Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,244,141	C→A	R312L (CGC→CTC)	malF ←	maltose ABC transporter membrane subunit MalF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,244,141	0	C	A	100.0%	250.5 / NA	71	R312L (CGC→CTC)	malF	maltose ABC transporter membrane subunit MalF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (29/42);  total (29/42)

CACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAG  >  NC_000913/4244005‑4244275                                                                                                                                         |                                                                                                                                       cACGCCAAACAGCGCGCTCGACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCa                                                                                                                                     <  1:859534/140‑1 (MQ=255)    gCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACg                                                                                                                                  <  1:614425/140‑1 (MQ=255)       aaaCAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCtt                                                                                                                               >  1:470049/1‑140 (MQ=255)           aGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCa                                                                                                                           <  1:750877/140‑1 (MQ=255)                gcTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCa                                                                                                                      <  1:760007/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCATAATTTAATTGAGCGATAGCGCCACGGTGGGCAAAATTTACAGGACGCGATAGCCCGCTTTTCCGATCAACTCTTCCCACTGCACCAGa                                                                                                                 <  2:620386/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  1:115737/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  1:763099/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  1:42615/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  2:362467/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  2:521243/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  2:624710/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  2:626024/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  2:708923/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  2:809520/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGACGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  2:742839/140‑1 (MQ=255)                     aCATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGAGTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  1:81974/140‑1 (MQ=255)                              tGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGa                                                                                                        >  2:642874/1‑140 (MQ=255)                                aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAAc                                                                                                      <  2:470049/140‑1 (MQ=255)                                aTTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAAc                                                                                                      >  2:141934/1‑140 (MQ=255)                                   tCACCGAAGCTCTGGTTAAAGAAGCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACAAGACACGCCAGAACCAt                                                                                                   >  2:647013/1‑140 (MQ=255)                                     aCCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 >  2:689174/1‑124 (MQ=255)                                     aCCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGa                                                                                                                 <  1:689174/124‑1 (MQ=255)                                     aCCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGc                                                                                                 >  2:228485/1‑140 (MQ=255)                                            ctctGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGc                                                                                          <  1:97917/140‑1 (MQ=255)                                            ctctGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGc                                                                                          <  1:135556/140‑1 (MQ=255)                                            ctctGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGc                                                                                          <  1:702291/140‑1 (MQ=255)                                                     aacaacCCTTTGAAAATCAAGATTGAAATGAACGAGGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTaa                                                                                 <  1:167584/140‑1 (MQ=255)                                                      acaacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTaaa                                                                                >  1:740481/1‑140 (MQ=255)                                                      acaacCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTaaa                                                                                >  1:313041/1‑140 (MQ=255)                                                         acCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAg                                                                             >  1:262430/1‑140 (MQ=255)                                                          cccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGa                                                                            <  1:150753/140‑1 (MQ=255)                                                          cccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGa                                                                            <  1:316965/140‑1 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCAcc                                                                                      <  2:407903/129‑1 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCAcc                                                                                      >  1:407903/1‑129 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                           >  2:761896/1‑140 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                           >  1:345813/1‑140 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                           >  1:369550/1‑140 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                           <  1:562129/140‑1 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGAc                                                                           >  2:562129/1‑140 (MQ=255)                                                           ccTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGAC‑CGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACa                                                                          >  2:602986/1‑140 (MQ=255)                                                            cTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCGGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACa                                                                          >  1:846560/1‑140 (MQ=255)                                                               tGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTg                                                                       >  1:646382/1‑140 (MQ=255)                                                                gAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGa                                                                      <  2:646382/140‑1 (MQ=255)                                                                gAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGa                                                                      <  2:224788/140‑1 (MQ=255)                                                                gAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGa                                                                      >  1:719381/1‑140 (MQ=255)                                                                     tCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGc                                                                 <  1:642874/140‑1 (MQ=255)                                                                       aaGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCGGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATTAGCga                                                               <  2:846560/140‑1 (MQ=255)                                                                         gATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCgaga                                                             >  1:808229/1‑140 (MQ=255)                                                                          aTTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGaa                                                            <  1:141934/140‑1 (MQ=255)                                                                          aTTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACAAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGaa                                                            <  1:647013/140‑1 (MQ=255)                                                                               aaTGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAAcacca                                                       <  2:262430/140‑1 (MQ=255)                                                                               aaTGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAAcacca                                                       <  1:761896/140‑1 (MQ=255)                                                                                            aCCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGAC‑CGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGaaa                                         <  1:602986/140‑1 (MQ=255)                                                                                              cgcgTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGaaaa                                        >  1:800428/1‑140 (MQ=255)                                                                                                    gggCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAA‑CATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAg                                 <  1:276017/140‑1 (MQ=255)                                                                                                       cAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGa                               >  1:482946/1‑140 (MQ=255)                                                                                                       cAGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGa                               <  1:163850/140‑1 (MQ=255)                                                                                                        aGAATCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGaa                              <  1:714388/140‑1 (MQ=255)                                                                                                          aaTCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACg                            <  2:381235/140‑1 (MQ=255)                                                                                                          aaTCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACg                            >  2:482208/1‑140 (MQ=255)                                                                                                          aaTCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACg                            >  2:722908/1‑140 (MQ=255)                                                                                                           aTCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACgg                           <  2:719381/140‑1 (MQ=255)                                                                                                            tCAGCAGGACGCGATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGt                          <  2:808229/140‑1 (MQ=255)                                                                                                                     cgcgATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAATAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATg                 >  2:523609/1‑140 (MQ=255)                                                                                                                     cgcgATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGaa                              >  2:722492/1‑127 (MQ=255)                                                                                                                     cgcgATAGACCGCTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGaa                              <  1:722492/127‑1 (MQ=255)                                                                                                                                 cTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTa     >  1:143237/1‑140 (MQ=255)                                                                                                                                 cTTTGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTa     >  1:5962/1‑140 (MQ=255)                                                                                                                                  tttGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTaa    >  1:258756/1‑140 (MQ=255)                                                                                                                                    tGCCGAGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAg  >  1:221410/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CACGCCAAACAGCGCGCTCAACATCATGTTGATTTCACCGAAGCTCTGGTTAAACAACCCTTTGAAAATCAAGATTGAAATGAACGATGGCACCGCGTAGGGCAGAATCAGCAGGACGCGATAGACCGCTTTGCCGCGCAACGCTTCCCACTGCACCAGACACGCCAGAACCATGCCGACCGCCACCGTTAAAAAGACAGTGATCAGCGAGAACACCACGGTCCAGACGAAAATGGCGAGGAACGGTTTCTGAATGCCTTCGTCGGTAAAG  >  NC_000913/4244005‑4244275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑