Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,898,944	G→T	R159R (CGA→AGA)	ygcU ←	putative FAD‑containing dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,898,944	0	G	T	100.0%	224.6 / NA	63	R159R (CGA→AGA)	ygcU	putative FAD‑containing dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/35);  total (28/35)

TGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCA  >  NC_000913/2898808‑2899080                                                                                                                                         |                                                                                                                                         tgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGt                                                                                                                                       >  2:579033/1‑140 (MQ=255) tgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGt                                                                                                                                       <  1:579033/140‑1 (MQ=255) tgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGt                                                                                                                                       <  1:569913/140‑1 (MQ=255) tgACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGt                                                                                                                                       <  2:79137/140‑1 (MQ=255)       aTGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTAc                                                                                                                                 >  2:285781/1‑140 (MQ=255)           ccGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGTCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAgg                                                                                                                             >  2:775633/1‑140 (MQ=255)           ccGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGTTACCAga                                                                                                                             <  2:771894/140‑2 (MQ=255)                    ccGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATc                                                                                                                    >  1:105843/1‑140 (MQ=255)                      gcgcgGCGTGGCACGTTTTTAATGCGTGTGACGGTGTCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTg                                                                                                                  <  1:775633/140‑1 (MQ=255)                                         tAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGc                                                                                               <  1:145119/140‑1 (MQ=255)                                          aaTGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCg                                                                                              <  1:739816/140‑1 (MQ=255)                                            tGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGga                                                                                            <  1:285781/140‑1 (MQ=255)                                                tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGa                                                                                                   <  2:713196/129‑1 (MQ=255)                                                tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGa                                                                                                   >  1:713196/1‑129 (MQ=255)                                                tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAAt                                                                                        >  2:328226/1‑140 (MQ=255)                                                tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAAt                                                                                        >  2:209469/1‑140 (MQ=255)                                                tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAAt                                                                                        >  2:379611/1‑140 (MQ=255)                                                tgtgACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAAt                                                                                        <  2:441121/140‑1 (MQ=255)                                                     cGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcccc                                                                                   <  2:469423/140‑1 (MQ=255)                                                     cGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGcccc                                                                                   <  1:727273/140‑1 (MQ=255)                                                      ggTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCg                                                                                  <  2:237065/140‑1 (MQ=255)                                                      ggTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCg                                                                                  <  1:408476/140‑1 (MQ=255)                                                          ccATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCgtggt                                                                              <  2:604455/140‑1 (MQ=255)                                                                    aTACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCtttt                                                                    >  1:796333/1‑140 (MQ=255)                                                                         gCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACg                                                               >  1:172233/1‑140 (MQ=255)                                                                          cTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGc                                                              >  1:456001/1‑140 (MQ=255)                                                                           ttCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCa                                                             <  2:654204/140‑1 (MQ=255)                                                                             ccAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAAc                                                           <  1:209469/140‑1 (MQ=255)                                                                               aGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAAcgc                                                         >  2:841776/1‑140 (MQ=255)                                                                                 aCCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGt                                                       >  2:579783/1‑140 (MQ=255)                                                                                    aaCGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGtttt                                                    >  2:518668/1‑140 (MQ=255)                                                                                      cGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTcc                                                  <  1:328226/140‑1 (MQ=255)                                                                                           atatCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCAc                                             >  2:650296/1‑140 (MQ=255)                                                                                           atatCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCAc                                             >  1:462379/1‑140 (MQ=255)                                                                                              tCTTCGATTGCGCGGTAGAGTGTGGAGAACTGCCCGATCCTTCTGGTTGCTCCCAGGCCGCCCATCTGCCCCAGCGGCTTTGCTTGCGGAGAATGCCCCGGGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTc                                          <  2:313719/140‑1 (MQ=255)                                                                                              tCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTc                                          <  1:317414/140‑1 (MQ=255)                                                                                                 tCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAg                                       <  1:18603/140‑1 (MQ=255)                                                                                                   gATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCg                                     <  1:711505/140‑1 (MQ=255)                                                                                                   gATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCg                                     >  2:711505/1‑140 (MQ=255)                                                                                                   gATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCg                                     <  1:359277/140‑1 (MQ=255)                                                                                                   gATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCg                                     <  2:775640/140‑1 (MQ=255)                                                                                                        cgcCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCTGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca                                >  1:735420/1‑140 (MQ=255)                                                                                                        cgcCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca                                >  1:826564/1‑140 (MQ=255)                                                                                                        cgcCGTAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAaca                                >  1:313719/1‑140 (MQ=255)                                                                                                             tAGAGTGTGGAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACcaca                           >  2:45355/1‑140 (MQ=255)                                                                                                                     ggAGAACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCg                   <  2:735420/140‑1 (MQ=255)                                                                                                                      gagaACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGt                  <  1:518668/140‑1 (MQ=255)                                                                                                                       agaACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTc                 >  1:377755/1‑140 (MQ=255)                                                                                                                       agaACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGAGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTc                 >  1:205919/1‑140 (MQ=255)                                                                                                                        gaACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCg                <  2:377755/140‑1 (MQ=255)                                                                                                                        gaACTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCg                <  1:385247/140‑1 (MQ=255)                                                                                                                          aCTGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGcc              >  2:542031/1‑140 (MQ=255)                                                                                                                            tGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg            >  2:89891/1‑140 (MQ=255)                                                                                                                            tGCCCGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTg            >  1:83476/1‑140 (MQ=255)                                                                                                                              cccGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCa          >  1:283279/1‑140 (MQ=255)                                                                                                                                cGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCata        <  2:593341/140‑1 (MQ=255)                                                                                                                                cGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCata        >  1:9931/1‑140 (MQ=255)                                                                                                                                cGATACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCata        <  2:826564/140‑1 (MQ=255)                                                                                                                                  aTACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATAtt      <  2:456001/140‑1 (MQ=255)                                                                                                                                  aTACTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATAtt      <  1:841776/140‑1 (MQ=255)                                                                                                                                    aCTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtct    <  1:89891/140‑1 (MQ=255)                                                                                                                                    aCTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtct    <  2:796333/140‑1 (MQ=255)                                                                                                                                     cTTCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATtctc   <  2:105843/140‑1 (MQ=255)                                                                                                                                      ttCTGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCtca  <  2:283279/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TGACGAATGTCCGGGCCAGCCGCGCGGCGTGGCACGTTTTTAATGCGTGTGACGGTGCCATCAGCCAATACTGCTTCCAGACCAACGACCATATCTTCGATTGCGCCGTAGAGTGTGGAGAACTGCCCGATACTTCGGGTTGCTACCAGGCCGCCCATCTGCGCCAGCGGCTTTGACTGCGGAGAATGCCCCGTGGTGTAACCTTTTTCACGCAACGCGTTTTCCAGCACTTCCAGCGGAACACCACATTGCGCCGTCGCCTGCATATTCTCA  >  NC_000913/2898808‑2899080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑