Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,382,280	G→T	A13A (GCC→GCA)	frdA ←	fumarate reductase flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,382,280	0	G	T	100.0%	213.7 / NA	60	A13A (GCC→GCA)	frdA	fumarate reductase flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/31);  total (29/31)

TGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATA  >  NC_000913/4382146‑4382415                                                                                                                                       |                                                                                                                                        tGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGcc                                                                                                                                    <  1:358756/140‑1 (MQ=255)  gcgcGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCt                                                                                                                                   >  1:12148/1‑140 (MQ=255)  gcgcGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCt                                                                                                                                   >  2:557966/1‑140 (MQ=255)    gcgACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTAc                                                                                                                                 >  2:817467/1‑140 (MQ=255)        cAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATg                                                                                                                             >  1:77950/1‑140 (MQ=255)        cAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATg                                                                                                                             >  1:373034/1‑140 (MQ=255)        cAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATg                                                                                                                             >  2:70001/1‑140 (MQ=255)             gcggAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAg                                                                                                                        >  1:430467/1‑140 (MQ=255)                gAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATc                                                                                                                     <  2:175827/140‑1 (MQ=255)                  gCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCgg                                                                                                                   >  1:485957/1‑140 (MQ=255)                        ttCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGaa                                                                                                             <  1:606665/140‑1 (MQ=255)                            gcagcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGt                                                                                                         <  2:294487/140‑1 (MQ=255)                             cagcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATc                                                                                                                     <  2:513089/127‑1 (MQ=255)                             cagcaACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATc                                                                                                                     >  1:513089/1‑127 (MQ=255)                                  aCGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCAc                                                                                                   >  2:830154/1‑140 (MQ=255)                                    ggTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGa                                                                                                 <  2:12148/140‑1 (MQ=255)                                           cTACGCATC‑GGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATtcctc                                                                                         >  2:133462/1‑140 (MQ=255)                                              cGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCa                                                                                       >  2:248900/1‑140 (MQ=255)                                               gCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAg                                                                                      >  2:77818/1‑140 (MQ=255)                                                cATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGa                                                                                     <  1:606367/140‑1 (MQ=255)                                                cATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGa                                                                                     <  1:830154/140‑1 (MQ=255)                                                cATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGa                                                                                     <  2:110146/140‑1 (MQ=255)                                                 aTCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGAt                                                                                    <  2:394704/140‑1 (MQ=255)                                                              ttGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCca                                                                       >  1:651410/1‑140 (MQ=255)                                                                gagaTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCcaca                                                                     >  2:348747/1‑140 (MQ=255)                                                                gagaTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACCACATTCCTCCAGATTGTTTTTATCCcaca                                                                     >  2:58294/1‑140 (MQ=255)                                                                   aTTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGcc                                                                  >  2:365004/1‑140 (MQ=255)                                                                     tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCAc                                                                <  1:557966/140‑1 (MQ=255)                                                                     tAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCAc                                                                <  2:688454/140‑1 (MQ=255)                                                                        tGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTa                                                             >  2:198321/1‑140 (MQ=255)                                                                            aTTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTc                                                         <  2:624432/140‑1 (MQ=255)                                                                             tttttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCa                                                        >  2:448111/1‑140 (MQ=255)                                                                                ttGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAggg                                                     >  1:257029/1‑140 (MQ=255)                                                                                 tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACg                                                               >  1:570573/1‑129 (MQ=255)                                                                                 tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACg                                                               <  2:570573/129‑1 (MQ=255)                                                                                 tGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGt                                                    <  2:454773/140‑1 (MQ=255)                                                                                   cATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTaa                                                  <  2:485957/140‑1 (MQ=255)                                                                                    aTTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAg                                                 <  1:817467/140‑1 (MQ=255)                                                                                             tGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCCCAGCCACGTACTTCAGGGGAAGTACCTGaaa                                        >  2:410438/1‑140 (MQ=255)                                                                                                cTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGtt                                     <  2:430467/140‑1 (MQ=255)                                                                                                 tGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTa                                    <  1:627463/140‑1 (MQ=255)                                                                                                    gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACgg                                 >  1:39841/1‑140 (MQ=255)                                                                                                    gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACgg                                 <  1:77818/140‑1 (MQ=255)                                                                                                    gcgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACgg                                 >  1:167965/1‑140 (MQ=255)                                                                                                     cgGCAGCAATTGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGt                                <  1:70001/140‑1 (MQ=255)                                                                                                              ttGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACg                       >  1:301439/1‑140 (MQ=255)                                                                                                               tGCAGCACGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGc                      <  1:198321/140‑1 (MQ=255)                                                                                                                  agcaCGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTAt                   <  2:39841/140‑1 (MQ=255)                                                                                                                    caCGTAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTc                 >  1:820658/1‑140 (MQ=255)                                                                                                                        tAATCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACt             <  2:301439/140‑1 (MQ=255)                                                                                                                         aaTCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTg            <  2:651410/140‑1 (MQ=255)                                                                                                                          aTCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGc           <  1:133462/140‑1 (MQ=255)                                                                                                                           tCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCTGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCt          >  1:344711/1‑140 (MQ=255)                                                                                                                           tCCCGCGCCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCt          <  2:820658/140‑1 (MQ=255)                                                                                                                                cgcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTc         <  2:191230/136‑1 (MQ=255)                                                                                                                                cgcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTc         >  1:191230/1‑136 (MQ=255)                                                                                                                                 gcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTta    <  1:24950/140‑1 (MQ=255)                                                                                                                                 gcCACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGCTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTta    >  2:99502/1‑140 (MQ=255)                                                                                                                                  ccACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTtat   <  1:248900/140‑1 (MQ=255)                                                                                                                                   cACCTGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTtata  <  1:348747/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        TGCGCGACAGCGGCGGAGCCCCCTTCTGCAGCAACGGTATGGCTACGCATCGGGTATACTTTTGAGATTAGTGCGATTTTTGCATTCGGATTTGCCTGCGCGGCAGCAATTGCAGCACGTAATCCCGCGCCACCGGCGCCTACAATGGCAAGATCGGCTTGAAAGGTTTGCACGACATTCCTCCAGATTGTTTTTATCCCACAGCCACGTACTTCAGGGTAAGTACCTGAAAGTTACGGTCTGCGAACGCTATTCCACTGCTCCTTTATA  >  NC_000913/4382146‑4382415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑