Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,204,883	C→A	L64M (CTG→ATG)	plsY →	putative glycerol‑3‑phosphate acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,204,883	0	C	A	100.0%	116.7 / NA	34	L64M (CTG→ATG)	plsY	putative glycerol‑3‑phosphate acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/22);  total (12/22)

TTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCG  >  NC_000913/3204747‑3205015                                                                                                                                         |                                                                                                                                     ttCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGa                                                                                                                                   <  1:616935/140‑1 (MQ=255)            ttCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTg                                                                                                                        <  2:46057/140‑1 (MQ=255)            ttCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTg                                                                                                                        <  1:331396/140‑1 (MQ=255)            ttCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTg                                                                                                                        <  2:498055/140‑1 (MQ=255)            ttCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTg                                                                                                                        >  1:730727/1‑140 (MQ=255)              cTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGcc                                                                                                                      <  2:730727/140‑1 (MQ=255)              cTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGcc                                                                                                                      <  1:57348/140‑1 (MQ=255)               tGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGccc                                                                                                                     <  1:743856/140‑1 (MQ=255)                ggTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCg                                                                                                                    <  1:859944/140‑1 (MQ=255)                  tttGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCCGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTc                                                                                                                  <  1:642082/140‑1 (MQ=255)                      ccGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTggg                                                                                                              >  1:599061/1‑140 (MQ=255)                           tgtgtgGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGcgcg                                                                                                         <  2:529605/140‑1 (MQ=255)                           tgtgtgGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGcgcg                                                                                                         <  2:593375/140‑1 (MQ=255)                           tgtgtgGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGcgcg                                                                                                         >  1:789721/1‑140 (MQ=255)                                     gCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAg                                                                                               <  2:789721/140‑1 (MQ=255)                                      cccGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAgg                                                                                              >  1:634437/1‑140 (MQ=255)                                             cgcgAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGc                                                                                       <  1:71999/140‑1 (MQ=255)                                             cgcgAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGc                                                                                       <  2:492780/140‑1 (MQ=255)                                                gAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGc                                                                                       <  1:473488/137‑1 (MQ=255)                                                gAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGc                                                                                       >  2:473488/1‑137 (MQ=255)                                                 aaCCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCt                                                                                   <  2:70630/140‑1 (MQ=255)                                                          cTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGTAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAg                                                                          <  2:790472/140‑1 (MQ=255)                                                                             aCCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATcgccgc                                                       >  2:548240/1‑140 (MQ=255)                                                                             aCCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATcgccgc                                                       <  1:548240/140‑1 (MQ=255)                                                                             aCCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATcgccgc                                                       <  1:465148/140‑1 (MQ=255)                                                                             aCCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATcgccgc                                                       <  1:105281/140‑1 (MQ=255)                                                                             aCCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATcgacgc                                                       <  2:634437/140‑1 (MQ=255)                                                                                       tACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGa                                             <  2:373423/140‑1 (MQ=255)                                                                                              cGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCt                                      >  1:661411/1‑140 (MQ=255)                                                                                              cGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCt                                      >  2:720878/1‑140 (MQ=255)                                                                                                gTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTgg                                    >  1:626823/1‑140 (MQ=255)                                                                                                           gcagcCGTAGCAGTACTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTttctt                         >  1:133432/1‑140 (MQ=255)                                                                                                                         aCTGATTTTCGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAaggag           >  2:136315/1‑140 (MQ=255)                                                                                                                                  cGACGTTATGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTAAAGGGAGGAAAAGGCg  >  2:238322/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     TTCCAGTGCCATTCTGGTTTGCCGCTTGTGTGGGCTGCCCGATCCGCGAACCAGCGGCTCCGGCAATCCAGGCGCAACCAATGTGTTACGTATCGGTGGCAAGGGAGCAGCCGTAGCAGTACTGATTTTCGACGTTCTGAAAGGAATGTTGCCCGTCTGGGGCGCGTATGAATTAGGTGTCAGCCCCTTCTGGCTAGGCTTAATTGCCATCGCCGCCTGTCTTGGACACATCTGGCCCGTTTTCTTCGGATTTAAAGGAGGAAAAGGCG  >  NC_000913/3204747‑3205015

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑