ttCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGc                                                                                                                     <  2:388172/140‑1 (MQ=255)                                  tCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCa                                                                                                 <  1:300009/140‑1 (MQ=255)                                            aCAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAgg                                                                                       <  1:723736/140‑1 (MQ=255)                                                     cggcTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTa                                                                              <  1:373880/140‑1 (MQ=255)                                                      ggcTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACAGTACGCCGGTAGTAAAGAACATCAGGCAGCGATTAAGCTGCTCGTCAAATCCCCAGTAGGTGCATat                                                                             <  2:590413/140‑2 (MQ=255)                                                           cGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGt                                                                        >  1:109356/1‑140 (MQ=255)                                                           cGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGt                                                                        <  1:230084/140‑1 (MQ=255)                                                                          cTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAg                                                         >  1:569300/1‑140 (MQ=255)                                                                                cAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTgg                                                   <  1:604657/140‑1 (MQ=255)                                                                                         cAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCt                                          >  1:776238/1‑140 (MQ=255)                                                                                         cAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCt                                          >  1:367420/1‑140 (MQ=255)                                                                                             aTTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGc                                      <  1:286663/140‑1 (MQ=255)                                                                                                  gTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGtt                                 <  2:776238/140‑1 (MQ=255)                                                                                                  gTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGtt                                 <  2:367420/140‑1 (MQ=255)                                                                                                    gCTCGACCATCACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTa                               <  2:569300/140‑1 (MQ=255)                                                                                                              cACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAATCCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCg                     >  1:782281/1‑140 (MQ=255)                                                                                                              cACCTTAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCg                     <  1:96626/140‑1 (MQ=255)                                                                                                                   tAAATTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGcc                <  1:733586/140‑1 (MQ=255)                                                                                                                    aaaTTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTc                      >  2:421365/1‑133 (MQ=255)                                                                                                                    aaaTTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTc                      <  1:421365/133‑1 (MQ=255)                                                                                                                    aaaTTCTGACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCa               >  1:792264/1‑140 (MQ=255)                                                                                                                           gACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCg        >  1:143328/1‑140 (MQ=255)                                                                                                                           gACGGTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCg        >  1:737493/1‑140 (MQ=255)                                                                                                                               gTACGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCa    <  2:96899/140‑1 (MQ=255)                                                                                                                                 aCGCCGGTAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACg  <  2:109356/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GTGCCAGATCGCTTTCCGAACGACCAAAAGTAGTCAAACGCAGACAAACCGGCGGCTGCGGTAAAGAAAAACGCTCGCGCAGGCGCGGCAGGATTTCGTGCTCGACCATCACCTTAAATTCTGACGGTACGCCGGGAGTAAAGAACATCAGGCAACGATTAAGCTGCACGGCAAAACCACAGGCGGTGCCTACCGGGTTATTGATAAATTCAGCACTGGCAGGCAGCTCCGCTTGTTTACGGTTGCTCGGTGCCATTACTCGTCCACG  >  NC_000913/2363048‑2363315

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑