Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,135,446	G→T	A309E (GCA→GAA)	pitB ←	metal phosphate:H(+) symporter PitB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,135,446	0	G	T	100.0%	124.6 / NA	36	A309E (GCA→GAA)	pitB	metal phosphate:H(+) symporter PitB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (13/23);  total (13/23)

CTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCG  >  NC_000913/3135313‑3135580                                                                                                                                       |                                                                                                                                       cTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGAt                                                                                                                                  <  1:140793/140‑1 (MQ=255)         aGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGa                                                                                                                          <  1:855586/140‑1 (MQ=255)                   gaTGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTa                                                                                                                >  1:53207/1‑140 (MQ=255)                    aTGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTAc                                                                                                               <  1:45005/140‑1 (MQ=255)                     tGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACt                                                                                                              >  1:304272/1‑140 (MQ=255)                     tGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACt                                                                                                              <  2:304272/140‑1 (MQ=255)                          aGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGt                                                                                                         <  1:684096/140‑1 (MQ=255)                              tAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCa                                                                                                     >  2:798695/1‑140 (MQ=255)                              tAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCa                                                                                                     <  1:798695/140‑1 (MQ=255)                                           ctggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑AGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAtgctg                                                                                        >  2:301902/1‑140 (MQ=255)                                            tggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAtgctgc                                                                                       <  2:548701/140‑1 (MQ=255)                                            tggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGAtgctgc                                                                                       <  1:504878/140‑1 (MQ=255)                                             ggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCa                                                                                      <  2:123504/140‑1 (MQ=255)                                             ggctgCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCa                                                                                      <  1:82462/140‑1 (MQ=255)                                                      gACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGa                                                                             <  2:53207/140‑1 (MQ=255)                                                            cGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGGATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTcc                                                                       >  1:556027/1‑140 (MQ=255)                                                              cTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCAt                                                                     <  2:815799/140‑1 (MQ=255)                                                                 aaCGGCTCGTAACTTTTCATATTTCCTGGCAGGAACGTTTTAACCCGCGCAATAGGATCAAAGGGGATTTTCC‑GATGACAGCGGAAACCTGTTAATTGCCTGCCATCAGTCGATGCTGCAAGGAATGGGGGGTCCCTCGc                                                                  >  2:578519/1‑140 (MQ=255)                                                                       tCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCaa                                                            >  1:310605/1‑140 (MQ=255)                                                                                tCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCgg                                                   <  2:265640/140‑1 (MQ=255)                                                                                tCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCgg                                                   >  1:852327/1‑140 (MQ=255)                                                                                tCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCgg                                                   <  1:480152/140‑1 (MQ=255)                                                                                  cATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCa                                                 <  1:745088/140‑1 (MQ=255)                                                                                     atTGCCTGGTAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGtt                                              <  2:402512/140‑1 (MQ=255)                                                                                         ccTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAgg                                          >  2:183195/1‑140 (MQ=255)                                                                                          cTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGa                                         <  1:578519/140‑1 (MQ=255)                                                                                           tGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGAt                                        >  1:725524/1‑140 (MQ=255)                                                                                           tGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGAt                                        >  2:312836/1‑140 (MQ=255)                                                                                            ggCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGa                                         >  1:616758/1‑138 (MQ=255)                                                                                            ggCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGa                                         <  2:616758/138‑1 (MQ=255)                                                                                              cagcaTCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCt                                     <  2:310605/140‑1 (MQ=255)                                                                                              cagcaTCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCt                                     >  2:319915/1‑140 (MQ=255)                                                                                                               gcgcAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCg                    <  2:725524/140‑1 (MQ=255)                                                                                                                cgcAATAGCATCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGa                   >  2:156122/1‑140 (MQ=255)                                                                                                                         aTCAAA‑GGTATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTaa          <  1:312836/140‑1 (MQ=255)                                                                                                                                  tATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATcg  <  1:452765/140‑1 (MQ=255)                                                                                                                                  tATTTTCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATcg  <  1:236439/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       CTTCGCGGAGGTATCAGAGATGCACAGCATAATGCGGCGCAGCTGGCTGCGCTGACTCACGCTTAACGGCTCGTAACTTTCCATATTGCCTGGCAGCATCGTTTTAACGCGCGCAATAGCATCAAA‑GGTATTTGCCGGATGACAGTGAAACTCTGTTACTTGCGTGCCATCAGTCGATGCTGCAGGCAATGGAGGTTCCATCGCAATCAACTTCTGCGGCAGTTCAGGATGCTGTTGCAGGTAGTGTTCGAAGTTGGTAACGGCATCG  >  NC_000913/3135313‑3135580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑